Brooke A. Pereira
A5085027792- Pancreatic and Hepatic Oncology Research
- Cancer Cells and Metastasis
- Neuroendocrine Tumor Research Advances
- Epigenetics and DNA Methylation
- Pancreatic function and diabetes
- Ferroptosis and cancer prognosis
- Caveolin-1 and cellular processes
- Cellular Mechanics and Interactions
- Amino Acid Enzymes and Metabolism
- 3D Printing in Biomedical Research
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- Phagocytosis and Immune Regulation
- Prostate Cancer Treatment and Research
- Cancer Genomics and Diagnostics
- Cell Adhesion Molecules Research
- Proteoglycans and glycosaminoglycans research
- Biomedical Ethics and Regulation
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Mast cells and histamine
- Biomedical and Engineering Education
- Microbial metabolism and enzyme function
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Immune Cell Function and Interaction
UNSW Sydney
2019-2025
Garvan Institute of Medical Research
2019-2025
The Kinghorn Cancer Centre
2019-2025
St Vincent's Clinic
2019-2023
St Vincent's Health
2023
Cancer Australia
2021
Monash University
2015-2020
Discovery Institute
2019
Abstract Heterogeneous subtypes of cancer-associated fibroblasts (CAFs) coexist within pancreatic cancer tissues and can both promote restrain disease progression. Here, we interrogate how cells harboring distinct alterations in p53 manipulate CAFs. We reveal the existence a p53-driven hierarchy, where with gain-of-function (GOF) mutant educate dominant population CAFs that establish pro-metastatic environment for GOF null alike. also demonstrate educated by may be reprogrammed either or...
The tumour stroma, and in particular the extracellular matrix (ECM), is a salient feature of solid tumours that plays crucial role shaping their progression. Many desmoplastic including breast cancer involve significant accumulation type I collagen. However, recently it has become clear precise distribution organisation molecules such as collagen equally important abundance. Cancer-associated fibroblasts (CAFs) coexist within tissues play both pro- anti-tumourigenic roles through remodelling...
Abstract The lysyl oxidase family represents a promising target in stromal targeting of solid tumors due to the importance this crosslinking and stabilizing fibrillar collagens its known role tumor desmoplasia. Using small-molecule drug-design approaches, we generated validated PXS-5505, first-in-class highly selective potent pan-lysyl inhibitor. We demonstrate vitro vivo that inhibition decreases chemotherapy-induced pancreatic desmoplasia stiffness, reduces cancer cell invasion metastasis,...
Abstract Cancer-associated fibroblasts (CAF) are major contributors to pancreatic ductal adenocarcinoma (PDAC) progression through protumor signaling and the generation of fibrosis, latter which creates a physical barrier drugs. CAF inhibition is thus an ideal component any therapeutic approach for PDAC. SLC7A11 cystine transporter that has been identified as potential target in PDAC cells. However, no prior study evaluated role tumor stroma its prognostic significance. Here we show high...
In prostate cancer, cancer-associated fibroblasts (CAF) exhibit contrasting biological properties to non-malignant (NPF) and promote tumorigenesis. Resolving intercellular signaling pathways between CAF tumor epithelium may offer novel opportunities for research translation. To this end, the proteome phosphoproteome of four pairs patient-matched NPF were characterized identify discriminating proteomic signatures. Samples analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
Abstract The poor prognosis of pancreatic ductal adenocarcinoma (PDAC) is attributed to the highly fibrotic stroma and complex multi-cellular microenvironment that difficult fully recapitulate in pre-clinical models. To fast-track translation therapies inform personalised medicine, we aimed develop a whole-tissue ex vivo explant model maintains viability, 3D multicellular architecture, microenvironmental cues human tumours. Patient-derived surgically-resected PDAC tissue was cut into 1–2 mm...
Here we report the DNA methylation profile of 84 sporadic pancreatic neuroendocrine tumors (PanNETs) with associated clinical and genomic information. We identified three subgroups PanNETs, termed T1, T2 T3, distinct patterns methylation. The T1 subgroup was enriched for functional ATRX, DAXX MEN1 wild-type genotypes. contained mutations in recurrent chromosomal losses half genome no association between regions loss levels. were larger had lower MGMT gene body, which showed positive...
Intravital imaging guides a personalized medicine approach to target mechanoreciprocity in pancreatic cancer.
Pancreatic ductal adenocarcinoma (PDAC) is characterized by increasing fibrosis, which can enhance tumor progression and spread. Here, we undertook an unbiased temporal assessment of the matrisome highly metastatic KPC (
Pancreatic cancer (PC) is a highly metastatic malignancy. More than 80% of patients with PC present advanced-stage disease, preventing potentially curative surgery. The neuropeptide Y (NPY) system, best known for its role in controlling energy homeostasis, has also been shown to promote tumorigenesis range types, but yet be explored. We show that expression NPY and NPY1R are up-regulated mouse models human PC. Moreover, using the genetically engineered, autochthonous KP R172H C model PC, we...
Prostate cancer changes the phenotype of cells within stromal microenvironment, including fibroblasts, which in turn promote tumour progression. Functional prostate cancer-associated fibroblasts (CAFs) coincide with alterations DNA methylation levels at loci-specific regulatory regions. Yet, it is not clear how these compare across CAFs from different patients. Therefore, we examined consistency and prognostic significance genome-wide profiles between patients grades primary cancer.We used...
The dense desmoplastic and fibrotic stroma is a characteristic feature of pancreatic ductal adenocarcinoma (PDAC), regulating disease progression, metastasis response to treatment. Reciprocal interactions between the tumour are mediated by bidirectional integrin-mediated signalling, in particular Focal Adhesion Kinase (FAK). FAK often hyperactivated overexpressed aggressive cancers, promoting stromal remodelling inducing tissue stiffness which can accelerate cancer cell proliferation,...
Mast cells (MCs) are important cellular components of the tumor microenvironment and significantly associated with poor patient outcomes in prostate cancer other solid cancers. The promotion progression partly involves heterotypic interactions between MCs cancer-associated fibroblasts (CAFs), which combine to potentiate a pro-tumor extracellular matrix promote epithelial cell invasion migration. Thus far, CAFs remain poorly understood. To identify molecular changes that may alter resident MC...
Aberrant AKT activation occurs in a number of cancers, metabolic syndrome, and immune disorders, making it an important target for the treatment many diseases. To monitor spatial temporal activity live setting, we generated Akt-FRET biosensor mouse that allows longitudinal assessment using intravital imaging conjunction with image stabilization optical window technology. We demonstrate sensitivity various cancer models verify its suitability to response drug targeting spheroid organotypic...
ABSTRACT Most of the techniques used for micro and nano‐patterning currently are high quality reproducibility, but they require expensive equipment involve many time‐consuming steps to achieve desired results. We herein report a patterning method conducting layers on breathable substrates using fast, simple, mask‐less laser engraving technique. A resolution in range 30 μm has been successfully achieved this report. The is fast pattern can be easily changed or redesigned within few minutes....