A5082268286- Advanced biosensing and bioanalysis techniques
- Electrochemical Analysis and Applications
- Biosensors and Analytical Detection
- Molecular Junctions and Nanostructures
- Electrocatalysts for Energy Conversion
- Electrochemical sensors and biosensors
- Epigenetics and DNA Methylation
- Hedgehog Signaling Pathway Studies
- Conducting polymers and applications
- CO2 Reduction Techniques and Catalysts
- Luminescence and Fluorescent Materials
- Organic Light-Emitting Diodes Research
- Cancer-related Molecular Pathways
- Analytical Chemistry and Sensors
- Covalent Organic Framework Applications
- Carbon dioxide utilization in catalysis
- Cancer Cells and Metastasis
- Fullerene Chemistry and Applications
- Advancements in Battery Materials
- Advanced battery technologies research
- Advanced Biosensing Techniques and Applications
- Graphene research and applications
- Synthesis and Properties of Aromatic Compounds
- Ionic liquids properties and applications
- Quantum Dots Synthesis And Properties
University of Bologna
2016-2025
Columbia University Irving Medical Center
2020-2024
Columbia University
2018-2024
Ospedale L. Bonomo
2021-2023
National Interuniversity Consortium of Materials Science and Technology
2022
ASL Roma
2021
Magna Graecia University
2021
Azienda Universitaria Ospedaliera Consorziale - Policlinico Bari
2021
Cornell University
2021
Keio University
2019
Abstract Electrochemiluminescence (ECL) is a powerful transduction technique with leading role in the biosensing field due to its high sensitivity and low background signal. Although intrinsic analytical strength of ECL depends critically on overall efficiency mechanisms generation, studies aimed at enhancing signal have mostly focused investigation materials, either luminophores or coreactants, while fundamental mechanistic are relatively scarce. Here, we discover an unexpected but highly...
Herein is reported a surface-confined microscopy based on electrochemiluminescence (ECL) that allows to image the plasma membrane of single cells at interface with an electrode. By analyzing photoluminescence (PL), ECL and AFM images mammalian CHO cells, we demonstrate that, in contrast wide-field fluorescence, emission confined immediate vicinity electrode surface only basal cell becomes luminescent. The resulting reveals details are not resolved by classic fluorescence microscopy, without...
We report here the development of coreactant-based electrogenerated chemiluminescence (ECL) as a surface-confined microscopy to image single cells and their membrane proteins. Labeling entire cell allows one demonstrate that, by contrast with fluorescence, ECL emission is only detected from fluorophores located in immediate vicinity electrode surface (i.e., 1–2 μm). Then, present potential diagnostic applications our approach, we selected carbon nanotubes (CNT)-based inkjet-printed...
Inflammation is a major risk factor for pancreatic ductal adenocarcinoma (PDAC). When occurring in the context of pancreatitis, KRAS mutations accelerate tumor development mouse models. We report that long after its complete resolution, transient inflammatory event primes epithelial cells to subsequent transformation by oncogenic KRAS. Upon recovery from acute inflammation, display an enduring adaptive response associated with sustained transcriptional and epigenetic reprogramming. Such...
Abstract The phenomenon of electrochemiluminescence (ECL) is luminescence triggered by electrochemical reactions at electrodes. Heterogeneous electron transfers are deeply dependent on electrode materials, therefore, besides the usual parameters, right choice a crucial point to address properly in order maximize emission efficiency. Many different electrodes have been studied, from metallic platinum or gold through transparent indium‐doped tin oxide carbon‐based electrodes, and others, such...
Many tumors display intracellular heterogeneity with subsets of cancer stem cells (CSC) that sustain tumor growth, recurrence, and therapy resistance. Cancer-associated fibroblasts (CAF) have been shown to support regulate CSC function. Here, we investigate the interactions between CSCs CAFs in mammary gland driven by combined activation Wnt/β-catenin Hgf/Met signaling mouse epithelial cells. In this setting, secrete Hedgehog ligand SHH, which via paracrine signaling. subsequently factors...
A novel co-reactant-free electrogenerated chemiluminescence (ECL) system is developed where Ru(bpy)32+ emission obtained on boron-doped diamond (BDD) electrodes. The method exploits the unique ability of BDD to operate at very high oxidation potential in aqueous solutions and promote conversion inert SO42– into reactive co-reactant S2O82–. This procedure rather straightforward, not requiring any particular electrode geometry, since only generated situ, interference with biological samples...
The impact of nanotechnology on analytical science is hardly overlooked. In the search for ever-increasing sensitivity in biomedical sensors, nanoparticles have been playing a unique role as, instance, ultrabright labels, and unravelling intimate mechanisms which govern their functioning mandatory design ultrasentitive devices. Herein, we investigated mechanism electrogenerated chemiluminescence (ECL) family core–shell silica–PEG (DDSNs), variously doped with Ru(bpy)32+ triethoxysilane...
Abstract Considering the depletion of fossil-fuel reserves and their negative environmental impact, new energy schemes must point towards alternative ecological processes. Efficient hydrogen evolution from water is one promising route a renewable economy sustainable development. Here we show tridimensional electrocatalytic interface, featuring hierarchical, co-axial arrangement palladium/titanium dioxide layer on functionalized multi-walled carbon nanotubes. The resulting morphology leads to...
