A5063352141- Nail Diseases and Treatments
- Microbial infections and disease research
- Fungal Infections and Studies
- Plant Pathogens and Fungal Diseases
- Animal Ecology and Behavior Studies
- Toxoplasma gondii Research Studies
- Parasitic Infections and Diagnostics
- Herpesvirus Infections and Treatments
- Animal Disease Management and Epidemiology
- Plant and fungal interactions
- Infectious Diseases and Mycology
- Parasitic infections in humans and animals
- Mycotoxins in Agriculture and Food
- Molecular Biology Techniques and Applications
- Bat Biology and Ecology Studies
- Mycobacterium research and diagnosis
- Dermatological diseases and infestations
- Wildlife Ecology and Conservation
- Bacterial Identification and Susceptibility Testing
- Parasite Biology and Host Interactions
- Poxvirus research and outbreaks
- Fungal Biology and Applications
- Clostridium difficile and Clostridium perfringens research
- Antifungal resistance and susceptibility
- Parasites and Host Interactions
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche
2017-2025
Trichophyton indotineae is an emerging dermatophyte species that plays a relevant role in human healthcare. It has been associated with severe chronic skin infections and high level of terbinafine resistance. T. endemic to India, Iran, Iraq but several cases have reported Europe, recently. In this manuscript, the authors report first clinical description tinea corporis onychomycosis due indotineae. The patient was 42-year-old female from India lived Umbria (Central Italy) for last two years....
Toxoplasmosis is a re-emerging food-borne zoonosis of warm-blooded animals caused by Toxoplasma gondii, an obligate intracellular parasite with complex biological cycle. Until now, the genotypic approach for discovering putative resistance immuno-genetic markers has never been adopted T. gondii infection. Thus, this study aimed, first time, to deepen these novel insights. In particular, pigs, reared in different farms Central Italy, were phenotypically characterized serological assays...
Clostridium (C.) perfringens is the causative agent of several diseases and enteric infections in animals humans. The pathogenicity bacterium largely mediated by production a wide range toxins. Individual C. strains produce only subsets this toxin repertoire, which permits classification seven toxinotypes (A–G). In addition, variety minor toxins further characterizes single strains. aim work was to evaluate, using Polymerase Chain Reaction (PCR) assays, diversity 632 isolated Italy over 15...
European hedgehogs are an important reservoir for many pathogens of health interest. Since live in close contact with humans, potential zoonotic fungi raise significant public concerns, especially areas a high hedgehog density. From 2020 to 2023, 134 were surveyed fungi. Non-invasive methods used, such as brushing animals sterile toothbrush and taking oral rectal swabs from deceased ones (86 animals). Dermatophytes cultured on Dermasel agar identified using molecular tools, while yeasts...
Nowadays, food fortification with bioactive compounds deriving from agri-food waste is of great interest all over the world. In this work, apple pomace (AP), most abundant by-product juice manufacturing, was characterised by chemical, chromatographic and spectrophotometric analyses. AP showed valuable antioxidant activity, due to presence phenolic (8.56 mg gallic acid equivalents/g), including quercetin-3-
Tapeworms are trophically-transmitted and multi-host parasites with a complex indirect life cycle, strictly depending on predator-prey interactions. Their presence in free-living population, mainly definitive hosts, is arduous to study due the complexity of collecting fecal samples. However, epidemiological studies their frequency crucial from public health perspective, providing information food habits prey selection predators. The present aims update tapeworms detected stool samples by...
Mycoplasma infections are commonly found in the respiratory system of small ruminants; species most detected ovipneumoniae and arginini, associated with so-called "atypical non-progressive pneumonia". The pathogenic role M. pneumonia has been demonstrated sheep but still needs to be cleared goats; on other hand, arginini is not well understood, while goats seems low value. present study aims investigate aetiology died from disease using anatomopathological, histopathological molecular...
Toxoplasmosis is a zoonosis of warm-blooded animals, affecting also humans, caused by Toxo-plasma gondii, an obligate intracellular parasite with complex biological cycle. considered re-emerging and important public health threat, for the absence, up to now, efficient vaccines preventive measures. Sources transmission humans are environmental or food-borne, mainly through consumption raw undercooked meat, predominantly Sus scrofa, highly susceptible T. gondii. Mainly in last dec-ades,...
