A5075900667- Cutaneous lymphoproliferative disorders research
- Lymphoma Diagnosis and Treatment
- Autoimmune Bullous Skin Diseases
- Urticaria and Related Conditions
- Fungal Infections and Studies
- CAR-T cell therapy research
- Nail Diseases and Treatments
- Viral-associated cancers and disorders
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- T-cell and Retrovirus Studies
- CNS Lymphoma Diagnosis and Treatment
- Cancer and Skin Lesions
- Eosinophilic Disorders and Syndromes
- Cutaneous Melanoma Detection and Management
- Asthma and respiratory diseases
- Histiocytic Disorders and Treatments
- Dermatology and Skin Diseases
- Nonmelanoma Skin Cancer Studies
- Cancer-related Molecular Pathways
- Infectious Diseases and Mycology
- Plant Virus Research Studies
- Dermatological and COVID-19 studies
- Mast cells and histamine
- Bone and Joint Diseases
- Monoclonal and Polyclonal Antibodies Research
University of Milan
2015-2024
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
1999-2021
Ospedale Maggiore
1999-2021
Istituti di Ricovero e Cura a Carattere Scientifico
1996-2015
Institute of Dermatology
2009
Pyoderma gangrenosum (PG) is a rare, immune-mediated inflammatory skin disease presenting with painful ulcers having undermined edges. Less commonly, bullous and vegetative variants exist. Histology consists of neutrophil-rich dermal infiltrate. We characterized immunohistochemically the infiltrate in different PG another neutrophilic dermatosis as Sweet's syndrome. studied 21 patients PG, eight syndrome 20 controls, evaluating immunoreactivity for cell markers (CD3, CD163 myeloperoxidase),...
Although several cases of chronic urticaria (CU) are currently regarded as autoimmune in origin, associated with histamine-releasing autoantibodies, an activation blood coagulation via tissue factor (TF) and a strong expression TF lesional skin have been described. Eosinophils, which involved CU lesions, recently demonstrated the major source human blood. We assessed whether eosinophils cellular lesions.Twenty patients severe were studied. Skin biopsy specimens taken from wheals. The control...
Linear IgA bullous dermatosis (LABD) is a rare autoimmune subepithelial vesiculobullous disease due to autoantibodies directed against different antigens of the basement membrane zone (BMZ) skin and/or mucosae. It affects mainly preschool-aged children and adults, with only few studies on large series. The aim this study was assess possible differences between adults regarding clinical presentation, immunopathologic features, management course disease. A retrospective review 38 LABD...
Bullous pemphigoid (BP) is a blistering skin disease caused by autoantibodies to hemidesmosomal proteins, with eosinophils participating in blister formation. Eosinophils are source of tissue factor (TF), an initiator blood coagulation.To evaluate the local and systemic activation coagulation BP.We studied 20 patients active BP (eight re-evaluated during remission) 40 controls. The markers prothrombin fragment F1+2 d-dimer were measured plasma all subjects both fluid BP. TF was evaluated...
Primary cutaneous CD4+ small-/medium-sized pleomorphic T-cell lymphoma (CD4+ PCSM-TCL) is a rare lymphoproliferative disorder with favorable prognosis. Distinguishing it from other lymphomas often challenge.We retrospectively collected CD4+PCSM-TCL cases two centers (MD Anderson Cancer Center, USA and University of Milan, Italy) evaluated their clinicopathological features. Array-comparative genomic hybridization (aCGH) analysis was performed on 11 cases.A total 62 patients were identified....
Primary cutaneous CD8+ aggressive epidermotropic cytotoxic T-cell lymphoma (pcAECyTCL) is a rare variant of with an clinical course and very poor prognosis. Until now, neither systematic characterization genetic alterations driving pcAECyTCL has been performed, nor effective therapeutic regimes for patients have defined. Here, we present the first highresolution by using wholegenome RNA sequencing. Our study provides comprehensive description (i.e., genomic rearrangements, copy number...
The ongoing outbreak of monkeypox virus (hMPXV1) is the largest recorded in historically nonendemic countries. Genomic surveillance has emerged as a pivotal tool to track spread and monitor evolution viral pathogens. Therefore, assess genetic diversity circulating hMPXV1 northern Italy June July 2022, we sequenced analyzed five complete genomes viruses sampled from patients presenting with typical course infection. Phylogenetic analysis confirmed that all belong predominant epidemic lineage...