Four Cu(I) complexes with general formulas [Cu(N^N)(2)][BF(4)] and [(P^P)Cu(N^N)][BF(4)] were prepared, where N^N stands for 2-(2-tert-butyl-2H-tetrazol-5-yl)pyridine P^P is a chelating diphosphine, namely bis-(diphenylphosphino)methane (dppm), bis-(diphenylphosphino)ethane (dppe) or bis[2-(diphenylphosphino)phenyl]ether (POP). In an acetonitrile medium, the Electro-Spray Ionization Mass Spectrometry (ESI-MS) determination provided preliminary evidence occurrence of dppm-containing complex...
Abstract The combination of highly sensitive techniques such as electrochemiluminescence (ECL) with nanotechnology sparked new analytical applications, in particular for immunoassay‐based detection systems. In this context, nanomaterials, particularly dye‐doped silica nanoparticles (DDSNPs) are high interest, since they can offer several advantages terms sensitivity and performance. work we synthesized two sets monodispersed biotinylated [Ru(bpy) 3 ] 2+ ‐doped nanoparticles, named...
Abstract Twisted two‐dimensional aromatic frameworks have been prepared by overcrowding the nodes with bulky and rigid substituents. The highly distorted framework alternating out‐of‐plane substituents results in diminished interlayer interactions that favor exfoliation dispersion of individual layers organic media.
ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTA Guide Inside Electrochemiluminescent Microscopy Mechanisms for Analytical Performance ImprovementSara RebeccaniSara RebeccaniDepartment of Chemistry "Giacomo Ciamician", University Bologna, Bologna 40127, ItalyMore by Sara RebeccaniView Biography, Alessandra ZanutAlessandra ZanutTandon School Engineering, New York University, Brooklyn, 11201, United StatesMore ZanutView Claudio Ignazio SantoClaudio SantoDepartment SantoView...
An electrogenerated chemiluminescence (ECL) system by in situ coreactant production, where Ru(bpy)32+ emission is generated at a boron-doped diamond (BDD) electrode, presented. The takes advantage of the unique properties BDD to promote oxidation carbonate (CO32-) into peroxydicarbonate (C2O62-), which further reacts with water form hydrogen peroxide (H2O2), acts as for ECL. Investigation mechanism reveals that ECL triggered reduction H2O2 hydroxyl radicals (OH•), later react reduced...
Carbon dots (CDs), defined by their size of less than 10 nm, are a class photoluminescent (PL) and electrochemiluminescent (ECL) nanomaterials that include variety carbon-based nanoparticles. However, the control properties, especially ECL, remains elusive afflicted series problems. Here, authors report CDs display ECL in water via coreactant which is dominant mechanism biosensing applications. They take advantage multicomponent bottom-up approach for preparing studying luminescence...
A versatile mechanism based on a chemical lens to control the electrochemiluminescence (ECL) spatial distribution is presented. Changing buffer capacity modifies rate of ECL reactions, and therefore thickness ECL-active layer.
Heterogeneous electrochemiluminescence (ECL) assays employing tri-n-propylamine as a co-reactant and tris(2,2′-bipyridine)ruthenium(II) ([Ru(bpy)3]2+) derivative an emissive label are integral to the majority of academic commercial applications ECL sensing. This model system is active research area constitutes basis successfully commercialized bead-based immunoassays. Herein, we propose novel approach enhancement such conventional via incorporation second metal coordination complex,...
Three-dimensional (3D) printed electrochemical devices are increasingly used in point-of-need and point-of-care testing. They show several advantages such as simple fabrication, low cost, fast response, excellent selectivity sensitivity small sample volumes. However, there only a few examples of analytical combining 3D-printed electrodes with electrochemiluminescence (ECL) detection, an detection principle widely employed clinical chemistry analysis. Herein, portable, miniaturized ECL...
Electrochemiluminescence (ECL) microscopy is an emerging technique with new applications such as imaging of single entities and cells. Herein, we have developed a bimodal bicolor approach to record both positive ECL (PECL: light-emitting object on dark background) shadow label-free (SECL: nonemissive shadowing the background luminescence) images This result simultaneous emissions [Ru(bpy)3]2+ used label cellular membrane (PECL) [Ir(sppy)3]3– dissolved in solution (SECL). By spectrally...
Enhancement of the electrochemiluminescence (ECL) tris(2,2′-bipyridine)ruthenium(II) ([Ru(bpy)3]2+) and tri-n-propylamine by redox mediators has emerged as a new approach to maximise sensitivity this mode detection. Herein, we examine influence six water-soluble iridium(III) complexes on widely used ECL system deconvoluting their respective contributions overall intensity over range applied potentials. The interconnected co-reactant these metal complexes, eliciting enhancement or quenching...
We report on the synthesis and physical chemical characterization of a class heteroleptic mononuclear cyclometalated bis(phenylpyridine)iridium(III) complexes with tetrazolate chelate ligands, such as deprotonated form 2-(1 H-tetrazol-5-yl)pyridine ( PyTzH), H-tetrazol-5-yl)pyrazine PzTzH), 5-bromo-2-(1 BrPyTzH). The electrochemical photophysical investigations resulting iridium(III) revealed rather wide span redox emission properties consequence nature ancillary ligand. In particular,...