A5028815367- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Hematopoietic Stem Cell Transplantation
- Phase-change materials and chalcogenides
- Acute Myeloid Leukemia Research
- Proteoglycans and glycosaminoglycans research
- T-cell and B-cell Immunology
- Peptidase Inhibition and Analysis
- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- Liquid Crystal Research Advancements
- Immune Cell Function and Interaction
- Mesenchymal stem cell research
- Cancer Cells and Metastasis
- Children's Physical and Motor Development
- Chalcogenide Semiconductor Thin Films
- Pancreatic function and diabetes
- Child Development and Education
- Tea Polyphenols and Effects
- RNA and protein synthesis mechanisms
- Viral Infections and Vectors
- Drug Transport and Resistance Mechanisms
- Mercury impact and mitigation studies
Kibi International University
2021-2024
Tokyo University of Pharmacy and Life Sciences
2021
Tohoku University
1981-2017
Japan Women's University
2014
National Institute of Advanced Industrial Science and Technology
2002-2011
Keio University
2007-2010
Kindai University
1999-2009
Kindai University Sakai Hospital
2009
Saitama Medical University
2006
National Institute of Biomedical Innovation, Health and Nutrition
2006
Coordinated control of energy metabolism and glucose homeostasis requires communication between organs tissues. We identified a neuronal pathway that participates in the cross talk liver adipose tissue. By studying mouse model, we showed adenovirus-mediated expression peroxisome proliferator-activated receptor (PPAR)-g2 induces acute hepatic steatosis while markedly decreasing peripheral adiposity. These changes were accompanied by increased expenditure improved systemic insulin sensitivity....
The expression of Lewis histo-blood group antigen is determined by the type alpha(1,3/1,4) fucosyltransferase encoded in Fuc-TIII gene (Le gene) on chromosome 19. Weak hemagglutination reactions are often observed blood typing to cause false-negative reactions. level CA19-9 tumor marker, defined as sialylated Le(a) (sLe(a)), influenced phenotype. sLe(a) also considered play a role hematogenous metastasis cells. accurate genotyping would be, therefore, necessary for solving above questions....
Common molecular machineries between hematopoietic stem cell (HSC) maintenance and leukemia prevention have been highlighted. The tumor suppressor Fbxw7 (F-box WD-40 domain protein 7), a subunit of an SCF-type ubiquitin ligase complex, induces the degradation positive regulators cycle. We demonstrate that inactivation in cells causes premature depletion HSCs due to active cycling p53-dependent apoptosis. Interestingly, deletion also confers selective advantage with suppressed p53 function,...
The core 3 structure of the O-glycan, GlcNAcβ1–3GalNAcα1-serine/threonine, an important precursor in biosynthesis mucin-type glycoproteins, is synthesized by UDP-N-acetylglucosamine:GalNAc-peptide β1,3-N- acetylglucosaminyltransferase (β3Gn-T; synthase). restricted its occurrence to mucins from specific tissues such as stomach, small intestine, and colon. A partial sequence encoding a novel member human β3Gn-T family was found one data bases. We cloned complementary DNA this gene named it...
To date, 10 members of the UDP-<i>N</i>-acetyl-α-d-galactosamine:polypeptide<i>N</i>-acetylgalactosaminyltransferase (pp-GalNAc-T) family have been cloned and analyzed in human. In this study, we a novel human pp-GalNAc-T from an NT2 cell cDNA library, named it pp-GalNAc-T13. amino acid sequences, pp-GalNAc-T13 was highly homologous, showing 84.3% identity, to pp-GalNAc-T1. Real time PCR analysis revealed <i>pp-GalNAc-T13</i> be restrictively expressed brain present at very low or...
The Lewis histo-blood group system comprises two major antigens, a and b. b antigen is product of fucosyltransferases, the α(1,3/1,4)fucosyltransferase (Lewis enzyme; Fuc-TIII) encoded by gene an α(1,2)fucosyltransferase which not required for synthesis antigen. An enzyme responsible secreting ABH antigens into body secretions (secretor enzyme) also one α(1,2)fucosyltransferases. A candidate encoding secretor Sec2 was recently cloned Rouquier, S., Lowe, J. B., Kelly, R. J., Fertitta, A. L.,...
The amino acid sequence of Fuc‐TIX is very highly conserved between mouse and human. number non‐synonymous nucleotide substitutions the gene human was strikingly low, almost equivalent to that α‐actin gene. This indicates under a strong selective pressure preservation during evolution. (hFuc‐TIX) showed unique characteristics, i.e. hFuc‐TIX not activated by Mn 2+ Co , whereas hFuc‐TIV hFuc‐TVI were cations. transcripts abundantly expressed in brain stomach, interestingly detected spleen...
