A5101464019- Mesenchymal stem cell research
- Angiogenesis and VEGF in Cancer
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Bone and Dental Protein Studies
- Congenital heart defects research
- Pluripotent Stem Cells Research
- Cancer, Hypoxia, and Metabolism
- Zebrafish Biomedical Research Applications
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Hematopoietic Stem Cell Transplantation
- Galectins and Cancer Biology
- Chemokine receptors and signaling
- Electrospun Nanofibers in Biomedical Applications
- Connective tissue disorders research
- Hematological disorders and diagnostics
- Bird parasitology and diseases
- Extracellular vesicles in disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Animal Virus Infections Studies
- Neonatal Respiratory Health Research
- Xenotransplantation and immune response
- Pregnancy and preeclampsia studies
- Phagocytosis and Immune Regulation
Central South University
2008-2021
National Engineering Research Center of Human Stem Cells
2007-2021
Benaroya Research Institute
2013
The First Hospital of Changsha
2007
National Institute of Advanced Industrial Science and Technology
2002-2004
To date, 10 members of the UDP-<i>N</i>-acetyl-α-d-galactosamine:polypeptide<i>N</i>-acetylgalactosaminyltransferase (pp-GalNAc-T) family have been cloned and analyzed in human. In this study, we a novel human pp-GalNAc-T from an NT2 cell cDNA library, named it pp-GalNAc-T13. amino acid sequences, pp-GalNAc-T13 was highly homologous, showing 84.3% identity, to pp-GalNAc-T1. Real time PCR analysis revealed <i>pp-GalNAc-T13</i> be restrictively expressed brain present at very low or...
Angiogenesis is a complicated process in which perivascular cells play important roles. Multipotent mesenchymal stem/stromal (MSCs) from distinct tissues have been proved to be proangiogenic and share functional properties gene expression profiles with cells. However, different derived MSCs may exhibit potential for clinical applications. Accordingly, comparative studies on are essential. Here, we characterized adipose (ADSCs), umbilical cord (UCMSCs), endometrium (EMSCs) terms of the...
Jacalin, a lectin from the jackfruit Artocarpus integrifolia, has been known as valuable tool for specific capturing of O-glycoproteins such mucins and IgA1. Though its sugar-binding preference T/Tn-antigens is well established, detailed specificity not elucidated. In this study, we prepared series mucin-type glycopeptides using human glycosyltransferases, that is, ST6GalNAc1, Core1Gal-T1 -T2, β3Gn-T6, Core2GnT1, investigated their binding to immobilized Jacalin by frontal affinity...
A novel member of the human UDP‐ N ‐acetyl‐ D ‐galactosamine:polypeptide ‐acetylgalactosaminyltransferase (pp‐GalNAc‐T) gene family was cloned as a homolog pp‐GalNAc‐T7, and designated pp‐GalNAc‐T10. pp‐GalNAc‐T10 transcript found in small intestine, stomach, pancreas, ovary, thyroid gland spleen. In polypeptide GalNAc‐transfer assay, recombinant transferred GalNAc onto panel mucin‐derived peptide substrates. Furthermore, demonstrated strong transferase activity with glycopeptide
We cloned in silico a novel human UDP‐GalNAc:polypeptide N ‐acetylgalactosaminyltransferase (pp‐GalNAc‐T), pp‐GalNAc‐T12. The deduced amino acid sequence of pp‐GalNAc‐T12 contains all conserved motifs pp‐GalNAc‐T family proteins. Quantitative real time polymerase chain reaction analysis revealed that the transcript was expressed mainly digestive organs such as stomach, small intestine and colon. recombinant transferred GalNAc to mucin‐derived peptides Muc1a, Muc5AC, EA2 GalNAc‐Muc5AC...
We have cloned, expressed and characterized a novel member of the human UDP‐GalNAc:polypeptide N ‐acetylgalactosaminyltransferase (pp‐GalNAc‐T) family, pp‐GalNAc‐T15. The pp‐GalNAc‐T15 transcript was ubiquitously in tissues. Recombinant transferred ‐acetylgalactosamine (GalNAc) toward panel mucin‐derived peptide substrates vitro. Although showed significantly less catalytic activity than pp‐GalNAc‐T2, T15 up to seven GalNAcs Muc5AC peptide, while T2 five GalNAcs. These results clearly...
