A5017127721- Bacterial Genetics and Biotechnology
- Biochemical and Structural Characterization
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Vibrio bacteria research studies
- Amyotrophic Lateral Sclerosis Research
- Plant Disease Resistance and Genetics
- Cancer, Stress, Anesthesia, and Immune Response
- Veterinary medicine and infectious diseases
- Alzheimer's disease research and treatments
- Proteoglycans and glycosaminoglycans research
- Antimicrobial Peptides and Activities
- DNA Repair Mechanisms
- Microbial infections and disease research
- Neuropeptides and Animal Physiology
- Bacterial biofilms and quorum sensing
- Toxin Mechanisms and Immunotoxins
- DNA and Nucleic Acid Chemistry
- Neuroendocrine Tumor Research Advances
- Cholinesterase and Neurodegenerative Diseases
- Antimicrobial Resistance in Staphylococcus
- Machine Learning in Bioinformatics
- Plant Reproductive Biology
The University of Sydney
2008-2025
New South Wales Department of Primary Industries
2009
University of Wollongong
2009
Macquarie University
2006
Australian Genome Research Facility
2006
Campylobacter jejuni is a gastrointestinal pathogen that able to modify membrane and periplasmic proteins by the N-linked addition of 7-residue glycan at strict attachment motif (D/E)XNX(S/T). Strategies for comprehensive analysis targets glycosylation, however, are hampered resistance glycan-peptide bond enzymatic digestion or β-elimination have previously concentrated on soluble glycoproteins compatible with lectin affinity gel-based approaches. We developed strategies enriching C. HB93-13...
Multigene families encoding diverse secreted peptide hormones play important roles in plant development. A need exists to efficiently elucidate the structures and post-translational-modifications of these difficult-to-isolate planta so that their biological functions can be determined. mass spectrometry bioinformatics approach was developed comprehensively analyze peptidome Medicago hairy root cultures xylem sap. We identified 759 spectra corresponding products twelve including four CEP...
Abstract Frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS) are neurodegenerative diseases that considered to be on the same disease spectrum because of overlapping genetic, pathological clinical traits. Changes in serum proteins FTD ALS poorly understood, currently no definitive biomarkers exist for diagnosing or monitoring progression either disease. Here we applied quantitative discovery proteomics analyze protein changes (N = 72) 28) patient compared controls 22)....
Abstract Campylobacter jejuni is the leading cause of food‐ and water‐borne illness world‐wide. The membrane‐associated proteome a recent C. gastrointestinal isolate (JHH1) was generated by sodium carbonate precipitation ultracentrifugation followed 2‐DE MALDI‐TOF MS as well 2‐DLC (strong cation exchange RP chromatography) trypsin digests coupled to MS/MS (2‐DLC/MS/MS). 2‐DE/MS identified 77 proteins, 44 which were predicted membrane while 2‐DLC/MS/MS 432 206 be associated. A total 453...
Mycoplasma hyopneumoniae induces respiratory disease in swine by colonizing cilia causing ciliostasis, cilial loss and epithelial cell death. Heparin binds to M. cells a dose-dependent manner blocks its ability adhere porcine cilia. We show here that Mhp493 (P216), paralogue of the cilium adhesin P97 (Mhp183), is cleaved between amino acids 1040 1089 generating surface-accessible, heparin-binding proteins P120 P85. Antiphosphoserine antibodies recognized P85 2-D immunoblotting studies TiO(2)...
Pancreatic cancer (PC) is a highly metastatic malignancy. More than 80% of patients with PC present advanced-stage disease, preventing potentially curative surgery. The neuropeptide Y (NPY) system, best known for its role in controlling energy homeostasis, has also been shown to promote tumorigenesis range types, but yet be explored. We show that expression NPY and NPY1R are up-regulated mouse models human PC. Moreover, using the genetically engineered, autochthonous KP R172H C model PC, we...
Campylobacter jejuni is the most common cause of bacterial gastroenteritis in developed world. Immunoproteomics highlighted a 42-45 kDa antigen that comigrated on two-dimensional (2-DE) gels with C. major outer membrane protein (MOMP). Predictive analysis revealed two candidates for identity antigen, likely which was surface-associated lipoprotein, JlpA. Recombinant JlpA (rJlpA) reacted patient sera, confirming antigenic. Polyclonal antibodies raised against rJlpA 3 mass variants from...
Abstract Pseudomonas aeruginosa is a ubiquitous pathogen most typically associated with wound infections, but also the main cause of mortality in patients suffering from cystic fibrosis (CF). The ability to adapt oxidative stress host immune defense may be one mechanism by which P. establishes infection lung and eventually out‐competes other pathogenic bacteria persist into chronic infection. We utilized proteomics approach identify proteins response PAO1 hydrogen peroxide...
Inositol tris/tetrakis phosphate kinases (IP3-4K) in the human fungal priority pathogens, Cryptococcus neoformans (CnArg1) and Candida albicans (CaIpk2), convey numerous virulence functions, yet it is not known whether IP3-4K catalytic activity or a scaffolding role responsible. We therefore generated C. strain with non-functional kinase, referred to as dead-kinase (dk) CnArg1 (dkArg1). verified that, although dkARG1 cDNA cloned from this produced protein expected molecular weight, dkArg1...
The segregation of prokaryotic plasmids typically requires a centromere-like site and two proteins, centromere-binding protein (CBP) an NTPase. By contrast, single 245 residue Par mediates partition the prototypical staphylococcal multiresistance plasmid pSK1 in absence identifiable NTPase component. To gain insight into centromere binding by its function we performed structural, biochemical vivo studies. Here show that binds consisting seven repeat elements. We demonstrate this...
The repair of double-strand DNA breaks (DSBs) by homologous recombination is crucial in the maintenance genome integrity. While key role Mre11-Rad50-Nbs1 (MRN) complex well known, hSSB1 (SOSSB and OBFC2B), one main components sensor single-stranded (SOSS) protein complex, has also been shown to rapidly localize DSB promote repair. We have previously demonstrated that binds directly Nbs1, a component MRN damage-independent manner. However, recruitment an interaction between Integrator Complex...
Plant transmembrane proteins (TMPs) are essential for normal cellular homeostasis, nutrient exchange, and responses to environmental cues. Commonly used bottom–up proteomic approaches fail identify a broad coverage of peptide fragments derived from TMPs. Here, we mass spectrometry (MS) compare the effectiveness two solubilization protein cleavage methods shoot-derived TMPs legume Medicago. We compared urea solubilization, trypsin Lys-C (UR-TLC) method formic acid cyanogen bromide (FA-CTLC)...
The segregation of prokaryotic plasmids typically requires a centromere-like site and two proteins, centromere-binding protein (CBP) an NTPase. By contrast, single 245 residue Par mediates partition the prototypical staphylococcal multiresistance plasmid pSK1 in absence NTPase component. To gain insight into centromere binding by its function we performed structural, biochemical vivo studies. Here show that binds consisting seven repeat elements. We demonstrate this Par-centromere...