A5045670551- Ocular Surface and Contact Lens
- Vascular Malformations Diagnosis and Treatment
- Malaria Research and Control
- Reproductive Biology and Fertility
- Mosquito-borne diseases and control
- Corneal Surgery and Treatments
- Intracranial Aneurysms: Treatment and Complications
- Advanced Glycation End Products research
- Drug-Induced Adverse Reactions
- Insect Resistance and Genetics
- Vector-borne infectious diseases
- Food Allergy and Anaphylaxis Research
- Salivary Gland Disorders and Functions
- Advancements in Transdermal Drug Delivery
- Intracerebral and Subarachnoid Hemorrhage Research
- Viral Infectious Diseases and Gene Expression in Insects
- Glaucoma and retinal disorders
- Renal and related cancers
- Connexins and lens biology
- Ocular Infections and Treatments
- Electrocatalysts for Energy Conversion
- Mitochondrial Function and Pathology
- Contact Dermatitis and Allergies
- Mast cells and histamine
- Spondyloarthritis Studies and Treatments
Yantai University
2011-2024
Peking University
2024
Sichuan University
2011-2024
West China Second University Hospital of Sichuan University
2024
North University of China
2023
Shanghai Jiao Tong University
2022-2023
Liaoning Academy of Agricultural Sciences
2007-2022
Renji Hospital
2019-2022
Macquarie University
2013-2020
Sinopec (China)
2019
Liposomes have been well established as an effective drug delivery system, due to simplicity of their preparation and unique characteristics. However conventional liposomes are unsuitable for the on-demand content release, which limits therapeutic utility. Here we report X-ray-triggerable incorporating gold nanoparticles photosensitizer verteporfin. The 6 MeV X-ray radiation induces verteporfin produce singlet oxygen, destabilises liposomal membrane causes release cargos from cavity. This...
In this study, the ability of carboxymethylcellulose (CMC), used in artificial tear formulations, to interact with corneal-epithelial-cells (HCECs) and facilitate corneal epithelial wound healing was investigated.HCECs were incubated fluorescein-labeled CMC (F-CMC). CMC-epithelial binding measured by spectrophotometry. The effect on F-CMC hyaluronic acid (HA) or glucose after preincubation HA, mAb CD44, glucose, GluT-1. fibronectin collagen incubating proteins F-CMC. widths 18 hours...
Purpose. To quantitatively detect proteins and cholesterol extracted from worn silicone hydrogel contact lenses determine the effect of various lens care solutions on deposit accumulation. Methods. Contact lenses, made different polymers a daily wear schedule with solutions, were collected. Lipid protein deposits by methanol:chloroform (1:1, v/v) extraction solution (containing urea surfactant), respectively. extracts separated quantified using thin layer chromatography. Protein standard...
To examine the deposition of tear phospholipids and cholesterol onto worn contact lenses effect lens material care solution.Lipids were extracted from tears using 2:1 chloroform:methanol extract washed with aqueous ammonium acetate, before analysis by electrospray ionization tandem mass spectrometry (ESI-MS/MS).Twenty-three molecular lipids sphingomyelin (SM) phosphatidylcholine (PC) classes detected in tears, total concentrations each class determined to be 5 +/- 1 pmol/microL (...
Nested PCR is considered a sensitive and specific method for detecting malaria parasites especially useful in epidemiological surveys. However, the preparation of DNA templates often time-consuming costly. A simplified was developed to directly use small blood filter paper square (2 × 2 mm) as template after treatment with saponin. This paper-based nested (FP-PCR) compared microscopy standard extracted by using Qiagen mini kit from spots 204 febrile cases. The FP-PCR technique further...
Purpose. To correlate clinical responses during contact lens wear with the amount of protein or cholesterol extracted from lenses after wear. Methods. Clinical parameters, including adverse response rates and corneal staining, symptomatology rating were collected a series tests comprising four different silicone hydrogel multipurpose solutions. test for correlates, total daily compared statistically to parameters. Results. The (p = 0.008) 0.01) was higher those subjects who showed...
Purpose. The aim of the study is to determine adhesion Gram positive and negative bacteria onto conventional hydrogel (CH) silicone (SH) contact lens materials with without lysozyme, lactoferrin, albumin coating. Methods. Four types (three SH—balafilcon A, lotrafilcon B, senofilcon A; one CH—etafilcon A) were coated or (uncoated lenses acted as controls) then incubated in Staphylococcus aureus (Saur 31) either two strains Pseudomonas aeruginosa (Paer 6294 6206) for 24 h at 37°C. total counts...
