A5059644822- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Thyroid Cancer Diagnosis and Treatment
- Botany and Geology in Latin America and Caribbean
- Bacterial Identification and Susceptibility Testing
- Growth Hormone and Insulin-like Growth Factors
- Colorectal Cancer Treatments and Studies
- Lung Cancer Treatments and Mutations
- Analytical chemistry methods development
- Nitric Oxide and Endothelin Effects
- Machine Learning in Bioinformatics
- Food Allergy and Anaphylaxis Research
- Ion-surface interactions and analysis
- Allergic Rhinitis and Sensitization
- Biochemical Analysis and Sensing Techniques
- Neuroscience and Neuropharmacology Research
- Contact Dermatitis and Allergies
- Ocular Surface and Contact Lens
- Chemical Reactions and Isotopes
- Glycosylation and Glycoproteins Research
- Redox biology and oxidative stress
- Pituitary Gland Disorders and Treatments
- Ubiquitin and proteasome pathways
Johns Hopkins University
2001-2024
Target (United States)
2019-2020
University of Colorado Health
2000-2019
University of Colorado Anschutz Medical Campus
2001-2019
UNSW Sydney
1993-2019
Helix Biomedix (United States)
2009-2019
Weed Science Society of America
2019
University of Colorado Denver
2009-2018
King Saud University
2009-2015
University of California, Los Angeles
2014
Abstract A protein map of the smallest known self‐replicating organism, Mycoplasma genitalium (Class: Mollicutes), revealed a high proportion acidic proteins. Amino acid composition was used to putatively identify, or provide unique parameters, for 50 gene products separated by two‐dimensional gel electrophoresis. further 19 proteins were subjected peptide‐mass fingerprinting using matrix‐assisted laser desorption ionisation‐time flight (MALDI‐TOF) mass spectrometry and 4 N ‐terminal Edman...
Peroxynitrite, a powerful mutagenic oxidant and nitrating species, is formed by the near diffusion-limited reaction of ·NO O 2⨪ during activation phagocytes. Chronic inflammation induced phagocytes major contributor to cancer other degenerative diseases. We examined how γ-tocopherol (γT), principal form vitamin E in United States diet, α-tocopherol (αT), supplements, protect against peroxynitrite-induced lipid oxidation. Lipid hydroperoxide formation liposomes (but not isolated low-density...
Some but not all patients with non-small-cell lung cancer (NSCLC) respond to treatment epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs). We developed and tested the ability of a predictive algorithm based on matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) analysis pretreatment serum identify who are likely benefit from EGFR TKIs.Serum collected NSCLC before gefitinib or erlotinib were analyzed by MALDI MS. Spectra acquired independently at...
Hypochlorous acid is the major strong oxidant generated by human neutrophils, and it has potential to cause much of tissue damage that these inflammatory cells promote. It produced from hydrogen peroxide chloride heme enzyme myeloperoxidase. To unequivocally establish hypochlorous contributes inflammation, a stable unique marker for its reaction with biomolecules needs be identified. In this investigation we have found reagent reacts tyrosyl residues in small peptides converts them...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTProtein-bound 3,4-dihydroxyphenylalanine is a major reductant formed during hydroxyl radical damage to proteinsSteven P. Gieseg, Jeremy A. Simpson, Tim S. Charlton, Mark W. Duncan, and Roger T. DeanCite this: Biochemistry 1993, 32, 18, 4780–4786Publication Date (Print):May 11, 1993Publication History Published online1 May 2002Published inissue 11 1993https://pubs.acs.org/doi/10.1021/bi00069a012https://doi.org/10.1021/bi00069a012research-articleACS...
We conducted an investigation of the levels neurotoxin 2-amino-3-(methylamino)-propanoic acid (BMAA) in cycad flour. Analysis 30 flour samples processed from endosperm Cycas circinalis seeds collected on Guam indicated that more than 87% total BMAA content was removed during processing. Furthermore, ½ almost all (≥99%) removed. found no significant regional differences prepared several villages Guam. Testing different by same Chamorro woman over 2 years suggests washing procedure probably...
Bacterial lipopolysaccharide (LPS) evokes several functional responses in the neutrophil that contribute to innate immunity. Although certain responses, such as adhesion and synthesis of tumor necrosis factor-α, are inhibited by pretreatment with an inhibitor p38 mitogen-activated protein kinase, others, actin assembly, unaffected. The aim present study was investigate changes gene transcription expression following exposure establish their dependence on signaling. Microarray analysis...
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS) is used to obtain fast and accurate determinations of molecular mass, but quantitative are generally made by other techniques. In this study we illustrate the practical utility automated MALDI-TOFMS as a tool for quantifying diverse array biomolecules covering an extensive weight range, present in biological extracts fluids. Growth hormone was measured rat pituitary tissue; insulin human pancreatic...
