A5061031098- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Anaerobic Digestion and Biogas Production
- Microbial metabolism and enzyme function
- Genomics and Phylogenetic Studies
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
- Advanced Proteomics Techniques and Applications
- Gut microbiota and health
- Algal biology and biofuel production
- Microbial Fuel Cells and Bioremediation
- CO2 Reduction Techniques and Catalysts
- Protein purification and stability
- Bacteriophages and microbial interactions
- Protein Structure and Dynamics
- Bacterial Genetics and Biotechnology
- Bioinformatics and Genomic Networks
- Gene Regulatory Network Analysis
- Microbial Community Ecology and Physiology
- Fungal and yeast genetics research
- Supercapacitor Materials and Fabrication
- Electrochemical sensors and biosensors
- Enzyme Structure and Function
- Subcritical and Supercritical Water Processes
- biodegradable polymer synthesis and properties
University of Tartu
2019-2024
The University of Queensland
2017-2022
Tallinn University of Technology
2009-2014
Competence Centre on Health Technologies (Estonia)
2012-2013
Regulation levels of the gene expression cascade controlling protein and metabolic fluxes for cells to achieve faster growth have not been elaborated in acceptable detail. Furthermore, there is need specific rate (μ) dependent absolute quantitative transcriptome proteome data understand molecular relationships enabling modify μ. We address these questions, first time, by presenting regulatory strategies more efficient metabolism Escherichia coli at higher μ statistical covariance analysis...
Cells usually respond to changing growth conditions with a change in the specific rate (μ) and adjustment of their proteome adapt maintain metabolic efficiency. Description principles behind resource allocation is important for understanding regulation response μ. Thus, we analysed dynamics Escherichia coli into different processes E. was grown on minimal defined rich media steady state continuous cultures at μ characterised combining two LC-MS/MS-based proteomics methods: stable isotope...
The global demand for affordable carbon has never been stronger, and there is an imperative in many industrial processes to use waste streams make products. Gas-fermenting acetogens offer a potential solution several commercial gas fermentation plants are currently under construction. As energy limits acetogen metabolism, supply of H2 should diminish substrate loss CO2 facilitate production reduced energy-intensive However, the effects on CO-grown have yet be experimentally quantified...
BRIEF RESEARCH REPORT article Front. Bioeng. Biotechnol., 27 March 2020 | https://doi.org/10.3389/fbioe.2020.00204
Abstract Utilising one-carbon substrates such as carbon dioxide, methane, and methanol is vital to address the current climate crisis. Methylotrophic metabolism enables growth energy generation from methanol, providing an alternative sugar fermentation. Saccharomyces cerevisiae important industrial microorganism for which on would be relevant. However, its ability metabolize has been poorly characterised. Here, using adaptive laboratory evolution 13 C-tracer analysis, we discover that S. a...
Microbes able to convert gaseous one-carbon (C1) waste feedstocks are increasingly important transition the sustainable production of renewable chemicals and fuels. Acetogens interesting biocatalysts since gas fermentation using Clostridium autoethanogenum has been commercialised. However, most acetogen strains need complex nutrients, display slow growth, not robust for bioreactor fermentations. In this work, we used three different independent adaptive laboratory evolution (ALE) strategies...
Living biological systems display a fascinating ability to self-organize their metabolism. This ultimately determines the metabolic robustness that is fundamental controlling cellular behavior. However, fluctuations in metabolism can affect homeostasis through transient oscillations. For example, yeast cultures exhibit rhythmic oscillatory behavior high cell-density continuous cultures. Oscillatory provides unique opportunity for quantitating of metabolism, as cells respond changes by...
Abstract Background Growth substrates, aerobic/anaerobic conditions, specific growth rate (μ) etc. strongly influence Escherichia coli cell physiology in terms of size, biomass composition, gene and protein expression. To understand the regulation behind these different phenotype properties, it is useful to know carbon flux patterns metabolic network which are generally calculated by analysis (MFA). However, rarely composition determined balance carefully measured same experiments could...
Microbial electrosynthesis enables the production of value-added chemicals from CO2 and electrons provided by an electrode. Clostridium ljungdahlii is electroactive acetogen that potentially could be used in microbial systems. However, optimal operational parameters for using C. are not known. Here, we explored effects yeast extract, pH, cathode potential. A low initial pH increased rate acetate H2 serum bottle cultures. When cultivated bioelectrochemical systems, coulombic efficiency (i.e....
Microbes that can recycle one-carbon (C1) greenhouse gases into fuels and chemicals are vital for the biosustainability of future industries. Acetogens most efficient known microbes fixing carbon oxides CO2 CO. Understanding proteome allocation is important metabolic engineering as it dictates fitness. Here, we use absolute proteomics to quantify intracellular concentrations >1,000 proteins in model acetogen Clostridium autoethanogenum grown autotrophically on three gas mixtures (CO, CO+H2,...
Gas fermentation offers both fossil carbon-free sustainable production of fuels and chemicals recycling gaseous solid waste using gas-fermenting microbes. Bioprocess development, systems-level analysis biocatalyst metabolism, engineering cell factories are advancing the widespread deployment commercialised technology. Acetogens particularly attractive biocatalysts but effects key physiological parameter-specific growth rate (μ)-on acetogen metabolism gas bioprocess have not been established...
Fossil resources must be replaced by renewable in production systems to mitigate green-house gas emissions and combat climate change. Electro-fermentation utilizes a bioelectrochemical system (BES) valorize industrial municipal waste. Current electro-fermentation research is mainly focused on microbial electrosynthesis using CO 2 for producing commodity chemicals replacing petroleum-based infrastructures. However, slow rates low titers of metabolites during -based impede its implementation...
OPINION article Front. Bioeng. Biotechnol., 06 February 2015Sec. Systems Biology Archive Volume 3 - 2015 | https://doi.org/10.3389/fbioe.2015.00011
Nutrient-limited continuous cultures in chemostats have been used to study microbial cell physiology for over 60 years. Genome instability and genetic heterogeneity are possible uncontrolled factors cultivation experiments. We investigated these issues by using high-throughput (HT) DNA sequencing characterize samples from different phases of a glucose-limited accelerostat (A-stat) experiment with Escherichia coli K-12 MG1655 duration regularly studies (20 generations cultivation). Seven...