As a secreted fluid, the state of tear glycosylation is particularly important in role immunity ocular surface. Tears are valuable source non-invasive biomarkers for disease and there continued efforts to characterize their components thoroughly. In this study, small volume basal tears (5 μL) was collected from healthy controls, patients with diabetes without retinopathy retinopathy. The detailed N- O-linked protein glycome characterized relative abundance each structure determined. Of 50...
Cellular senescence is associated with aging and considered a potential contributor to age-associated neurodegenerative disease. Exposure ionizing radiation increases the risk of developing premature neurovascular degeneration dementia but also induces senescence. As cells cerebrovascular endothelium are particularly susceptible play an important role in brain homeostasis, we investigated radiation-induced microvascular endothelial (EC). Using biotinylation label surface proteins,...
The Greater Mekong Subregion is aiming to achieve regional malaria elimination by 2030. Though a shift in parasite species predominance Plasmodium vivax has been recently documented, the transmission of two minor species, malariae and ovale spp., poorly characterized region. This study aims determine prevalence these China–Myanmar border area their genetic diversity. Epidemiology was conducted during passive case detection hospitals clinics Myanmar four counties China along border....
Abstract This is the first 2‐DE study using sequential dyes to analyse phospho‐, glyco‐ and total tear protein profiles (Pro‐Q Diamond for phosphoprotein, Pro‐Q Emerald glycoprotein Sypro Ruby protein). method minimised gel–gel variations, allowing better comparisons among three generated a whole map of PTM protein. A novel protein, dermcidin, was identified time in this study. The identification antimicrobial suggests new model defence tears. In addition, we are able present experimental...
Rapid diagnostic tests (RDTs) have become an essential tool in the contemporary malaria control and management programmes world. This study aims to evaluate performance of two commonly used RDTs for diagnosis China-Myanmar border area.A total 606 febrile patients were recruited this diagnosed infections by microscopy, (Pf/Pan device, Pv/Pf device) nested PCR.Malaria parasites found 143 which 51, 73, 19 Plasmodium falciparum, vivax P. falciparum/P. mixed infections, respectively. Compared...
Studies indicate that contact lens (CL) discontinuation mostly occurs because of dryness and discomfort symptoms. This study aimed to investigate relationships between changes in the concentration tear inflammatory mediators with subjective comfort ratings CL wear no morning evening.Forty-five subjects collected tears twice daily evening or without lenses. Comfort was rated subjectively on a scale from 1 100 (where extremely comfortable) just before each collection. Tear samples were assayed...
Understanding naturally acquired immunity to infections caused by Plasmodia in different malaria endemicity settings is needed for better vaccine designs and exploring antibody responses as a proxy marker of transmission intensity. This study investigated the sero-epidemiology along international border between China Myanmar, where elimination action plans are place. recruited 233 P. vivax 156 falciparum infected subjects with acute at clinics hospitals. In addition, 93 67 healthy...
Bisphenol A (BPA), an endocrine disruptor, is a widely used chemical that has adverse effects on animal development and reproduction. The current research aimed to evaluate the effect of BPA in vitro maturation (IVM) subsequent embryo mouse oocytes following fertilization (IVF). IVM was performed presence different concentrations (0, 20, 50, or 100 μg/mL) BPA. Nuclear maturation, IVF efficiency embryonic were determined. levels reactive oxygen species (ROS) glutathione (GSH) (50 group...
Transmission-blocking vaccine (TBV) is a promising strategy to interfere with the transmission of malaria. To date, only limited TBV candidate antigens have been identified for Plasmodium vivax. HAP2 gamete membrane fusion protein, homology class II viral proteins. Herein we reported characterization PvHAP2 its potential as P. The HAP2/GCS1 domain was expressed in baculovirus expression system and recombinant protein used raise antibodies rabbits. Indirect immunofluorescence assays showed...
Abstract The Mediterranean mussel, Mytilus galloprovincialis , is a significant marine bivalve species that has ecological and economic importance. This robustly resilient highly invasive. Despite the scientific commercial interest in studying its biology aquaculture, there remains need for high-quality, chromosome-scale reference genome. In this study, we have assembled high-quality genome M. . total length of our 1.41 Gb, with scaffold N50 sequence 96.9 Mb. BUSCO analysis revealed 97.5%...
Rabbits have a longer inter-blink time (approximately 10 minutes) compared with humans (five to eight seconds), suggesting that rabbits much more stable tear film. Using fluorescein, the break-up of has been reported be similar humans. This study set out measure in using non-invasive methods and establish pattern humans.The was measured observed six New Zealand White on two separate occasions both Keeler Tearscope-plus(TM) slitlamp biomicroscope.The mean rabbit 29.8 ± 3.4 (SD) minutes....