A5050883534- Anaerobic Digestion and Biogas Production
- Gut microbiota and health
- Microbial metabolism and enzyme function
- Cardiac Ischemia and Reperfusion
- Monoclonal and Polyclonal Antibodies Research
- Acute Myeloid Leukemia Research
- Cardiac Arrest and Resuscitation
- Vasculitis and related conditions
- Anesthesia and Neurotoxicity Research
- Plant Reproductive Biology
- Acute Lymphoblastic Leukemia research
- Plant Molecular Biology Research
- Antimicrobial Peptides and Activities
- Advanced Proteomics Techniques and Applications
- Biochemical and Structural Characterization
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antioxidant Activity and Oxidative Stress
- Sarcoidosis and Beryllium Toxicity Research
- Legume Nitrogen Fixing Symbiosis
- Erythropoietin and Anemia Treatment
- Cardiac and Coronary Surgery Techniques
- Cannabis and Cannabinoid Research
- Microbial Metabolic Engineering and Bioproduction
- Probiotics and Fermented Foods
- Biofuel production and bioconversion
The University of Melbourne
2000-2021
AgriBio
2015
Austin Hospital
1995
The global demand for affordable carbon has never been stronger, and there is an imperative in many industrial processes to use waste streams make products. Gas-fermenting acetogens offer a potential solution several commercial gas fermentation plants are currently under construction. As energy limits acetogen metabolism, supply of H2 should diminish substrate loss CO2 facilitate production reduced energy-intensive However, the effects on CO-grown have yet be experimentally quantified...
Mutations in the interleukin-7 receptor (IL7R) or Janus kinase 3 (JAK3) occur frequently T-cell acute lymphoblastic leukemia (T-ALL) and both are able to drive cellular transformation development of T-ALL mouse models. However, signal transduction pathways downstream JAK3 mutations remain poorly characterized. Here we describe phosphoproteome JAK3(L857Q)/(M511I) activating transformed Ba/F3 lymphocyte cells. Signaling regulated by mutants were assessed following inhibition JAK1/JAK3 using...
Small, post-translationally modified and secreted peptides regulate diverse plant developmental processes. Due to low natural abundance, it is difficult isolate identify these peptides. Using an improved peptide isolation protocol Orbitrap mass spectrometry, nine 15-amino-acid CEP were identified that corresponded the two domains encoded by Medicago truncatula CEP1 (MtCEP1). Novel arabinosylated hydroxylated in root cultures overexpressing MtCEP1. The five most abundant species detected also...
Remote Ischemic Preconditioning (RIPC) induced by brief episodes of ischemia the limb protects against multi-organ damage ischemia-reperfusion (IR). Although it has been demonstrated that RIPC affects gene expression, proteomic response to not determined. This study aimed examine changes in plasma proteome. Five healthy adult volunteers had 4 cycles 5 min alternating with reperfusion forearm. Blood samples were taken from ipsilateral arm prior first ischaemia, immediately after each episode...
Multigene families encoding diverse secreted peptide hormones play important roles in plant development. A need exists to efficiently elucidate the structures and post-translational-modifications of these difficult-to-isolate planta so that their biological functions can be determined. mass spectrometry bioinformatics approach was developed comprehensively analyze peptidome Medicago hairy root cultures xylem sap. We identified 759 spectra corresponding products twelve including four CEP...
Saliva is easily obtainable from a large number of animals in noninvasive manner and contains wide diversity compounds including hormones, metabolites, proteins that may be good source biomarkers health disease. Here we have used combination multidimensional prefractionation, targeted, glycocapture methodologies to profile the bovine salivary proteome. The nontargeted approach four different separation consisting SDS-PAGE, Off-gel fractionation, RP-HPLC, SCX-HPLC. In targeted approach, we've...
Anti-proteinase 3 (PR3) and anti-myeloperoxidase (MPO) autoantibodies are present in many patients with Wegener's granulomatosis (WG) microscopic polyarteritis. The aim of this study was to determine whether these antibodies bound linear peptide sequences on their target antigens. If common epitopes were demonstrated, then could be manufactured used diagnostic ELISAs for anti-PR3 anti-MPO antibodies. In addition, any homology between bacterial or viral might implicate those microorganisms...
Background Remote ischemic preconditioning (RIPC) has been applied in paediatric cardiac surgery. We have demonstrated that RIPC induces a proteomic response plasma of healthy volunteers. tested the hypothesis modifies children undergoing Tetralogy Fallot (TOF) repair. Methods and Results Children (n=40) were randomized to control groups. Blood was sampled at baseline, after cardiopulmonary bypass (CPB) 6, 12 24h post-CPB. Plasma analysed by liquid chromatography mass spectrometry (LC-MS) an...
The revised legislation on medicinal cannabis has triggered a surge of research studies in this space. Yet, proteomics is lagging. In previous study, we optimised the protein extraction mature buds for bottom-up proteomics. follow-up developed top-down mass spectrometry (MS) strategy to identify intact denatured from apical buds. After testing different source-induced dissociation (SID), collision-induced (CID), higher-energy collisional (HCD), and electron transfer (ETD) parameters infused...
C-TERMINALLY ENCODED PEPTIDEs (CEPs) control diverse responses in plants including root development, system architecture, nitrogen demand signalling, and nutrient allocation that influences yield, there is evidence different ligands impart phenotypic responses. Thus, a need for simple method identifies bona fide CEP hormone-receptor pairings vivo examines whether family peptides bind the same receptor. We used formaldehyde or photoactivation to cross-link fluorescently tagged group 1 2 CEPs...
Cardiopulmonary bypass (CPB) can be associated with deleterious clinical effects. However, the impact of CPB on inflammatory, immunological and other homeostatic pathways remains poorly understood. We investigated plasma proteome in children undergoing tetralogy Fallot repair.Blood samples were taken from 20 prior to at end 6h, 12h 24h after CPB. Plasma was analysed by liquid chromatography-mass spectrometry (LC-MS) a label-free, untargeted approach. Data using Genedata software identify...