A5028639180- Bone and Dental Protein Studies
- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- Immune cells in cancer
- Prostate Cancer Treatment and Research
- S100 Proteins and Annexins
- Ferroptosis and cancer prognosis
- Cancer, Lipids, and Metabolism
- Cancer-related gene regulation
- Vitamin D Research Studies
- Cancer, Stress, Anesthesia, and Immune Response
- Fibroblast Growth Factor Research
- Breast Cancer Treatment Studies
- Gene expression and cancer classification
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- COVID-19 Impact on Reproduction
Fondazione IRCCS Istituto Nazionale dei Tumori
2023-2024
Breast cancer is the most common type of in women worldwide, with luminal subtype being widespread. Although characterized by better prognosis compared other subtypes, breast still considered a threatening disease due to therapy resistance, which occurs via both cell- and non-cell-autonomous mechanisms. Jumonji domain-containing 6, arginine demethylase lysine hydroxylase (JMJD6) endowed negative prognostic value and, its epigenetic activity, it known regulate many intrinsic cell pathways. So...
Neuroendocrine prostate cancer (NEPC) is an aggressive form of that emerges as tumors become resistant to hormone therapies or, rarely, arises de novo in treatment-naïve patients. The urgent need for effective against NEPC hampered by the limited knowledge biology governing this lethal disease. Based on our prior observations transgenic adenocarcinoma mouse (TRAMP) spontaneous model, which genetic depletion either mast cells (MC) or matricellular protein osteopontin (OPN) increases...
<div>Abstract<p>Neuroendocrine prostate cancer (NEPC) is an aggressive form of that emerges as tumors become resistant to hormone therapies or, rarely, arises <i>de novo</i> in treatment-naïve patients. The urgent need for effective against NEPC hampered by the limited knowledge biology governing this lethal disease. Based on our prior observations transgenic adenocarcinoma mouse (TRAMP) spontaneous model, which genetic depletion either mast cells (MC) or...
<div>Abstract<p>Neuroendocrine prostate cancer (NEPC) is an aggressive form of that emerges as tumors become resistant to hormone therapies or, rarely, arises <i>de novo</i> in treatment-naïve patients. The urgent need for effective against NEPC hampered by the limited knowledge biology governing this lethal disease. Based on our prior observations transgenic adenocarcinoma mouse (TRAMP) spontaneous model, which genetic depletion either mast cells (MC) or...
<p>Supplementary Figure S7. Flow cytometry characterization of TNFa receptors and production. A. List cytokines chemokines tested through a multiplex immunoassay (Procarta-plex by Thermofisher) in the supernatants ST4787 or T23 cells cultured either alone presence WT, OPN-/-, MyD88-/- MCs. B.-C. evaluation TNFRs (CD120a CD120b) on adenocarcinoma (T23 murine, 22Rv1 human) NEPC (TC566 TC411K LASCPC-01 cells. D. Gating strategy applied for intracellular detection MCs (CD49f-CD45+) flow...
<p>Supplementary Figure S9. Expression and silencing of putative TLR ligands in NEPC cells. A. Venn diagrams show a list 29 genes (List 3; Supplementary Table S3) extrapolated from the intersection between found up-regulated TRAMP-derived incipient (a data set generated this study, GSE242811; List 1; surface TLR2/TLR4 identified ligand-receptor pairs repository (LewisLabUCSD, ref. (Armingol et al., 2021) 2; S3). B. Real Time-PCR for Cd14, Sdc1, Hspa2 Anxa2 on lysates T23 ST4787...
<p>Supplementary Figure S1. Flow cytometry evaluation of murine MC purity and maturation in vitro. A. Gating strategy used to evaluate the WT, OPN-/-, MyD88-/- TNFa-/-MCs by flow cytometry. Mature population is identified as c-Kit+FceRI+.</p>
<p>Supplementary Figure S5. Expression of OPN in MC/9 cells and effect on T23 cell proliferation. A. Separate channels immunofluorescence for DAPI (cyan), (red) WGA (blue) MC/9, MC/9-CTR, MC/9-OPNf, MC/9-iOPN showed Fig. 2E. B. Quantification A as percentage positive cells. C. Murine adenocarcinoma (T23) (50.000/well) were cultured either alone or with MC/9-OPNf (tumor cell:MC ratio 1:1). After 4 days the growth rate cancer was evaluated through trypan blue count. Cancer could be...
<p>Supplementary Figure S9. Expression and silencing of putative TLR ligands in NEPC cells. A. Venn diagrams show a list 29 genes (List 3; Supplementary Table S3) extrapolated from the intersection between found up-regulated TRAMP-derived incipient (a data set generated this study, GSE242811; List 1; surface TLR2/TLR4 identified ligand-receptor pairs repository (LewisLabUCSD, ref. (Armingol et al., 2021) 2; S3). B. Real Time-PCR for Cd14, Sdc1, Hspa2 Anxa2 on lysates T23 ST4787...