Knowledge of the peptide and protein components seminal fluid their role in prostate diseases including benign prostatic hyperplasia carcinoma is scant. We have undertaken a proteomic analysis semen as forerunner to identifying sensitive specific diagnostic markers diseases; aid improved therapeutic intervention; and, enhance our understanding health disease.Peptide pooled human (n = 5) were separated by gel electrophoresis (1D 2D) identified either matrix-assisted laser desorption...
In order to better understand basic mechanisms of tumor development and identify potential new biomarkers, we have performed difference gel electrophoresis (DIGE) peptide mass fingerprinting on pooled protein extracts from patients with papillary thyroid carcinoma (PTC) compared matched normal tissue. Image analysis DIGE gels comparing PTC tissue indicated that 25% the spots were differentially expressed at a 2.5-fold cutoff 35% two-fold. Comparison between two different pools tissues...
Abstract Amino acid analysis and matrix‐assisted laser desorption/ionisation time‐of‐flight (MALDI‐TOF) mass spectrometry were used to identify nine of twelve proteins originally separated by two‐dimensional electrophoresis derived from an organism poorly defined at the molecular level ( Spiroplasma melliferum ). Two three unidentified appeared be novel. The percentage amino composition peptide fragments generated tryptic digestion search PIR/SWISS‐PROT MOWSE databases respectively. Lists...
Abstract Internal standards were used to demonstrate that matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry can be applied the quantitative analysis of low molecular weight polar compounds. Three examples tested: a standard curve for 3,4‐dihydroxyphenylalanine (DOPA) was prepared using stable isotope analogue (i.e., [ 13 C 6 ]DOPA) as an internal standard; 2 H 16 ]‐acetylcholine employed quantification acetylcholine; and in final example, peptide Ac‐Ser‐Ile‐Arg‐His‐Tyr‐NH...
Summary Rationale An important property of allergens is their ability to cross‐link IgE and activate mast cells basophils. The effector activity peanut has not been well characterized. Methods Crude extracts fresh flour were fractionated by gel filtration. Effector function was assayed measuring degranulation RBL SX‐38 sensitized with from individual sera pools peanut‐allergic donors. Results Following filtration, 75 ± 7% the applied protein 76 16% ( n =3) (assayed a pool 11 sera) recovered...
To unlock the full potential of disease modifying treatments, it is essential to develop early biomarkers for Alzheimer's (AD). For practical reasons, blood-based markers that could provide a signal at stage mild cognitive impairment (MCI) or even earlier would be ideal. Using proteomic approach isobaric tagging relative and absolute quantitation (iTRAQ), we compared plasma protein profiles MCI, AD, cognitively normal control subjects from two independent cohorts: Sydney Memory Ageing Study...
We have determined whether oral estrogen reduces the biological effects of growth hormone (GH) in GH-deficient (GHD) women compared with transdermal treatment. In two separate studies, eight GHD randomly received either or for 8 wk before crossing over to alternate route administration. The first study assessed incremental doses GH (0.5, 1.0, 2.0 IU/day 1 each) on insulin-like factor I (IGF-I) levels during each treatment phase. second (2 IU/day) lipid oxidation and protein metabolism using...
We compared the value of plasma samples with that 24-hour urine in identifying patients pheochromocytoma among those hypertension. employed specific gas chromatographic–mass spectrometric analysis both and for simultaneous assay norepinephrine its neuronal metabolite 3,4-dihydroxyphenylglycol (DHPG). The study population consisted 1086 hypertension, them 25 proved pheochromocytoma. Reference ranges free DHPG were established. Measurement provided best index a This technique had 100 percent...
Proteomics is the large-scale analysis of protein profiles. This approach has not yet been reported in study acute lung injury (ALI). details profiles plasma and pulmonary edema fluid (EF) from 16 ALI patients bronchoalveolar lavage (BALF) 12 normal subjects. More than 300 distinct spots were evident EF BALF both subjects patients. Of these, 158 identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. In profile patients, there multiple qualitative changes....
2-Amino-3-(methylamino)-propanoic acid (BMAA) is a neurotoxic, excitatory amino which has been linked through cycad use and consumption with the onset of variant amyotrophic lateral sclerosis occurring high incidence in western Pacific region. We have studied BMAA pharmacokinetics, oral bioavailability blood-brain barrier permeability rat an attempt to better define possible role for this disease. To evaluate its kinetics uptake, (25-400 mg/kg) was administered rats, either acutely or...
Abstract: β‐ N ‐Methylamino‐ l ‐alanine (BMAA) is a neuro‐toxic plant amino acid that has been implicated in the pathogenesis of high incidence amyotrophic lateral sclerosis and related parkinsonism dementia western Pacific. Previous studies have demonstrated BMAA taken up into brain following intravenous or oral administration. To examine kinetics mechanism transfer, influx across blood–brain barrier was measured rats using an situ perfusion technique. found to be saturable with maximal...