A5081686854- Medical and Biological Sciences
- Medical Imaging Techniques and Applications
- Surgical Sutures and Adhesives
- RNA Research and Splicing
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Electrospun Nanofibers in Biomedical Applications
- Genomics and Chromatin Dynamics
- Glioma Diagnosis and Treatment
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Gene expression and cancer classification
- Pelvic and Acetabular Injuries
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Abdominal Trauma and Injuries
- Trauma and Emergency Care Studies
- Cardiac tumors and thrombi
- nanoparticles nucleation surface interactions
- Molecular Biology Techniques and Applications
- Pulmonary Hypertension Research and Treatments
- Machine Learning in Materials Science
- Hemostasis and retained surgical items
- Genetic Syndromes and Imprinting
- Pregnancy-related medical research
Gifu University
2015-2025
Saitama Medical University
2021-2025
Gifu University Hospital
2021-2025
NTT Basic Research Laboratories
2025
Tokyo Institute of Technology
2024
National Institute for Materials Science
2017-2021
University of Tsukuba
1999-2021
Ehime University Hospital
2014-2021
Matsuyama Red Cross Hospital
2011-2019
Ehime University
2014-2017
Antisense transcription (transcription from the opposite strand to a protein-coding or sense strand) has been ascribed roles in gene regulation involving degradation of corresponding transcripts (RNA interference), as well silencing at chromatin level. Global transcriptome analysis provides evidence that large proportion genome can produce both strands, and antisense commonly link neighboring "genes" complex loci into chains linked transcriptional units. Expression profiling reveals frequent...
Although it is generally accepted that cellular differentiation requires changes to transcriptional networks, dynamic regulation of promoters and enhancers at specific sets genes has not been previously studied en masse. Exploiting the fact active are transcribed, we simultaneously measured their activity in 19 human 14 mouse time courses covering a wide range cell types biological stimuli. Enhancer RNAs, then messenger RNAs encoding transcription factors, dominated earliest responses....
We have systematically characterized gene expression patterns in 49 adult and embryonic mouse tissues by using cDNA microarrays with 18,816 cDNAs. Cluster analysis defined sets of genes that were expressed ubiquitously or similar groups such as digestive organs muscle. Clustering profiles was observed brain, postnatal cerebellum, olfactory bulb, reflecting similarities neurogenesis remodeling. Finally, clustering coding for known enzymes into 78 metabolic pathways revealed a surprising...
Candidate imprinted transcriptional units in the mouse genome were identified systematically from 27,663 FANTOM2 full-length cDNA clones by expression profiling. Large-scale microarrays used to detect differential dependent upon chromosomal parent of origin comparing mRNA levels total tissue 9.5 dpc parthenogenote and androgenote embryos. Of transcripts, 2114 as candidates on basis array data. these, 39 mapped known regions genome, 56 considered nonprotein-coding RNAs, 159 natural antisense...
The aim of this study was the development an innovative biocompatible sealant composed Alaska pollock-derived gelatin partially modified with a dodecyl group (C12-ApGltn) and poly(ethylene glycol)-based crosslinker, pentaerythritol glycol) ether tetrasuccinimidyl glutarate. burst strength developed sealants measured using porcine aorta rat lungs. maximum C12-ApGltn-based against 4-fold higher than that original ApGltn (Org-ApGltn)-based sealant. No significant increase in observed between...
Fabrication of a biohybrid actuator requires muscle cells anisotropically aligned in line, curve, or combination lines and curves (similar to the microstructure living tissue) replicate lifelike movements, addition considering arrangement skeletal structure muscular with anisotropic straight patterns. Here, we report UV laser-processed for freely directing cellular alignment engineer composed poly(styrene-block-butadiene-block-styrene triblock copolymer) (SBS) thin film tailor-made...
We present a simple method for bonding an elastomer substrates using stretchable, low-molecular-weight adhesive. By mixing 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC) and gallic acid on aminated surface, EDC is converted into...
Background Small bowel injuries are rare in trauma. Diagnosing these is difficult owing to the lack of clear signs, and delays diagnosis might increase complications mortality. Trauma can damage vascular endothelial glycocalyx, with syndecan-1 emerging as a marker injury. Here, we describe two cases small injury due traffic motor vehicle crash Case presentation The patients, one their 40s teenaged, were transported our hospital after crash. Both patients wearing seat belts at time crash,...
Peripheral nerve stimulation using implantable electrodes has gained significant attention as a potential treatment for drug-resistant neurological disorders. Developing deformable thin-film electrode devices is essential to minimize damage when interfacing with fine fibers. Here, we demonstrate self-folding graphene-based peripheral stimulation. The films were patterned holes and slits control the folding direction, enabling thin film wrap around fibers while allowing current application...
Abstract This study aims to investigate the function of miR-424 and miR-503, identified as putative regulatory miRNAs FOXO1 , a key factor for decidualization. The expression both miR-503 in human endometrial stromal cells (HESCs) were measured before after Then, HESCs transfected with Quantitative reverse transcription PCR, actin staining analysis, migration assay, fluorescence immunostaining, luciferase assay performed. MiR-424 was decreased Overexpression inhibited major decidual maker...
Abstract Background Rhabdomyolysis is a clinical syndrome resulting from skeletal muscle damage and the release of its breakdown products into bloodstream. It can range asymptomatic cases to severe conditions such as acute kidney injury. Although myoglobin (molecular weight 17.2 kDa) blood associated with progression injury, there no established method prevent it. Here, we report case hypermyoglobinemia due rhabdomyolysis caused by reperfusion injury following limb ischemia, where early...
The number of known mRNA transcripts in the mouse has been greatly expanded by RIKEN Mouse Gene Encyclopedia project. Validation their reproducible expression a tissue is an important contribution to study functional genomics. In this report, we determine profile 57,931 clones on 20 tissues using cDNA microarrays. Of these clones, 22,928 correspond FANTOM2 clone set. set represents 20,234 transcriptional units (TUs) out 33,409 TUs We identified 7206 separate that satisfied stringent criteria...
Over 4 million single nucleotide polymorphisms (SNPs) are currently reported to exist within the human genome. Only a small fraction of these SNPs alter gene function or expression, and therefore might be associated with cell phenotype. These functional consequently important in understanding health. Information related candidate disease genes is critical for cost effective genetic association studies, which attempt understand genetics complex diseases like diabetes, Alzheimer's, etc. Robust...
Objectives The secretory enzyme lysyl oxidase like 2 (LOXL2) is speculated to contribute tumor progression through its functions in the remodeling of extracellular matrix and epithelial‐mesenchymal transition. We previously identified elevated expression LOXL2 metastatic human head neck squamous cell carcinoma (HNSCC) cells a mouse lymph node metastases model. Here we performed case series study examining levels serum from HNSCC patients evaluate whether worth evaluation large cohort study....
We have developed a method for high-efficiency window separation of cDNA display by increasing the specificity priming in reverse transcription. In conventional method, two-base anchored oligo(dT) primers (5′dT16VN3′, where N is any base and V G, A or C) are used to make windows transcripts. However, transcriptase often extends misprimed oligonucleotides. To avoid mispriming from dT16VN primers, we two new technologies. One higher temperature with thermoactivated disaccharide trehalose. The...
Tissue injury and hemorrhage induced by trauma lead to degradation of the endothelial glycocalyx, causing syndecan-1 (SDC-1) be shed into blood. In this study, we investigated whether serum SDC-1 is useful for evaluating severity in patients. A single-center, retrospective, observational study was conducted at Gifu University Hospital. Patients transported emergency room subsequently admitted intensive care unit from January 2019 December 2021 were enrolled. linear regression model...