Abstract In this research communication we report on the diversity of yeast and mould species in 69 samples milk different dairy products from three plants located Umbria, central Italy. Isolates were characterised both macroscopically microscopically then identified by PCR genome sequencing ITS region D1–D2 domain large-subunit rRNA gene for filamentous fungi yeasts, respectively. Out analysed, 51 (73.9%) tested positive presence whereas moulds detected 25 (36.2%) samples. A total 9...
Nannizzia gypsea (formerly Microsporum gypseum ) belongs to geophilic dermatophytes, fungi involved in keratin degradation the soil; however, they are also found dogs and cats. Transmission humans can occur directly by contact with soil, but indirect transmission via domestic animals is reported too. The exact source of infection usually difficult assess most cases only hypothesised rarely investigated. This case report describes a kerion caused N. 2-year-old boy, where contagion was...
Tapeworm infections are among the most relevant parasitic diseases in humans and animals. Tapeworms from Genus Echinococcus particularly important as they can cause cystic or alveolar echinococcosis. A molecular screening was performed on 279 fecal samples collected carcasses of wild carnivores Central Italy using PCR targeting diagnostic fragments nad1, rrnS, nad5 genes. Samples positive for either Taenia spp. granulosus were sequenced to taxonomically identify DNA. Of samples, 134 (48.0%)...
A direct sandwich enzyme-linked immunosorbent assay (sELISA) was developed for the detection of atypical β2-toxin (CPB2) Clostridium perfringens. Polyclonal (PAbs) and monoclonal (MAbs) antibodies were previously obtained employing recombinant CPB2 produced in baculovirus system as antigen. In current study, PAbs used capture molecules, while purified MAbs conjugated to horseradish peroxidase (MAbs-HRP) toxin. 5C11E6 2G3G6 showed high reactivity, sensitivity specificity when tested on 232 C....
The reproductive cycle of an invasive alien Italian grey squirrel population was studied to understand its adaptation and limit spread, in order conserve the autochthonous red squirrel. Female male genital traits were evaluated throughout cycle, including ovary, uterus, testicle, epididymis, seminiferous tubule morphometry, germinative epithelium histology. Moreover, individual female fecundity determined by counting uterine scars. Ovary width uterus weight, length, reached their highest...
Taeniids are multi-host parasites with an indirect life cycle that strictly depends on a predator-prey relationship. Parasites complex may exhibit different degrees of host-specificity at each stage. Knowing the host breadth is fundamental concept biology and epidemiology these parasites. Morphological identification tapeworms challenging occasionally produce misdiagnosis. Thus, molecular investigations were carried out for parasitic cysts detected from muscle tissues in male roe deer...
A case of malignant catarrhal fever (MCF) occurred in a 4‑month‑old calf housed semi‑intensive herd central Italy is described. The was strict cohabitation with group domestic sheep. displayed clinical signs that resembled the acute form MCF and, after few days antibiotic and anti inflammatory therapy, died September 2016. diagnosis confirmed vivo blood by detection ovine herpesvirus type 2 DNA through real‑time PCR. At necropsy, gross post‑mortem findings were typical histological molecular...
Among the fungi responsible for deep mycosis, genus Aspergillus plays a predominant role both in human and veterinary medicine. From “One Health” perspective, infections by spp. often represent public health problem linked to specific occupational categories that could have greater risk of inhaling spores developing any respiratory disease. This preliminary investigation allowed acquire information about spread avian livestock Umbria region (Central Italy), their sensitivity antifungals,...
We have read with interest the comments and observations made regarding paper “A terbinafine sensitive Trichophyton indotineae strain in Italy: first clinical case of tinea corporis onychomycosis” [...]
In recent decades, globalization and international migration have increased the spread of infectious agents, including dermatophytes. Although considered minor infections, dermatophytoses are highly contagious, they significantly reduce quality life, inducing itching, burning, sleep disturbances, even depressive states. Moreover, increasing resistance to antifungals threats public health burdens costs for healthcare system.
Abstract Background Toxoplasmosis is one of the most common parasitic infections in both humans and animals. It a frequent cause abortion stillbirth intermediate hosts, especially sheep goats but rarely causes fatal clinical form adult Case presentation In contrast, study reports an unusual case toxoplasmosis young goat naturally infected with type II strain Toxoplasma gondii . A three-month-old female was presented dyspnea died few days later. Grossly, lungs were firm, edematous mottled...