The present study was undertaken to investigate the role of angiotensin II (Ang II) in ovulation and ovarian steroidogenesis prostaglandin (PG) production via Ang receptors rabbit ovaries. In vitro perfused ovaries, PD123319, a selective nonpeptide antagonist for AT2 receptors, reduced II-induced dose-dependent manner, whereas CV-11974, AT1 receptor, did not affect ovulation. also significantly stimulated meiotic maturation ovulated ova follicular oocytes absence gonadotropin. addition...

The present study was undertaken to assess the role of ovarian renin-angiotensin system (RAS) in preovulatory cascade induced by gonadotropin exposure. In vitro perfused rabbit ovaries, exposure human chorionic (hCG) enhanced secretion rate angiotensin II (Ang II) within 1 h. reached maximal levels at 6 h and then declined thereafter. intrafollicular Ang content renin-like activity were also significantly increased 2 4 after hCG, compared with control ovaries medium alone. level hCG exceeded...

To investigate the possible direct involvement of angiotensin II (Ang II) in ovulation and oocyte maturation, Ang at 100 or 10 micrograms was administered 2-h intervals in-vitro perfused rabbit ovaries. The addition perfusate induced vitro absence gonadotropin, while did not occur any contralateral control However, ovulatory efficiency II-treated ovaries significantly lower than hCG-treated stimulated meiotic maturation ovulated ova follicular oocytes. Concomitant specific receptor...

The present study was undertaken to investigate the role of exogenous and endogenous angiotensin II (Ang II) in ovarian steroidogenesis production prostaglandin (PG) vitro perfused rabbit ovaries. addition 100 or 10 micrograms Ang at 2-h intervals perfusate did not stimulate progesterone production, but significantly stimulated estradiol (E2) by When specific antagonist II, saralasin 2 x 10(-6) M, added 30 min before onset administration, II-stimulated E2 blocked. also both PGE2 PGF2 alpha...

The present study was undertaken to investigate the effects of GH on follicular growth, oocyte maturation, ovulation, and production insulin-like growth factor-I (IGF-I) in vitro perfused rabbit ovaries. Ovulation did not occur any ovaries with at a concentration 1, 10, 100, or 200 ng/ml, but addition perfusate increased follicle diameter dose-dependent manner. IGF-I by medium alone very low throughout perfusion period. 100 ng/ml significantly ovarian 4, 6, 8, 12 h compared contralateral...

Lung cancer is frequently complicated by interstitial lung disease (ILD). Treatment protocols for patients with ILD have not been established; surgery, chemotherapy, and radiotherapy can all cause acute exacerbation of ILD. This study evaluated the toxicity efficacy carbon ion (CIRT) in non-small cell (NSCLC) ILD.Between June 2004 November 2014, 29 diagnosed NSCLC were treated CIRT. No patient was eligible curative surgery or conventional secondary to Owing prior symptomology, radiation...

Summary. The involvement of cyclic adenosine monophosphate (cAMP) in mammalian oocyte maturation was assessed using cultures rabbit cumulus–oocyte complexes and perfused ovaries. Rabbit were cultured Brackett's medium with or without forskolin at 10−4, 10−5 10−6 mol l−1 for 3–6 h. At 3 4 h spontaneous meiotic significantly (P < 0·05) inhibited by 10−4 l−1. With prolonged incubation, progressed despite exposure to forskolin. In the second experiment ovaries 12 (10−4, 10−6mol l−1) alone....

Carbon-ion radiation therapy (CIRT) for advanced non-small-cell lung cancer (NSCLC) has not been well studied to date. This paper aimed analyze a retrospective multicenter survey detecting problems with the use of CIRT Stage II and III NSCLC (7th UICC TNM Staging System). Inclusion was restricted patients who received from November 2003 December 2014. We gathered data three operating centers on July 2015. Patients radiotherapy history, cancers other than cancer, those receiving palliative...

Intrathoracic recurrence after carbon‐ion radiotherapy for primary or metastatic lung tumors remains a major cause of cancer‐related deaths. However, treatment options are limited. Herein, we report on the toxicity and efficacy re‐irradiation with locoregionally recurrent, metastatic, secondary tumors. Data 95 patients prior intrathoracic who were treated at our institution between 2006 2016 retrospectively analyzed. Seventy‐three (76.8%) had 22 (23.2%) The median dose initial was 52.8 Gy...