Summary Cumulative evidences have revealed that endothelial progenitor cell (EPC) transplantation can promote the neovascularization in ischemic tissue, but mechanism of EPCs homing to site ischemia is poorly understood. In this study, investigate human umbilical cord blood-derived high proliferative potential-endothelial cells (HPP-EPCs) tissue we evaluated expression lymphocyte function-associated antigen-1 (LFA-1, or CD11a/CD18) and very late antigen-4 (VLA-4, CD49d/CD29) changes level...
This study was designed to investigate whether oxidized low-density lipoprotein (oxLDL) affects the survival and activity of endothelial progenitor cell (EPC) mediated by p38 mitogen-activated protein kinase (MAPK). EPCs were isolated from human peripheral blood in growth medium-2. Incubation with oxLDL at 100 μg/mL decreased EPC number. Treated resulted increase apoptosis decrease proliferation. Treatment a significantly reduced migratory rate adhesion fibronectin. impaired vitro...
Secreted protein, acidic and rich in cysteine (SPARC) has been described as a counteradhesive matricellular protein with diversity of biological functions associated morphogenesis, remodeling, cellular migration, proliferation. We have produced mouse SPARC FLAG-tag at the N-terminus (Flag-SPARC, FSP) Bac-to-Bac baculoviral expression system. After affinity purification, this procedure yields high purity, an electrophoretic mobility ∼44 kDa under reducing conditions, ∼38–39 non-reducing...
Here we report a critically ill patient who was cured of SARS-CoV-2 infection in Changsha, China. A 66-year-old Chinese woman, with no significant past medical history, developed severe pneumonia-like symptoms and later diagnosed as COVID-19 pneumonia. Within 2 months hospitalization, the deteriorated to ARDS including pulmonary edema SIRS septic shock. When treatment schemes such antibiotics plus corticosteroids showed diminished therapeutic value, hUCMSC therapy compassionately prescribed...
Abstract We have examined the effect of mouse bone marrow endothelial cell‐conditioned medium (mEC‐CM) on hematopoietic and differentiation embryonic stem cells (mESCs). mEC‐CM can efficiently promote mESCs into Flk + colony‐forming cells. proved to be as potent a cytokine cocktail comprised VEGF, bFGF, IGF EGF. After inducing with mEC‐CM, cobblestone‐like were mechanically selected identified which had ability incorporate DiI‐Ac‐LDL. DiI‐Ac‐LDL‐positive endothelial‐like due their expression...
Human umbilical cord blood-derived endothelial progenitor cells (EPCs) have been proven to contribute post-natal angiogenesis, and applied in various models of ischemia. However, date, the best our knowledge, there is no available data on angiogenic properties EPCs during process vitro expansion. In this study, we expanded obtain at different passages, analyzed their cellular ability. expansion, changes were observed cell cobblestone-like morphology, apoptotic rate telomere length....
Macrophage stimulating protein (MSP) is produced by human bone marrow endothelial cells. In this study, we sought to observe its effects on inducing the expansion of early hematopoietic progenitor cells which were cultured in a liquid culture system presence combination stem cell factor (SCF), interleukin 3 (IL-3), 6 (IL-6), granulocyte macrophage-colony (GM-CSF), erythropoietin (EPO) (Cys) and MSP or Cys conditioned medium (EC-CM).Human CD34(+) separated for days. Granulocyte-macrophage...
To compare the ability between bone marrow-derived mesenchymal stem cells (MSCs) (BM-MSCs) and adipose-derived MSCs (AD-MSCs) or umbilical cord-derived (UC-MSCs) on promotion of vessels formation stabilization relevant to functions EPCs. Methods: In vitro, co-culture blood vessel test was performed angiogenic BM-MSCs, AD-MSCs UC-MSCs. vivo, assay dependent basement membrane matrix Matrigel immunohistochemistry were BM-MSCs Results: The lengths dots vascular structures formed by EPCs layer...
The members of the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T) family, which transfer GalNAc to polypeptide serine and threonine residues, initiate mucin-type O-linked glycosylation. There are at least 13 functionally characterized this family in humans, but no studies have been reported pp-GalNAc-T isoforms hematopoietic cells. We isolated purified CD34+ cells from adult bone marrow by magnetic cell sorting induced them differentiate into megakaryocytic lineage...