Lorena Azevedo de Lima

ORCID: 0000-0001-5735-035X
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About
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Research Areas
  • Microbial Metabolic Engineering and Bioproduction
  • Biofuel production and bioconversion
  • Genomics and Phylogenetic Studies
  • Protein Hydrolysis and Bioactive Peptides
  • RNA and protein synthesis mechanisms
  • Phytase and its Applications
  • Bacteriophages and microbial interactions
  • Anaerobic Digestion and Biogas Production
  • Enzyme Production and Characterization
  • Algal biology and biofuel production
  • Fungal and yeast genetics research
  • Traditional and Medicinal Uses of Annonaceae
  • Medicinal Plant Research
  • Genetic diversity and population structure
  • Agricultural and Food Sciences
  • Plant Gene Expression Analysis
  • Rural Development and Agriculture
  • Diverse Scientific Research Studies
  • Identification and Quantification in Food
  • Rural and Ethnic Education
  • Pomegranate: compositions and health benefits
  • Nanoparticles: synthesis and applications
  • Medicinal Plants and Neuroprotection
  • Enzyme Catalysis and Immobilization
  • Stroke Rehabilitation and Recovery

University of Tartu
2022-2024

Universidade Federal do Rio Grande do Norte
2011-2024

Unichristus
2021

Universidade Federal de Viçosa
2018-2020

AgroBio
2018

Agrico (Netherlands)
2018

Universidade do Estado do Amazonas
2014-2015

Microbes able to convert gaseous one-carbon (C1) waste feedstocks are increasingly important transition the sustainable production of renewable chemicals and fuels. Acetogens interesting biocatalysts since gas fermentation using Clostridium autoethanogenum has been commercialised. However, most acetogen strains need complex nutrients, display slow growth, not robust for bioreactor fermentations. In this work, we used three different independent adaptive laboratory evolution (ALE) strategies...

10.1016/j.nbt.2024.06.002 article EN cc-by New Biotechnology 2024-06-12

Gas fermentation offers both fossil carbon-free sustainable production of fuels and chemicals recycling gaseous solid waste using gas-fermenting microbes. Bioprocess development, systems-level analysis biocatalyst metabolism, engineering cell factories are advancing the widespread deployment commercialised technology. Acetogens particularly attractive biocatalysts but effects key physiological parameter-specific growth rate (μ)-on acetogen metabolism gas bioprocess have not been established...

10.3389/fbioe.2022.879578 article EN cc-by Frontiers in Bioengineering and Biotechnology 2022-04-12

ABSTRACT Microbes able to convert gaseous one-carbon (C1) waste feedstocks are increasingly important transition the sustainable production of renewable chemicals and fuels. Acetogens interesting biocatalysts since gas fermentation using Clostridium autoethanogenum has already been commercialised. However, most acetogen strains need complex nutrients, display slow growth, not robust for routine bioreactor fermentations. In this work, we used three different independent adaptive laboratory...

10.1101/2023.01.28.526018 preprint EN bioRxiv (Cold Spring Harbor Laboratory) 2023-01-29

Aims Yeasts produce 2-phenylethanol (2-PE) from sugars via de novo synthesis; however, its synthesis is limited due to feedback inhibition on the isofunctional 3-deoxy-d-arabino-heptulosonate-7-phosphate (DAHP) synthases (Aro3p and Aro4p). This work aimed select Kluyveromyces marxianus mutant strains with improved capacity 2-PE sugars. Methods Results CCT 7735 were selected UV irradiation coupled screening of p-fluoro-dl-phenylalanine (PFP) tolerant culture medium without l-Phe addition....

10.1111/jam.14793 article EN Journal of Applied Microbiology 2020-07-24

As proteases colagenolíticas são enzimas capazes de hidrolisar as ligações peptídicas vários tipos colágeno e têm grande importância na medicina em aplicações terapêuticas. O objetivo desta pesquisa foi avaliar a produção por Bacillus stearothermophilus. Os tratamentos foram realizados meio um planejamento fatorial completo 2³, fim significância dos efeitos interações das variáveis - pH inicial, concentração substrato temperatura sobre protease colagenolítica. ponto central executado...

10.1590/1809-4392201305074 article PT cc-by-nc Acta Amazonica 2014-07-21

Qualitative analyses were carried out on solid medium with insoluble collagen 0.25% (w/v) to detect proteases collagenolytic activity produced by Bacillus sp. In cultures incubated for 24 h, a 23 full factorial design four repetitions at the center point was developed analyze effects and interactions between initial pH, temperature concentration of gelatin. Based results first design, successive performed. The most favorable production conditions found be 1.5% gelatin, pH 9.0 37 °C enzymatic...