Primary cutaneous lymphomas are neoplasms of the immune system with a distinct tropism for skin and an absence extracutaneous manifestations at time diagnosis. Studies focusing on in children adolescents remain scarce often do not encompass rare subtypes.
<i>Background:</i> Pemphigus is a severe blistering disorder caused by autoantibodies to desmogleins 1 and 3. Because some patients with pemphigus never enter into remission, new immunosuppressants are warranted. Rituximab chimeric monoclonal antibody binding the CD20 antigen on B cells, which proved be effective in recalcitrant pemphigus. <i>Objectives:</i> To evaluate efficacy safety of rituximab refractory investigate its effects autoantibody profile....
Coagulation activation has been demonstrated in two prototypic autoimmune skin diseases, chronic urticaria and bullous pemphigoid, but only the latter is associated with increased thrombotic risk. Two markers of coagulation (prothrombin fragment F1+2 fibrin D-dimer) were measured by immunoenzymatic methods plasma samples from 30 patients active urticaria, positive for autologous serum test, pemphigoid healthy subjects. In biopsies, tissue factor expression was evaluated both...
Cutaneous metastases represent 2% of all skin tumours. Their recognition can be challenging, as they may present with different clinical features, consequent frequent delay and failure in diagnosis. To review our series cutaneous metastatic lesions, analyse their frequency according to patient gender, histotype, localization the primary tumour, site metastasis, correlate this data clinicopathological parameters. We conducted a retrospective cases from visceral neoplasms diagnosed...
Primary cutaneous acral CD8 + T‐cell lymphoma (acral TCL ) is a new provisional entity characterized by skin lesions and an indolent course. We describe extraordinary case relapsed nodules with cytotoxic infiltrates on the left ear. After 35 years, spread to other sites, mass same histological features as appeared nose. Multiple courses of chemotherapy led stable disease. Histological examinations carried out at different times showed gradual transformation neoplastic cells, increased...
Origin of basal cell carcinoma (BCC) is still unclear. We studied the cytokeratin (CK) profile in BCC using monoclonal antibodies against 12 CKs to further investigate suggested origin tumor from follicular matrix cells or outer root sheath and determine if subtypes can be identified on basis their CK profiles. Cases pilomatricoma samples fetal skin served as controls establish matrical developing follicles during intrauterine life, that epidermis cutaneous adnexa adult life having been...
Pemphigus is a group of autoimmune blistering diseases skin and/or mucous membranes caused by the presence antibodies against adhesion molecules on cell surface keratinocytes. In genetically predisposed patients, several factors, including drugs, physical agents, neoplasms, hormones, and viruses, notably herpes simplex virus (HSV), have been hypothesized to trigger or exacerbate disorder. To clarify whether HSV infection represents an aetiopathogenetic factor for pemphigus consequence...
Primary cutaneous gamma-delta T cell lymphomas (PCGDTCLs) are rare and aggressive malignancies that have been diagnostically challenging for dermopathologists clinicians since their first published descriptions in 1991. Since then, the availability of immunostaining receptors γ δ formalin-fixed paraffin-embedded samples has greatly increased our knowledge phenotype by showing it may also be present context indolent entities, such as mycosis fungoides (MFs) lymphomatoid papulosis, this raised...
Psoriasis is a common, inflammatory immune-mediated skin disease mainly presenting with plaques whose pathogenesis based on the central role of interleukin (IL)-23/IL-17 axis. However, mechanisms acting in papular lesions early-phase psoriasis are not fully understood. The aim this study was to assess involvement autoinflammation, state sterile inflammation driven by IL-1 over-production that has been recently hypothesized act early phase disease. Lesional 10 patients recent onset, untreated...
COronaVIrus Disease 19 (COVID-19) is associated with a wide spectrum of skin manifestations, but SARS-CoV-2 RNA in lesional has been demonstrated only few cases.The objective this study was to demonstrate presence samples from patients different COVID-19-related cutaneous phenotypes.Demographic and clinical data 52 COVID-19-associated manifestations were collected. Immunohistochemistry digital PCR (dPCR) performed all samples. situ hybridization (ISH) used confirm the RNA.Twenty out (38%)...
The determination of clonality has proven to be a useful adjunct the diagnosis cutaneous lymphocytic infiltrates. It is considered particularly helpful for distinction mycosis fungoides (MF) and inflammatory dermatoses.To verify sensitivity polymerase chain reaction (PCR)-heteroduplex analysis T-cell receptor gamma-chain gene (TCRgamma) rearrangements in patients with MF establish whether clinicopathological re-evaluation lesions previously unclassified or non-neoplastic entities but found...