A5027543861- 3D Printing in Biomedical Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neural Engineering
- Barrier Structure and Function Studies
- Anesthesia and Neurotoxicity Research
- Pluripotent Stem Cells Research
- Drug Transport and Resistance Mechanisms
- Neuropeptides and Animal Physiology
- Neurogenesis and neuroplasticity mechanisms
- Diet and metabolism studies
- Advanced Scientific Research Methods
- Receptor Mechanisms and Signaling
- Innovative Microfluidic and Catalytic Techniques Innovation
- Regional resilience and development
- Hydrogen embrittlement and corrosion behaviors in metals
- Criminal Law and Policy
- Caveolin-1 and cellular processes
- Engineering Technology and Methodologies
- Neurological Disorders and Treatments
- Bone Tissue Engineering Materials
- Food Industry and Aquatic Biology
- German Security and Defense Policies
- Fatigue and fracture mechanics
- Neuroscience and Neuropharmacology Research
- Microfluidic and Capillary Electrophoresis Applications
Karolinska Institutet
2021-2025
KTH Royal Institute of Technology
2018-2025
Science for Life Laboratory
2022-2024
University of Leeds
2020
Earthquake Planning and Protection Organization
2020
London School of Economics and Political Science
2020
Abstract Cell-based models of the blood–brain barrier (BBB) are important for increasing knowledge BBB formation, degradation and brain exposure drug substances. Human preferred over animal because interspecies differences in structure function. However, access to human primary tissue is limited has shown degeneration functions vitro. induced pluripotent stem cells (iPSCs) can be used generate relevant cell types model with tissue. We generated a iPSC-derived that includes endothelial...
3D tissue models recapitulating human physiology are important for fundamental biomedical research, and they hold promise to become a new tool in drug development. An integrated defined microvasculature is necessary optimal cell functions. However, conventional bioprinting only allows the fabrication of hydrogel scaffolds containing vessel-like structures with large diameters (>100 µm) simple geometries. Recent developments laser photoablation enable generation this type structure higher...
Abstract The generation of astrocytes from human induced pluripotent stem cells has been hampered by either prolonged differentiation—spanning over two months—or shorter protocols that generate immature astrocytes, devoid salient mature astrocytic traits pivotal for central nervous system (CNS) modeling. We directed stable hiPSC-derived neuroepithelial to iPSC-derived Astrocytes (hiAstrocytes) with a high percentage star-shaped orchestrating an astrocytic-tuned culturing environment in 28...
Abstract Microphysiological systems mimic the in vivo cellular ensemble and microenvironment with goal of providing more human‐like models for biopharmaceutical research. In this study, first such model blood‐brain barrier (BBB‐on‐chip) featuring both isogenic human induced pluripotent stem cell (hiPSC)‐derived cells continuous integrity monitoring <2 min temporal resolution is reported. Its capabilities are showcased microphysiological study nitrosative stress antioxidant prophylaxis....
The breast cancer resistance protein (BCRP) is an important efflux transporter in the blood-brain barrier (BBB), protecting brain from a wide range of substances. In this study, we investigated if BCRP function affected by bisphenol A (BPA), high production volume chemical used common consumer products, as well F (BPF) and S (BPS), which are to substitute BPA. We employed transwell-based vitro cell model iPSC-derived microvascular endothelial cells, where was assessed measuring intracellular...
Background: Microglia and astrocytes have been implicated as central mediators of neuroinflammatory processes in several neurodegenerative diseases. However, their intricate crosstalk contributions to pathogenesis remain elusive, highlighting the need for innovative vitro approaches investigating glial interactions neuroinflammation. The aim this study was develop advanced human-based coculture models explore inflammatory roles microglia . Methods: We utilized human induced pluripotent stem...
Neurodegenerative diseases (NDs) are an accelerating global health problem. Nevertheless, the stronghold of brain- blood-brain barrier (BBB) prevents drug penetrance and dwindles effective treatments. Therefore, it is crucial to identify Trojan horse-like carriers that can effectively cross reach brain tissue. We have previously developed polyunsaturated fatty acids (PUFA)-based nanostructured lipid (NLC), namely DHAH-NLC. These modulated with BBB-permeating compounds such as chitosan (CS)...
Abstract The clinical translation of induced pluripotent stem cells (iPSCs) holds great potential for personalized therapeutics. However, one the main obstacles is that current workflow to generate iPSCs expensive, time‐consuming, and requires standardization. A simplified cost‐effective microfluidic approach presented reprogramming fibroblasts into their subsequent differentiation neural (NSCs). This method exploits microphysiological technology, providing a 100‐fold reduction in reagents...
Abstract Microphysiological systems mimic the in-vivo cellular ensemble and microenvironment with goal of providing more human-like models for biopharmaceutical research. We report first such model blood-brain barrier (BBB-on-chip) featuring both isogenic human induced pluripotent stem cell (hiPSC)-derived cells continuous integrity monitoring <2-minute temporal resolution. showcase its capabilities in microphysiological study nitrosative stress antioxidant prophylaxis. Relying on...
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Summary Generating astrocytes from induced pluripotent stem cells has been hampered by either prolonged differentiation -spanning over two months -or shorter protocols that generate immature astrocytes, devoid of salient inflammation-associated astrocytic traits pivotal for CNS neuropathological modeling. We directed human neural derived to commitment and maturity orchestrating an astrocytic-tuned culturing environment. In under 28 days, the generated express canonical mature markers,...
Microphysiological Systems In article number 2101785, Thomas E. Winkler, Anna Herland, and co-authors describe a microphysiological blood-brain barrier model that captures the multicellular interactions of iPS-derived cells. The integrated electrical sensors, facilitated by PDMS-free fabrication, allow for real-time monitoring how responds to oxidative stress antioxidant prophylaxis.