<p>Supplementary Figure S8. Hematoxylin and eosin staining in TRAMP mice. A. serial slides of tumors reported Fig. 5A, showing an untreated mouse with adenocarcinoma (ADENO) a subjected to surgical castration focal t-NEPC area.</p>
<p>Supplementary Figure S1. Flow cytometry evaluation of murine MC purity and maturation in vitro. A. Gating strategy used to evaluate the WT, OPN-/-, MyD88-/- TNFa-/-MCs by flow cytometry. Mature population is identified as c-Kit+FceRI+.</p>
<p>Supplementary Figure S5. Expression of OPN in MC/9 cells and effect on T23 cell proliferation. A. Separate channels immunofluorescence for DAPI (cyan), (red) WGA (blue) MC/9, MC/9-CTR, MC/9-OPNf, MC/9-iOPN showed Fig. 2E. B. Quantification A as percentage positive cells. C. Murine adenocarcinoma (T23) (50.000/well) were cultured either alone or with MC/9-OPNf (tumor cell:MC ratio 1:1). After 4 days the growth rate cancer was evaluated through trypan blue count. Cancer could be...
<p>Supplementary Figure S7. Flow cytometry characterization of TNFa receptors and production. A. List cytokines chemokines tested through a multiplex immunoassay (Procarta-plex by Thermofisher) in the supernatants ST4787 or T23 cells cultured either alone presence WT, OPN-/-, MyD88-/- MCs. B.-C. evaluation TNFRs (CD120a CD120b) on adenocarcinoma (T23 murine, 22Rv1 human) NEPC (TC566 TC411K LASCPC-01 cells. D. Gating strategy applied for intracellular detection MCs (CD49f-CD45+) flow...
<p>Supplementary Figure S3. Toluidine blue for detection of MCs in mice. A. MC count reported as number MCs/tumor area (cm2) tumor samples from TRAMP mice with prostate intraepithelial neoplasia or adenocarcinoma (PIN/ADENO, <i>n</i>= 12), NEPC (<i>n</i>= 3), well prostatectomies untreated patients Gleason Score 7 10), 8 (<i>n</i>=10), showing evidence 4). The histogram depict mean ± SD biological replicates (represented by dots). B. Upper panels:...
<p>Supplementary Figure S3. Toluidine blue for detection of MCs in mice. A. MC count reported as number MCs/tumor area (cm2) tumor samples from TRAMP mice with prostate intraepithelial neoplasia or adenocarcinoma (PIN/ADENO, <i>n</i>= 12), NEPC (<i>n</i>= 3), well prostatectomies untreated patients Gleason Score 7 10), 8 (<i>n</i>=10), showing evidence 4). The histogram depict mean ± SD biological replicates (represented by dots). B. Upper panels:...
<p>Supplementary Figure S6. Quantification of western blot. A. blot reported in Fig. 3G. Western blots were validated twice. All histograms depict mean ± SD biological replicates (represented by dots). One-way ANOVA followed Tukey’s multiple comparison test was used for the analysis significance between samples. <i>P</i>-values are as: *, <i>P</i> < 0.05; **, <0.01; ***, <0.001; ****, <0.0001. Where <i>P</i>-value is not indicated, groups...
<p>Supplementary Figure S4. Characterization of additional cell lines. A. Immunofluorescence for DAPI (blue) and CGA (red) on murine NEPC cells (TC566 TC411K). B. OPN HMC-1 cells. C. Western blot in treated or not with Brefeldin A (BFA; 5 g/ml). Vinculin was evaluated as housekeeping control. D. Gating strategy applied to distinguish human (CD49f+c-Kit-) from MCs (CD49f-c-Kit+) by flow cytometry. E. evaluation ELISA (left panel) real time PCR (the Spp1 transcript; right WT...
<p>Supplementary Figure S2. OPN evaluation in bone marrow-derived MCs. A. Separate channels of immunofluorescence for DAPI (cyan), (red) and WGA (blue) WT OPN-/- MCs showed Fig. 1A B. Representative images 3 different biological replicates (blue), These pictures were used the quantification reported 1B.</p>
<p>Supplementary Figure S4. Characterization of additional cell lines. A. Immunofluorescence for DAPI (blue) and CGA (red) on murine NEPC cells (TC566 TC411K). B. OPN HMC-1 cells. C. Western blot in treated or not with Brefeldin A (BFA; 5 g/ml). Vinculin was evaluated as housekeeping control. D. Gating strategy applied to distinguish human (CD49f+c-Kit-) from MCs (CD49f-c-Kit+) by flow cytometry. E. evaluation ELISA (left panel) real time PCR (the Spp1 transcript; right WT...