A5101826735- Advanced Proteomics Techniques and Applications
- Glycosylation and Glycoproteins Research
- Cellular Mechanics and Interactions
- Mass Spectrometry Techniques and Applications
- Biocrusts and Microbial Ecology
- Slime Mold and Myxomycetes Research
- Microtubule and mitosis dynamics
- Molecular Biology Techniques and Applications
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Metabolomics and Mass Spectrometry Studies
- Genomics and Phylogenetic Studies
- Protist diversity and phylogeny
- Carbohydrate Chemistry and Synthesis
- Microfluidic and Bio-sensing Technologies
- Cellular transport and secretion
- Galectins and Cancer Biology
- Microfluidic and Capillary Electrophoresis Applications
- Bacterial Genetics and Biotechnology
- Fish Ecology and Management Studies
- Lipid Membrane Structure and Behavior
- Machine Learning in Bioinformatics
- Viral Infectious Diseases and Gene Expression in Insects
- Nuclear Structure and Function
- Ubiquitin and proteasome pathways
Macquarie University
1993-2014
University of Florida
2012
Cardiff University
2010
Johnson Space Center
2009
Australian Genome Research Facility
1997-2003
University of Verona
2003
Yale University
2003
National Institute for Medical Research
2003
Proteos (United States)
2000
Proteome Sciences (United Kingdom)
2000
Abstract A protein map of the smallest known self‐replicating organism, Mycoplasma genitalium (Class: Mollicutes), revealed a high proportion acidic proteins. Amino acid composition was used to putatively identify, or provide unique parameters, for 50 gene products separated by two‐dimensional gel electrophoresis. further 19 proteins were subjected peptide‐mass fingerprinting using matrix‐assisted laser desorption ionisation‐time flight (MALDI‐TOF) mass spectrometry and 4 N ‐terminal Edman...
Abstract Bacteriophage P1 Cre/loxP based systems can be used to manipulate the genomes of mice in vivo and vitro, allowing generation tissue‐specific conditional mutants. Wehave generated mouse lines expressing Cre recombinase hematopoietic tissues using vav regulatory elements, or lymphoid cells hCD2 promoter locus control region (LCR). The R26R‐EYFP reporter line was determine pattern expression each enabled assessment activity at a single‐cell level. Analysis showed that elements were...
Outer membrane proteins (OMPs) of Gram-negative bacteria are key molecules that interface the cell with environment. Traditional biochemical and genetic approaches have yielded a wealth knowledge relating to function OMPs. Nonetheless, completion Escherichia coli genome sequencing project there is opportunity further expand our understanding organization, expression OMPs in this bacterium. In report we describe proteomic approach which provides platform for parallel analysis We propose rapid...
Abstract In this study, dithiothreitol was replaced by tributyl phosphine as the reducing agent in both sample solution for first‐dimensional isoelectric focusing and during immobilised pH gradient (IPG) equilibration procedure. Tributyl improves protein solubility focusing, which results shorter run times increased resolution. is nonionic thus does not migrate IPG, therefore maintaining conditions course of separation. The provided maintenance gives improved decreased horizontal streaking...
Abundant and hydrophilic nonmembrane proteins with isoelectric points below pH 8 are the predominant identified in most proteomics projects. In yeast, however, low-abundance make up 80% of predicted proteome, approximately 50% have pI's above 30% yeast ORFs to encode membrane at least 1 trans-membrane span. By applying highly solubilizing reagents fractionation a fraction we purified 780 protein isoforms, representing 323 gene products, including 28% low abundance 49% or associated proteins....
Abstract Amino acid analysis and matrix‐assisted laser desorption/ionisation time‐of‐flight (MALDI‐TOF) mass spectrometry were used to identify nine of twelve proteins originally separated by two‐dimensional electrophoresis derived from an organism poorly defined at the molecular level ( Spiroplasma melliferum ). Two three unidentified appeared be novel. The percentage amino composition peptide fragments generated tryptic digestion search PIR/SWISS‐PROT MOWSE databases respectively. Lists...
The Dictyostelium discoideum cell surface antigen PsA is a glycoprotein which first appears in the multicellular stage soon after tip formation and selectively expressed on prespore cells. D19 gene encodes an mRNA sequence highly enriched over prestalk cells slug stage. We have determined 81 amino acid residues of N-terminal from immunoaffinity-purified protein shown this to be identical predicted gene. There are several short repeat elements close C terminus, unequal crossing-over within...
Abstract. We describe Global Atmosphere 6.0 and Land 6.0: the latest science configurations of Met Office Unified Model JULES land surface model developed for use across all timescales. includes ENDGame dynamical core, which significantly increases mid-latitude variability improving a known bias. Alongside developments model’s physical parametrisations, also in tropics, leads to an improved representation tropical cyclones other phenomena. Further atmospheric parametrisations improve aspects...
Abstract To understand the changes in protein expression associated with various physiological states as well development of pathological eye disease, we have begun to map components normal human reflex tears. An analytical reference tears was created using two‐dimensional polyacrylamide gel electrophoresis (2‐D PAGE) pH 3.5–10 immobilized gradients (IPGs). Micropreparatively loaded gels were transferred polyvinylidene difluoride (PVDF) and analysed by a combination N ‐terminal sequence...
Carbamylation is widely quoted as being a problem in 2-D gel analysis and the associated sample preparation steps. This modification occurs when iso-cyanate, urea break-down product, covalently modifies lysine residues, thus inducing change isoelectric point. Urea used at up to 9 M concentrations gels because of its ability disrupt protein structure effect denaturation without need for ionic surfactants such SDS. We have studied carbamylation using 7 2 thiourea, under range experimental...
The human red blood cell sialoglycoprotein, glycophorin A (GpA), contains a 'mucin-like' extensively O-glycosylated extracellular domain which carries the MN group antigens. We have revised sites of O-glyccsylation in GpA by automated solid-phase Edman degradation, allowed positive identification and quantitation Ser Thr residues, as well single N-glycosylation site. One N-linked 16 O-linked were identified. Carbohydrate was absent on 1, Ser14, Ser15, Ser23, Thr28 Thr58 GpA. propose that...
Abstract The development of automated, high throughput technologies for the rapid identification proteins is essential large‐scale proteome projects. While a degree automation already exists in some stages protein process, such as automated acquisition matrix assisted laser desorption ionisation — time flight (MALDI‐TOF) mass spectra, efficient interfaces between different are still lacking. We report highly integrated system large scale separated by two‐dimensional gel electrophoresis...
Thiabendazole and cambendazole induced mitotic arrest isogenic diploid formation through chromosome doubling in amoebae of the cellular slime mould Dictyostelium discoideum grown either axenically or a bacterial suspension. The effect nocodazole on cells was similar to that thiabendazole cambendazole, but with bacteria much less effective inducer did not lead formation. Benomyl, active ingredient ben late, had little no induction poor diploids bacteria. All four benzimidazole derivatives...