A5011596408- Epigenetics and DNA Methylation
- Folate and B Vitamins Research
- RNA modifications and cancer
- Cancer-related gene regulation
- Blood Coagulation and Thrombosis Mechanisms
- Hemophilia Treatment and Research
- Hormonal Regulation and Hypertension
- Iron Metabolism and Disorders
- Blood properties and coagulation
- Trace Elements in Health
- Birth, Development, and Health
- Hemoglobinopathies and Related Disorders
- Lipoproteins and Cardiovascular Health
- Alcohol Consumption and Health Effects
- Toxin Mechanisms and Immunotoxins
- Nutrition and Health in Aging
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Drug Transport and Resistance Mechanisms
- Liver Disease Diagnosis and Treatment
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Transgenic Plants and Applications
- Metabolism and Genetic Disorders
- Genetic Syndromes and Imprinting
- Peroxisome Proliferator-Activated Receptors
- Cancer, Hypoxia, and Metabolism
University of Verona
2015-2024
Stago (France)
2023
Jean Mayer Human Nutrition Research Center on Aging
2013
Tufts University
2013
Alcohol is a well-known risk factor for hepatocellular carcinoma (HCC), but the mechanisms underlying alcohol-related hepatocarcinogenesis are still poorly understood. alters provision of methyl groups within hepatic one-carbon metabolism, possibly inducing aberrant DNA methylation. Whether specific pathways epigenetically regulated in alcohol-associated HCC is, however, unknown. The aim present study was to investigate genome-wide promoter methylation and gene expression profiles non-viral,...
Global DNA hypomethylation is an early molecular event in carcinogenesis. Whether methylation measured peripheral blood mononuclear cells (PBMCs) a clinically reliable biomarker for detection or cancer risk assessment to be established.From original sample-set of 753 male and female adults (ages 64.8 ± 7.3 years), PBMCs was 68 subjects with history at time enrollment 62 who developed during follow-up. Age- sex-matched controls prevalent incident cases (n = 58, respectively) were also...
<h3>Background</h3> Plasma factor VII concentrations (FVIIa), a marker of coronary artery disease (CAD) risk, are influenced by genetic markers at the promoter site: <i>A2</i> allele, due to <i>10bp</i> insertion position <i>−323</i>, is determinant lower FVIIa and reduced CAD while <i>−402A</i> <i>G>A</i> substitution, confers increased transcriptional activity in vitro resulting higher FVIIa. Transcriptional regulation <i>F7</i> epigenetic features is, however, still unknown as...
In addition to DNA methylation, hydroxymethylation of is recognized as a novel epigenetic mark. Primary liver cancers, i.e., hepatocellular carcinoma (HCC) and cholangiocarcinoma (CC), are highly prevalent but epigenetically poorly characterized, so far. the present study we measured global methylcytosine (mCyt) hydroxymethylcytosine (hmCyt) in HCC CC tissues peripheral blood mononuclear cell (PBMC) define mCyt hmCyt status and, accordingly, survival rate. Both were by liquid...
Abstract Background The liver hormone hepcidin regulates iron homoeostasis that is often altered in hepatocellular carcinoma ( HCC ). Epigenetic phenomena control gene expression through a dynamic fashion; therefore, considering the plasticity of both and epigenetic mechanisms their role carcinogenesis, we investigated whether HAMP ) modulated by DNA methylation, thus affecting status human . Materials methods Thirty‐two patients affected nonviral were enrolled, main clinical biochemical...
Background White blood cell count, which is inexpensive and widely available in clinical practice, has been proposed to provide prognostic information coronary artery disease (CAD). Elevated levels of white subtypes may play different roles atherothrombosis predict cardiovascular outcomes. Methods Results The association between counts mortality was evaluated 823 subjects with angiographically demonstrated clinically stable CAD an observational-longitudinal study. correlation among factor II...
Apparent mineralocorticoid excess (AME) is a rare autosomal recessive disease resulting from mutations within the hydroxysteroid (11β-dehydrogenase2 [HSD11B2]) gene causing prominent receptor activation by cortisol and hypokalemic low renin hypertension as main clinical feature. The objective of study was to characterize AME for possible novel HSD11B2 define role promoter methylation in phenotypic expression disease. Two proband brothers 10 relatives participated study. Peripheral blood...
Essentials•Activated factor (F)VII–antithrombin (FVIIa-AT) complex is an indirect marker of tissue (TF) activity.•FVIIa-AT plasma levels and TF messenger RNA (mRNA) in liver tissues were assessed for cancer.•Subjects with high mRNA cancer had increased risk mortality.•High FVIIa-AT associated both mortality.AbstractBackgroundTissue (TF), the main initiator coagulation cascade, plays a role progression prognosis. Activated VII–antithrombin considered exposure by reflecting TF-FVIIa...