Carbon-ion radiotherapy (CIRT) is a promising treatment for locally advanced non-small-cell lung cancer, especially patients with inoperable cancer. Although the incidence of CIRT-induced radiation pneumonitis (RP) ≥ grade 2 ranges from 2.5 to 9.9%, association between RP and dosimetric parameters not clear. Herein, we identified prognostic factors associated symptomatic after CIRT Clinical results 65 treated 2000 2015 at National Institute Radiological Sciences were retrospectively...

The present study was undertaken to assess the role of alterations intraoocyte cAMP concentrations in meiotic maturation isolated and follicle-enclosed oocytes. In oocyte culture, intracellular content denuded oocytes declined within 15 min incubation, whereas cumulus-enclosed did not change substantially for 1.5 h then abruptly. Commitment preceded by reduced cAMP. Forskolin inhibited spontaneous a dose-dependent manner. However, this inhibition attenuated as duration incubation increased....

Abstract Serine-threonine kinase 38 (STK38) is a member of the protein A (PKA)/PKG/PKC-family implicated in regulation cell division and morphogenesis. However, molecular mechanisms underlying STK38 stability remain largely unknown. Here, we show that treatment cells with either heat or calcium ionophore A23187 induced degradation. The calpain inhibitor calpeptin suppressed hyperthermia-induced degradation appearance A23187-induced cleaved form STK38. An vitro cleavage assay was then used to...

Skin toxicity caused by radiotherapy has been visually classified into discrete grades. The present study proposes an objective and continuous assessment method of skin erythema in digital images taken under arbitrary lighting conditions, which is the case for most clinical environments. purpose this paper to show feasibility proposed method.Clinical data were gathered from six patients who received carbon beam therapy lung cancer. condition was recorded using ordinary compact camera unfixed...

Carbon-ion beam scanning has not previously been used for moving tumor treatments. We have commenced respiratory-gated carbon-ion radiotherapy (CIRT) in the thoracic and abdominal regions under free-breathing conditions as a clinical trial. This study aimed to investigate this treatment lungs comparison with passive scattering CIRT. Five patients had tumors treated scanned beams using respiratory gating. analyzed actual treatments calculated plans based on same planning CT. evaluated size...

The present study was undertaken to assess the effects of prolactin (PRL) on gonadotropin-induced plasmin generation in vitro-perfused rabbit ovary. ovarian activity determined by measuring bound its major inhibitor, α2-plasmin inhibitor (α2PI-Plm). In first experiment, exposure hCG enhanced α2PI-Plm from 1.2 ± 0.3 ng/min/ovary unstimulated ovaries 2.9 ng within 2 h. concentration reached a maximum at 4 h and then declined. A second peak occurred 8 after administration; however, without very...

This is a first paper to report on artificial ascites infusion via vaginal wall for pelvic interstitial brachytherapy. Artificial commonly used treating liver tumors, with radiofrequency ablation and percutaneous through the abdominal brachytherapy has been previously reported by our group. However, trans-abdominal needle approach under ultrasound guidance unreliable due poor visualization resulting in fluid injection into or mesenterium rate of successful was low. Target tumor cuff usually...

The emergence of an aging society and technological advances have made radiotherapy, especially stereotactic body radiotherapy (SBRT), a common alternative to surgery for elderly patients with early stage non-small-cell lung cancer (NSCLC). Carbon-ion (CIRT) is also attractive treatment option potentially lower toxicity comorbidities. We compared the clinical outcomes two modalities using Japanese multicenter data. SBRT (n = 420) single-fraction CIRT 70) data I NSCLC from 20 centers were...

PROBLEM: This study was undertaken to assess whether growth hormone (GH) can stimulate follicle and ovarian steroidogenesis via putative GH receptors. METHOD: In vitro perfused rabbit ovary. RESULTS: Ovulation occurred in neither the control ovaries nor experimental treated with 100 ng/ml of GH, whereas all exposed 50 IU human chorionic gonadotropin (hCG) ovulated. The addition perfusate significantly stimulated absence gonadotropin. percent change diameter GH‐treated did not differ from...