10.1590/s1517-83822015005030656 article EN Brazilian Journal of Microbiology 2015-10-24

Gas fermentation has emerged as a sustainable route to produce fuels and chemicals by recycling inexpensive one-carbon (C

10.3389/fbioe.2023.1167892 article EN cc-by Frontiers in Bioengineering and Biotechnology 2023-05-15

Transcriptome analysis via RNA sequencing (RNA-seq) has become a standard technique employed across various biological fields of study. The rapid adoption the RNA-seq approach been mediated, in part, by development different commercial library preparation kits compatible with next-generation (NGS) platforms. Generally, essential steps preparation, such as rRNA depletion and first-strand cDNA synthesis, are tailored to specific group organisms (e.g., eukaryotes versus prokaryotes) or genomic...

10.1128/spectrum.02303-22 article EN Microbiology Spectrum 2022-07-27

The Tetraodontiformes are the most derived group of teleostean fish. Among other apomorphies, they characterized by a high degree fusions or significant bone loss in head and body. In early phylogenetic proposals presented for this order, families Balistidae Monacanthidae have been unanimously considered to be closely related. Although moderate species diversity, scarcely known cytogenetic aspect chromosomal pattern comparisons between these groups yet established. Cantherhines macrocerus...

10.3897/compcytogen.v5i1.1141 article EN cc-by Comparative Cytogenetics 2011-05-05

Resumo: A extensão universitária tem por objetivo instituir práticas cidadãs de pesquisa e ensino. Nesse contexto, o Núcleo Estudos em Microbiologia Agrícola (NEMA), da Universidade Federal Viçosa (UFV), desenvolvido atividades junto à comunidade região, para intercâmbio experiências pesquisa, ensino relacionadas Agrícola. O presente artigo apresenta os primeiros resultados do projeto pelo NEMA Escola Família (EFA) Puris, que adota a Pedagogia Alternância Agroecologia como eixo norteador,...

10.24317/2358-0399.2018v9i2.7576 article ES cc-by Revista Brasileira de Extensão Universitária 2018-08-20

O Acidente Vascular Cerebral (AVC) consiste no comprometimento do suprimento sanguíneo ao cérebro que pode desencadear diversas alterações clínicas, sendo uma das principais causas de morte e incapacitação mundo. No contexto da pandemia COVID-19, os serviços ambulatoriais foram suspensos devido isolamento social, impactando negativamente indivíduos necessitavam intervenções. A telereabilitação foi estratégia assistência fisioterapêutica utilizada para possibilitar a continuidade dos cuidados...

10.21680/2178-6054.2024v17n1id34103 article PT cc-by-nc-sa Revista Extensão & Sociedade 2024-12-27

O presente trabalho cinge-se ao estudo da função socioambiental do Imposto Predial Territorial Urbano (IPTU) e Rural (ITR), desvendando suas correspondentes benesses em favor estruturação de um modelo desenvolvimento socialmente mais justo sustentável.

10.15600/2238-1228/cd.v12n23p193-214 article PT Cadernos de Direito 2012-12-30

ABSTRACT Gas fermentation offers both fossil carbon-free sustainable production of fuels and chemicals recycling gaseous solid waste using gas-fermenting microbes. Bioprocess development, systems-level analysis biocatalyst metabolism, engineering cell factories are advancing the widespread deployment commercialised technology. Acetogens particularly attractive biocatalysts but effects key physiological parameter – specific growth rate (μ) on acetogen metabolism gas bioprocess have not been...

10.1101/2022.02.13.480257 preprint EN bioRxiv (Cold Spring Harbor Laboratory) 2022-02-15

ABSTRACT Gas fermentation has emerged as a sustainable route to produce fuels and chemicals by recycling inexpensive one-carbon (C 1 ) feedstocks from gaseous solid waste using gas-fermenting microbes. Currently, acetogens that utilise the Wood-Ljungdahl pathway convert carbon oxides (CO CO 2 into valuable products are most advanced biocatalysts for gas fermentation. However, our understanding of functionalities genes involved in C -fixing gene cluster its closely-linked is incomplete. Here,...

10.1101/2023.01.30.526161 preprint EN bioRxiv (Cold Spring Harbor Laboratory) 2023-01-30

Abstract Transcriptome analysis via RNA sequencing (RNA-seq) has become a standard technique employed across various biological fields of study. This rapid adoption the RNA-seq approach been mediated, in part, by development different commercial library preparation kits compatible with next-generation (NGS) platforms. Generally, essential steps such as ribosomal (rRNA) depletion and first-strand cDNA synthesis are tailored to specific group organisms (e.g. eukaryotes vs. prokaryotes) or...

10.1101/2022.04.28.489910 preprint EN bioRxiv (Cold Spring Harbor Laboratory) 2022-04-28

Abstract The pandemic caused by the new coronavirus has resulted in a global health emergency and prompted an urgent need for treatment strategies. No target-specific drugs are currently available SARS-CoV-2, but drug candidates targeting viral replication cycle being explored. A prime target of drug-discovery efforts is SARS-CoV-2 main protease (Mpro). In this work, we identified potential inhibitor using silico methodologies. Molecular docking molecular dynamics studies were carried out to...

10.21203/rs.3.rs-672767/v1 preprint EN cc-by Research Square (Research Square) 2021-09-20
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