A5058445149- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Herpesvirus Infections and Treatments
- RNA and protein synthesis mechanisms
- Animal Virus Infections Studies
- Cytomegalovirus and herpesvirus research
- Bacteriophages and microbial interactions
- Cancer-related Molecular Pathways
- Glycosylation and Glycoproteins Research
- Cancer therapeutics and mechanisms
- Ubiquitin and proteasome pathways
University of Padua
2021-2025
i-Motifs (iMs) are four-stranded DNA structures that form at cytosine (C)-rich sequences in acidic conditions vitro. Their formation cells is still under debate. We performed CUT&Tag sequencing using the anti-iM antibody iMab and showed iMs within human genome live cells. mapped two cell lines recovered C-rich were confirmed to fold into found mainly present actively transcribing gene promoters, open chromatin regions, they overlap with R-loops, their abundance distribution specific each...
Understanding the mechanisms of carcinogenesis is essential to combat cancer. The search for alternative targets anticancer therapy has gained interest, particularly when focused on upstream pathways. This strategy relevant encoded target proteins are known - or believed be "undruggable", as been reported B-MYB oncogene. gene, which regulates survival and cell cycle regulation, overexpressed in cancer correlates with an unfavorable prognosis. In this study, we identification i-motif (iM)...
The herpes simplex virus 1 (HSV-1) genome is extremely rich in guanine tracts that fold into G-quadruplexes (G4s), nucleic acid secondary structures implicated key biological functions. Viral G4s were visualized HSV-1 infected cells, with massive cycle-dependent G4-formation peaking during viral DNA replication. Small molecules specifically interact have been shown to inhibit We here investigated the antiviral activity of TMPyP4, a porphyrin known G4s. analogue TMPyP2, lower G4 affinity, was...
i-Motifs (iMs) are quadruplex nucleic acid conformations that form in cytosine-rich regions. Because of their acidic pH dependence, iMs were thought to only vitro. The recent development an iM-selective antibody, iMab, has allowed iM detection cells, which revealed presence at gene promoters and cell cycle dependence. However, evidence emerged appeared suggest iMab recognizes C-rich sequences regardless conformation. To further investigate the selectivity we examined binding sequences, using...
Well-differentiated/dedifferentiated liposarcomas (WD/DDLPSs) account for ∼60% of all liposarcomas. They have a poor prognosis due to limited therapeutic options. WD/DDLPSs are characterized by aberrant expression mouse double minute 2 (MDM2), which forms G-quadruplexes (G4s) in its promoter. Here, we investigated the possibility targeting with small molecules against MDM2 G4s. Among tested, naphthalene diimide derivative QN-302 significantly impaired WD/DDLPS cell growth and activity...
ABSTRACT i-Motifs are quadruplex nucleic acid conformations that form in cytosine-rich regions. Because of their acidic pH dependence, iMs were thought to only vitro . The recent development an iM-selective antibody, iMab, has allowed iM detection cells, which revealed presence at gene promoters and cell cycle dependence. However, recently evidence emerged seemed suggest iMab recognizes C-rich sequences regardless conformation. To further investigate the selectivity we examined binding...
<title>Abstract</title> <italic>Mycobacterium tuberculosis (Mtb)</italic>, the causative agent of tuberculosis, remains a global health threat due to increasing drug resistance and high mortality rates. To combat effectively, novel therapeutic targets are urgently needed. G-quadruplexes (G4s) represent promising candidates for this purpose. In study, we successfully applied cleavage under tagmentation (CUT&Tag) technique first time in bacteria, mapping G4 landscape <italic>Mtb</italic>...
The DNA secondary structures that deviate from the classic Watson and Crick base pairing are increasingly being reported to form transiently in cell regulate specific cellular mechanisms. Human viruses parasites have evolved mechanisms shared with host support their own replication spreading. Contrary human cells, display a diverse array of nucleic acid types, which include or RNA single-stranded double-stranded conformations. This heterogeneity improves possible occurrence non-canonical...