A5024051510- Cell Image Analysis Techniques
- Neuroscience and Neuropharmacology Research
- Single-cell and spatial transcriptomics
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurobiology and Insect Physiology Research
- Neural dynamics and brain function
- Neurogenesis and neuroplasticity mechanisms
- Cancer, Hypoxia, and Metabolism
- Microencapsulation and Drying Processes
- Metabolism, Diabetes, and Cancer
- Geochemistry and Geologic Mapping
- Animal Behavior and Reproduction
- Image Processing Techniques and Applications
- Agricultural and Food Sciences
- Bee Products Chemical Analysis
- Cellular transport and secretion
- Zebrafish Biomedical Research Applications
- Neural Networks and Applications
- Epigenetics and DNA Methylation
- Advanced Fluorescence Microscopy Techniques
- Congenital heart defects research
- Digital Imaging for Blood Diseases
- Biochemical and Molecular Research
- Genomic variations and chromosomal abnormalities
- Advanced Electron Microscopy Techniques and Applications
Howard Hughes Medical Institute
2019-2024
Janelia Research Campus
2019-2024
Helix (United States)
2024
McGill University
2010-2022
McGill University Health Centre
2014-2022
Montreal General Hospital
2016-2018
European Molecular Biology Laboratory
2010-2015
A good brain needs a vacuum cleaner.
Microglial cells participate in brain development and influence neuronal loss synaptic maturation. Fractalkine is an important chemokine whose expression increases during that can microglia function via the fractalkine receptor, CX3CR1. Mice lacking Cx3cr1 show a variety of defects thought to be result deficient function. Activation CX3CR1 for proper migration sites injury into development. However, little known about how modulates microglial properties Here we examined morphology, response...
Abstract Fixed human brain samples in tissue repositories hold great potential for unlocking complexities of the and its alteration with disease. However, current methodology simultaneously resolving complex three-dimensional (3D) cellular anatomy organization, as well as, intricate details cells has been limited due to weak labeling characteristics high background levels. To expose these samples, we developed a method overcome major limitations. This approach offers an unprecedented view...
The architectures of dendritic trees are crucial for the wiring and function neuronal circuits because they determine coverage receptive territories, as well nature strength sensory or synaptic inputs. Here, we describe a cell-intrinsic pathway sculpting arborization (da) neurons in Drosophila that requires Longitudinals Lacking (Lola), BTB/POZ transcription factor, its control F-actin cytoskeleton through Spire (Spir), an actin nucleation protein. Loss Lola from da reduced overall length...
Folding of newly synthesized polypeptides (NSPs) into functional proteins is a highly regulated process.Rigorous quality control ensures that NSPs attain their native fold during or shortly after completion translation.Nonetheless, signaling pathways govern the degradation in mammals remain elusive.We demonstrate stress-induced c-Jun N-terminal kinase (JNK) recruited to ribosomes by receptor for activated protein C 1 (RACK1).RACK1 an integral component 40S ribosome and adaptor...
HortaCloud is a cloud-based, open-source platform designed to facilitate the collaborative reconstruction of long-range projection neurons from whole-brain light microscopy data. By providing virtual environments directly within cloud, it eliminates need for costly and time-consuming data downloads, allowing researchers work efficiently with terabyte- scale volumetric datasets. Standardization computational resources in cloud make deployment easier more predictable. The pay-as-you-go model...
Patients with Type 6 episodic ataxia (EA6) have mutations of the excitatory amino acid transporter EAAT1 (also known as GLAST), but underlying pathophysiological mechanism for EA6 is not known. a glutamate expressed by astrocytes and other glia, it serves dual function an anion channel. One EA6-associated mutation P>R substitution (EAAT1(P>R)) that in transfected cells has reduced rate transport abnormal conductance. We this EAAT1(P>R) glial Drosophila larvae found these exhibit paralysis,...
Abstract Mice lacking serotonin receptor 1A (Htr1a) display increased anxiety behavior that depends on the expression of in forebrain during third to fifth postnatal weeks. Within forebrain, Htr1a is prominently expressed soma and dendrites CA1 pyramidal neurons hippocampus these cells undergo rapid dendritic growth synapse formation this period. Consistent with a possible role synaptic maturation, knockout mice show ramification oblique dendrites. These findings suggest may shape...
Gene expression is translationally regulated during many cellular and developmental processes. Translation can be modulated by affecting the recruitment of mRNAs to ribosome, which involves recognition 5' cap structure cap-binding protein eIF4E. Drosophila has several genes encoding eIF4E-related proteins, but biological role most them remains unknown. Here, we report that eIF4E-3 required specifically spermatogenesis. Males lacking are sterile, showing defects in meiotic chromosome...
Summary Quantification of neuronal morphology is essential for understanding connectivity and many software tools have been developed reconstruction morphometry. However, such remain domain-specific, tethered to specific imaging modalities, were not designed accommodate the rich metadata generated by recent whole-brain cellular connectomics. To address these limitations, we created SNT : a unifying framework morphometry analysis single-cell connectomics widely used Fiji ImageJ platforms. We...
<ns3:p>Background Dendritic spines are tiny protrusions found along the dendrites of neurons, and their number is a measure density synaptic connections. Altered morphology observed in several pathologies, spine formation as well morphological changes correlate with learning memory. The detection microscopy images analysis therefore prerequisite for many studies. We have developed new open-source, freely available, plugin ImageJ/FIJI, called Spot Spine, that allows measurements three...
Summary Neuronal cell types are the nodes of neural circuits that determine flow information within brain. morphology, especially shape axonal arbor, provides an essential descriptor type and reveals how individual neurons route their output across Despite importance few projection in mouse brain have been reconstructed entirety. Here we present a robust efficient platform for imaging reconstructing complete neuronal morphologies, including arbors span substantial portions We used this to...
Neuronal cell types are the nodes of neural circuits that determine flow information within brain. morphology, especially shape axonal arbor, provides an essential descriptor type and reveals how individual neurons route their output across Despite importance few projection in mouse brain have been reconstructed entirety. Here we present a robust efficient platform for imaging reconstructing complete neuronal morphologies, including arbors span substantial portions We used this to...
The food industry has been actively developing solutions to address healthy eating challenges in today's fast-paced world. One such solution is using microencapsulation techniques for bioactive compounds, particularly lipids. This study aims investigate the potential of technology enhancing stability lipids against oxidation body, which can compromise their nutritional efficacy. A bibliometric mapping was conducted Lens database, applying a combination keywords and filters identify relevant...
Effective classification of neuronal cell types requires both molecular and morphological descriptors to be collected in situ at single resolution. However, current spatial transcriptomics techniques are not compatible with imaging workflows that successfully reconstruct the morphology complete axonal projections. Here, we introduce a new methodology combines tissue clearing, submicron whole-brain two photon imaging, Expansion-Assisted Iterative Fluorescence In Situ Hybridization (EASI-FISH)...
<ns3:p>Background Dendritic spines are tiny protrusions found along the dendrites of neurons, and their number is a measure density synaptic connections. Altered morphology observed in several pathologies, spine formation as well morphological changes correlate with learning memory. The detection microscopy images analysis therefore prerequisite for many studies. We have developed new open-source, freely available, plugin ImageJ/FIJI, called Spot Spine, that allows measurements three...