A5061248345- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Genomics and Phylogenetic Studies
- Inflammatory Bowel Disease
- Metabolomics and Mass Spectrometry Studies
- RNA and protein synthesis mechanisms
- Gut microbiota and health
- Ocular Surface and Contact Lens
- Glycosylation and Glycoproteins Research
- Machine Learning in Bioinformatics
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Pancreatic function and diabetes
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Musculoskeletal pain and rehabilitation
- Advanced Biosensing Techniques and Applications
- Antimicrobial Peptides and Activities
- Salivary Gland Disorders and Functions
- Bacteriophages and microbial interactions
- Cancer, Hypoxia, and Metabolism
- Pain Mechanisms and Treatments
- Prostate Cancer Treatment and Research
- Peptidase Inhibition and Analysis
- Bacterial Genetics and Biotechnology
UNSW Sydney
2016-2025
The University of Sydney
2013-2025
Bioanalytica (Switzerland)
2013-2023
University College Dublin
2008
Royal North Shore Hospital
2007
University of Technology Sydney
2007
Garvan Institute of Medical Research
1999-2004
St Vincent's Hospital Sydney
2000-2003
Australian Genome Research Facility
1997
Abstract A protein map of the smallest known self‐replicating organism, Mycoplasma genitalium (Class: Mollicutes), revealed a high proportion acidic proteins. Amino acid composition was used to putatively identify, or provide unique parameters, for 50 gene products separated by two‐dimensional gel electrophoresis. further 19 proteins were subjected peptide‐mass fingerprinting using matrix‐assisted laser desorption ionisation‐time flight (MALDI‐TOF) mass spectrometry and 4 N ‐terminal Edman...
Abstract HUPO initiated the Plasma Proteome Project (PPP) in 2002. Its pilot phase has (1) evaluated advantages and limitations of many depletion, fractionation, MS technology platforms; (2) compared PPP reference specimens human serum EDTA, heparin, citrate‐anti‐coagulated plasma; (3) created a publicly‐available knowledge base (www.bioinformatics.med.umich.edu/hupo/ppp; www.ebi.ac.uk/pride). Thirty‐five participating laboratories 13 countries submitted datasets. Working groups addressed...
Insulin stimulates the translocation of glucose transporter GLUT4 from intracellular vesicles to plasma membrane. In present study we have conducted a comprehensive proteomic analysis affinity-purified 3T3-L1 adipocytes discover potential regulators trafficking. addition previously identified components storage including insulin-regulated aminopeptidase and vesicle soluble N-ethylmaleimide factor attachment protein (v-SNARE) VAMP2, three new Rab proteins, Rab10, Rab11, Rab14, on vesicles. We...
The accurate quantitation of proteins and peptides in complex biological systems is one the most challenging areas proteomics. Mass spectrometry-based approaches have forged significant in-roads allowing sensitive ability to multiplex vastly samples through application robust bioinformatic tools. These relative absolute quantitative measures using label-free, tags, or stable isotope labelling their own strengths limitations. continuous development these methods vital for increasing...
Introduction Breast cancer is a complex heterogeneous disease and leading cause of death in women. Early diagnosis monitoring progression breast are important for improving prognosis. The aim this study was to identify protein biomarkers urine early screening detection invasive progression. Method We performed comparative proteomic analysis using ion count relative quantification label free LC-MS/MS from patients (n = 20) healthy control women 20). Results Unbiased LC-MS/MS-based proteomics...
Triple-negative breast cancer (TNBC) is the most aggressive subtype. Radiotherapy an effective option for treatment of TNBC; however, acquired radioresistance a major challenge to modality. In this study, we show that integrated stress response (ISR) activated signaling pathway in radioresistant TNBC cells. The constitutive phosphorylation eIF2α cells promotes activation ATF4 and elicits transcription genes implicated glutathione biosynthesis, including GCLC, SLC7A11, CTH, which increases...
Prostate cancer (PCa) is a global health concern, ranking as the most common among men in Western countries. Traditional diagnostic methods are invasive with adverse effects on patients. Due to heterogeneous nature of PCa and their multifocality, tissue biopsies often yield false-negative results. To address these challenges, researchers exploring innovative approaches, particularly realms proteomics metabolomics, identify more reliable biomarkers improve diagnosis. Liquid biopsy (LB) has...
Identifying biomarkers and signaling pathways are important for the management of prostate cancer (CaP) radioresistance. In this study, we identified differential proteins from parental CaP cell lines radioresistant (RR) sublines using a label-free LC-MS/MS proteomics approach. A total 309 pathway were to be significantly altered between CaP-RR cells (p ≤ 0.05, fold differences >1.5, ≥80% power). Among these proteins, nineteen common among three paired associated with metastasis, progression...
Analysis of complex protein samples by two-dimensional electrophoresis (2-DE) is often more difficult in the presence a few predominant proteins. In plasma, proteins such as albumin mask lower abundance, well significantly limiting amount that can be loaded onto immobilized pH gradient strip. this paper Gradiflow, preparative system, has been used to deplete human plasma highly abundant under native and denatured conditions. A three step protocol incorporating charge separation collect with...
Mycoplasma genitalium is the smallest member of class Mollicutes, with a genome size 580 kb. It has potential to express 480 gene products, and therefore considered be an excellent model assess: (a) minimum metabolism required by free living cell; (b) proteomic technologies information obtained proteome analysis. Here, we report on most complete observed at 73% (expected proteome), analysed 33% (reported proteome). The use four overlapping pH windows in conjunction SDS/PAGE allowed 427...
Abstract The data collected by Human Proteome Organization's Plasma Pilot project phase was analyzed members of our working group. Accordingly, a functional annotation the human plasma proteome carried out. Here, we report findings analyses. First, bioinformatic analyses were undertaken to determine likely sources proteins and develop protein interaction network identified in this project. Second, these performed context subproteomes involved coagulation pathway, mononuclear phagocytic...
The protein kinase Akt mediates several metabolic and mitogenic effects of insulin, whereas activation C (PKC) isoforms has been implicated in the inhibition insulin action. We have previously shown that both PKC PKCepsilon are activated skeletal muscle insulin-resistant high fat-fed rats, to identify potential substrates for these kinases, we incubated recombinant with rat fractions vitro. specifically phosphorylated a 48-kDa was subsequently identified by mass spectrometry as Ndrg2. Ndrg2...
The glutathione shunt is one of the most important contributors to cellular redox state, with implications across cancer, chronic diseases, diseases ageing, and autoimmune including inflammatory bowel disease (IBD). Traditionally, state gauged by ratio surrogate metabolites GSH GSSG. However, this presents methodological challenges offers a constrained illustration without systems-level understanding dynamics, failing elucidate variations an entire biochemical network. Targeted proteomics...
Abstract This is the first 2‐DE study using sequential dyes to analyse phospho‐, glyco‐ and total tear protein profiles (Pro‐Q Diamond for phosphoprotein, Pro‐Q Emerald glycoprotein Sypro Ruby protein). method minimised gel–gel variations, allowing better comparisons among three generated a whole map of PTM protein. A novel protein, dermcidin, was identified time in this study. The identification antimicrobial suggests new model defence tears. In addition, we are able present experimental...