Several polymorphic gene variants within one-carbon metabolism, an essential pathway for nucleotide synthesis and methylation reactions, are related to cancer risk. An aberrant DNA is a common feature in but whether the link between metabolism occurs through altered yet unclear. Aims of study were evaluate frequency hepatocellular-carcinoma, cholangiocarcinoma colon cancer, their relationship risk together with global status. Genotyping BHMT 716A>G, DHFR 19bp ins/del, MTHFD1 1958G>A, MTHFR...
Activated factor VII-anti-thrombin (FVIIa-AT) complex is a potential biomarker of pro-thrombotic diathesis reflecting FVIIa-tissue (TF) interaction and has been associated with mortality in patients coronary artery disease (CAD). Previous data indicated plasma lipids as predictors FVIIa-AT variability, lipoproteins stimulators the coagulation cascade. Our aim was to evaluate relationships between concentration broad apolipoprotein profile (including ApoA-I, ApoB, ApoC-III ApoE). Within...
Colon cancer is one of the most frequent solid tumor and simultaneous diagnosis primary colon liver metastases occurs in about fourth cases. The current knowledge on epigenetic signatures, especially those related to hydroxymethylation tissue, synchronous metastasis blood circulating cells lacking. This study aimed investigate both methylcytosine (mCyt) hydroxymethylcytosine (hmCyt) status DNA individual patients from metastases, cancer-free tissues leukocytes. Patients undergoing curative...
Polymorphisms within one-carbon metabolism genes have been largely studied in relation to cancer risk for the function of this pathway nucleotide synthesis and DNA methylation. Aims study were explore possible link among several common functional gene polymorphisms survival rate primary liver cancers, i.e., hepatocellular carcinoma cholangiocarcinoma, assess additional effect global methylation on mortality risk. Forty-seven patients genotyped ten polymorphisms: DHFR 19bp ins/del, TS...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causal agent of disease 2019 (COVID-19), in which coagulation abnormalities and endothelial dysfunction play a key pathogenic role. Tissue factor (TF) expression triggered by dysfunction. Activated VII-antithrombin (FVIIa-AT) complex reflects indirectly FVIIa-TF interaction has been proposed as potential biomarker prothrombotic diathesis. FVIIa-AT plasma concentration was measured 40 patients (30 males 10 females; 64.8 ±...
To define blood status of folate, vitamin B12, B6, homocysteine, and major one-carbon metabolism-related polymorphisms in healthy, males females donors, aged 18-65 years were evaluated. General characteristics lifestyle factors also investigated.
Background Mechanisms underlying hepatocellular carcinoma (HCC) development are largely unknown. The role of trace elements and proteins regulating metal ions homeostasis, i.e. metallothioneins (MTs), recently gained an increased interest. Object the study was to investigate promoter DNA methylation in MTs transcriptional regulation possible prognostic significance serum HCC. Methods Forty-nine HCC patients were enrolled clinically characterized. Cu, Se, Zn contents measured by Inductively...
DNA methylation is a reversible epigenetic phenomenon that can be modified by nutrients such as folate and influenced the MTHFR677C>;T polymorphism in gene‐nutrient interaction manner. In human cancer, global hypomethylation an almost universal finding. Peripheral blood mononuclear cells (PBMCs) are easily accessible humans our aim was to test PBMCs according folate‐ interaction, potential biomarker for cancer risk. Global measured of 68 subjects with history at enrollment 62 after...
Objective Apparent mineralocorticoid excess (AME) is an autosomal recessive disorder caused by the 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) enzyme deficiency, traditionally assessed measuring either urinary cortisol metabolites ratio (tetrahydrocortisol+allotetrahydrocortisol/tetrahydrocortisone, THF+5αTHF/THE) or cortisol/cortisone (F/E) ratio. Exosomal mRNA emerging diagnostic tool due to its stability in body fluids and biological regulatory function. It unknown whether exosomal...
The pathophysiology of cancer anorexia is multifactorial and unclear. Transcriptomic analysis from PBMCs RNA showed diverse patterns gene expression pathways in anorexic patients. We assessed whether the different transcriptomic signatures are modulated by DNA methylation lung patients presenting with poor appetite.
DNA hydroxymethylation has been recently recognized as a novel epigenetic mark. Primary liver cancers, hepatocellular carcinoma (HCC) and cholangiocarcinoma (CC), are highly prevalent but epigenetically poorly characterized. Aims were to determine by LC/MS/MS method methylcytosine (mCyt) hydroxymethylcytosine (hmCyt) content in HCC CC tissues compared with homologous non‐neoplastic analyze the survival rate according mCyt hmCyt levels peripheral blood mononuclear cells (PBMCs) DNA....