A new member of the UDP-N-acetylglucosamine:β-galactose β1,3-N-acetylglucosaminyltransferase (β3Gn-T) family having β3Gn-T motifs was cloned from rat and human cDNA libraries named β3Gn-T5 based on its position in a phylogenetic tree. We concluded that is most feasible candidate for lactotriaosylceramide (Lc3Cer) synthase, an important enzyme which plays key role synthesis lacto- or neolacto-series carbohydrate chains glycolipids. exhibited strong activity to transfer GlcNAc glycolipid...
Aiming to obtain a rewritable compact disc (CD-rewritable) with high performance at both CD-velocity (CD1X) and double (CD2X), the phase change optical Ag-In-Sb-Te system has been developed. In one-pass overwriting mode eight-to-fourteen modulation, carrier-to-noise ratio, wide power margin erase ratio have obtained velocities. As for stability, we acquired satisfactory result in read-out test of 10 6 cycles read 1.5 mW. We conclude that is suitable use CD1X CD2X. Furthermore, process also...
By a tblastn search with β1,4-galactosyltransferases as query sequences, we found an expressed sequence tag that showed similarity in β1,4-glycosyltransferase motifs. The full-length complementary DNA was obtained by method of 5′-rapid amplification ends. predicted open reading frame encodes typical type II membrane protein comprising 543 amino acids, the which highly homologous to chondroitin sulfate <i>N</i>-acetylgalactosaminyltransferase (CSGalNAcT-1), and designated this novel enzyme...
A phase change optical disk using a new quaternary system of Ag-In-Sb-Te as the active layer will be reported. With this disk, “complete erasure” recorded signal has been achieved. carrier-to-noise ratio 47 dB and an erase -47 were obtained in two-pass writing-erasing mode with linear velocity 7 m/s writing power 11 mW. In one-pass overwriting mode, greater than 40 was also completely erased. The peak bias for 10 mW 5 mW, respectively. facts that widths written marks are narrow no large...
Jacalin, a lectin from the jackfruit Artocarpus integrifolia, has been known as valuable tool for specific capturing of O-glycoproteins such mucins and IgA1. Though its sugar-binding preference T/Tn-antigens is well established, detailed specificity not elucidated. In this study, we prepared series mucin-type glycopeptides using human glycosyltransferases, that is, ST6GalNAc1, Core1Gal-T1 -T2, β3Gn-T6, Core2GnT1, investigated their binding to immobilized Jacalin by frontal affinity...
A novel member of the human UDP‐ N ‐acetyl‐ D ‐galactosamine:polypeptide ‐acetylgalactosaminyltransferase (pp‐GalNAc‐T) gene family was cloned as a homolog pp‐GalNAc‐T7, and designated pp‐GalNAc‐T10. pp‐GalNAc‐T10 transcript found in small intestine, stomach, pancreas, ovary, thyroid gland spleen. In polypeptide GalNAc‐transfer assay, recombinant transferred GalNAc onto panel mucin‐derived peptide substrates. Furthermore, demonstrated strong transferase activity with glycopeptide
We found a novel human glycosyltransferase gene carrying hypothetical β1,4-glycosyltransferase motif during BLAST search, and we cloned its full-length open reading frame by using the 5′-rapid amplification of cDNA ends method. It encodes type II transmembrane protein 999 amino acids with homology to chondroitin sulfate synthase in C-terminal region (GenBank™ accession number AB089940). Its putative orthologous was also mouse (accession AB114826). The truncated form enzyme expressed HEK293T...
The hinge region of human immunoglobulin A1 (*IgA1) possesses multiple O-glycans, which synthesis is initiated by the addition GalNAc to serine or threonine through activity UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferases (pp-GalNAc-Ts). We found that six pp-GalNAc-Ts, pp-GalNAc-T1, -T2, -T3, -T4, -T6, and -T9, were expressed in B cells, IgA-bearing NCI-H929 IgA myeloma cells. pp-GalNAc-T activities these enzymes for a synthetic peptide, has nine possible...
We analyzed the substrate specificity of six human α1,3‐fucosyltransferases (α1,3FUTs) for 2‐aminobenzamide (2AB)‐labelled polylactosamine acceptor, Galβ1‐4GlcNAcβ1‐3Galβ1‐4GlcNAcβ1‐3Galβ1‐4GlcNAc‐2AB (3LN‐2AB). FUT9 preferentially fucosylated distal GlcNAc residue chain while other four α1,3FUT members, FUT3, FUT4, FUT5 and FUT6, inner residue. This indicated that exhibits more efficient activity synthesis Lewis x carbohydrate epitope (Le ; CD15; stage‐specific embryonal antigen‐1...