Gail P. Risbridger
A5089881929- Prostate Cancer Treatment and Research
- TGF-β signaling in diseases
- Sperm and Testicular Function
- Hormonal and reproductive studies
- Estrogen and related hormone effects
- Epigenetics and DNA Methylation
- Prostate Cancer Diagnosis and Treatment
- Cancer, Lipids, and Metabolism
- Testicular diseases and treatments
- Cancer Cells and Metastasis
- Kruppel-like factors research
- Sexual Differentiation and Disorders
- Urinary Bladder and Prostate Research
- Immunotherapy and Immune Responses
- Heat shock proteins research
- Metabolism, Diabetes, and Cancer
- Phytoestrogen effects and research
- Urological Disorders and Treatments
- Ubiquitin and proteasome pathways
- Reproductive Biology and Fertility
- Cancer Mechanisms and Therapy
- Urologic and reproductive health conditions
- Molecular Biology Techniques and Applications
- Cancer Research and Treatments
- Peptidase Inhibition and Analysis
Monash University
2016-2025
The University of Melbourne
2006-2025
Peter MacCallum Cancer Centre
2013-2025
Australian Regenerative Medicine Institute
2005-2024
Discovery Institute
2018-2024
Cabrini Hospital
2022-2024
John Wiley & Sons (United States)
2015-2021
Johns Hopkins University
2019-2021
Johns Hopkins Medicine
2019-2021
Sidney Kimmel Comprehensive Cancer Center
2019-2021
In recent years the Illumina HumanMethylation450 (HM450) BeadChip has provided a user-friendly platform to profile DNA methylation in human samples. However, HM450 lacked coverage of distal regulatory elements. have now released MethylationEPIC (EPIC) BeadChip, with new content specifically designed target these regions. We used and whole-genome bisulphite sequencing (WGBS) perform critical evaluation EPIC array platform. covers over 850,000 CpG sites, including >90 % CpGs from an additional...
Prostate cancer depends on fatty acid uptake and remodeling of the prostate lipidome, blocking impairs tumorigenesis.
Prostate cancers (PCs) with loss of the potent tumor suppressors TP53 and RB1 exhibit poor outcomes. also influence cell plasticity are frequently lost in PCs neuroendocrine (NE) differentiation. Therapeutic strategies that address these aggressive variant urgently needed. Using deep genomic profiling 410 metastatic biopsies, we determine relationships between combined PC phenotypes. Notably, 40% TP53/RB1-deficient tumors classified as AR-active adenocarcinomas, indicating NE differentiation...
Abstract Germline mutations in the BRCA2 tumour suppressor are associated with both an increased lifetime risk of developing prostate cancer (PCa) and aggressive disease. To understand this aggression, here we profile genomes methylomes localized PCa from 14 carriers deleterious germline ( -mutant PCa). We show that harbour genomic instability a mutational more closely resembles metastastic than shows epigenomic dysregulation MED12L / MED12 axis, which is frequently dysregulated metastatic...
It was recently demonstrated that antiestrogens prevented prostate cancer (PRCA) in men. The source of estradiol (E2) contributes to carcinogenesis, as well the selected estrogen receptor (ER) signaling pathway, is unknown. To evaluate estrogen's effects we developed a new model PRCA utilizing testosterone and E2 stimulate PRCA. determine whether local situ production affected incidence PRCA, aromatase-knockout (ArKO) mice were evaluated. In contrast wild-type mice, ArKO had reduced...
Prostate cancer (PCa) and benign prostatic hyperplasia (BPH) are androgen-dependent diseases commonly treated by inhibiting androgen action. However, ablation or castration fail to target androgen-independent cells implicated in disease etiology recurrence. Mechanistically different castration, this study shows beneficial proapoptotic actions of estrogen receptor–β (ERβ) BPH PCa. ERβ agonist induces apoptosis stromal, luminal castrate-resistant basal epithelial estrogen-deficient aromatase...
Tissue-specific aromatase production is significant in breast cancer and osteoporosis. Prostatic expression has been equivocal, any local actions of estrogens are considered secondary to centrally mediated androgen suppression. We examine estrogen biosynthesis the human prostate. Pure samples stroma epithelia from biopsy tissues were isolated by laser capture microdissection. Aromatase protein was detected Western blot analysis, mRNA RT-PCR, enzyme activity tritiated water assay, whereas...
Abstract Background: Epigenetic alterations are common in prostate cancer, yet how these modifications contribute to carcinogenesis is poorly understood. We investigated whether specific histone prognostic for cancer relapse, and the expression of epigenetic genes altered tumorigenesis. Methods: Global levels H3 lysine-18 acetylation (H3K18Ac) lysine-4 dimethylation (H3K4diMe) were assessed immunohistochemically a cohort 279 cases. gene was silico by analysis microarray data from 23 primary...
The growth and progression of solid tumors involves dynamic cross-talk between cancer epithelium the surrounding microenvironment. To date, molecular profiling has largely been restricted to epithelial component tumors; therefore, features underpinning persistent protumorigenic phenotype tumor microenvironment are unknown. Using whole-genome bisulfite sequencing, we show for first time that cancer-associated fibroblasts (CAFs) from localized prostate display remarkably distinct enduring...
In prostate cancer, cancer-associated fibroblasts (CAF) exhibit contrasting biological properties to non-malignant (NPF) and promote tumorigenesis. Resolving intercellular signaling pathways between CAF tumor epithelium may offer novel opportunities for research translation. To this end, the proteome phosphoproteome of four pairs patient-matched NPF were characterized identify discriminating proteomic signatures. Samples analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
Although androgens are the main steroids controlling growth of mammalian prostate, increasing evidence demonstrates that estrogens also regulate prostate development and growth. This study describes effects estrogen deficiency using aromatase knockout mice (ArKO) with targeted disruption cyp19 gene. Serum tissue testosterone 5α-dihydrotestosterone as well serum PRL levels significantly (P < 0.05) elevated in mature male ArKO mice. Histological, stereological, immunohistochemical studies...