Daniel M. Snell
A5026871437- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Single-cell and spatial transcriptomics
- Spaceflight effects on biology
- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Gene Regulatory Network Analysis
- Genetics, Aging, and Longevity in Model Organisms
- RNA Research and Splicing
- Animal Virus Infections Studies
- Neuroethics, Human Enhancement, Biomedical Innovations
- Insect Resistance and Genetics
- Neuroinflammation and Neurodegeneration Mechanisms
- COVID-19 Clinical Research Studies
- Intensive Care Unit Cognitive Disorders
- Biosensors and Analytical Detection
- Geriatric Care and Nursing Homes
- Multiple Sclerosis Research Studies
- Viral Infections and Immunology Research
- T-cell and B-cell Immunology
- COVID-19 diagnosis using AI
- Insect symbiosis and bacterial influences
The Francis Crick Institute
2018-2025
Genomics (United Kingdom)
2025
University College London
2013-2020
University College London Hospitals NHS Foundation Trust
2020
National Hospital for Neurology and Neurosurgery
2013-2020
Abstract Missions into Deep Space are planned this decade. Yet the health consequences of exposure to microgravity and galactic cosmic radiation (GCR) over years-long missions on indispensable visceral organs such as kidney largely unexplored. We performed biomolecular (epigenomic, transcriptomic, proteomic, epiproteomic, metabolomic, metagenomic), clinical chemistry (electrolytes, endocrinology, biochemistry) morphometry (histology, 3D imaging, miRNA-ISH, tissue weights) analyses using...
Coronaviruses express their structural and accessory genes via a set of subgenomic RNAs, whose synthesis is directed by transcription regulatory sequences (TRSs) in the 5′ genomic leader upstream each body open reading frame. In SARS-CoV-2, TRS has consensus AAACGAAC; upon searching for emergence this motif global SARS-CoV-2 sequences, we find that it evolves frequently, especially 3′ end genome. We show well-supported examples Spike gene—within nsp16 coding region ORF1b—which expressed...
Axonal degeneration is a major cause of permanent disability in the inflammatory demyelinating disease multiple sclerosis, but no therapies are known to be effective axonal protection. Sodium channel blocking agents can provide protection axons white matter experimental models mechanism action (directly on or indirectly via immune modulation) remains uncertain. Here we have examined efficacy two sodium protect forms autoimmune encephalomyelitis, common model sclerosis. Safinamide currently...
The snapshot nature of single-cell transcriptomics presents a challenge for studying the dynamics cell fate decisions. Metabolic labeling and splicing can provide temporal information at level, but current methods have limitations. Here, we present framework that overcomes these limitations: experimentally, developed sci-FATE2, an optimized method metabolic with increased data quality, which used to profile 45,000 embryonic stem (ES) cells differentiating into neural tube identities....
Abstract Two mutations occurred in SARS-CoV-2 early during the COVID-19 pandemic that have come to define circulating virus lineages 1 : first a change spike protein (D614G) defines B.1 lineage and second, double substitution nucleocapsid (R203K, G204R) B.1.1 lineage, which has subsequently given rise three Variants of Concern: Alpha, Gamma Omicron. While latter appear unremarkable at level, there are dramatic implications nucleotide level: GGG→AAC generates new Transcription Regulatory...
Abstract Human tumors are diverse in their natural history and response to treatment, which part results from genetic transcriptomic heterogeneity. In clinical practice, single-site needle biopsies used sample this diversity, but cancer biomarkers may be confounded by spatiogenomic heterogeneity within individual tumors. Here we investigate clonally expressed genes as a solution the sampling bias problem analyzing multiregion whole-exome RNA sequencing data for 450 tumor regions 184 patients...
Female primordial germ cells (PGCs) undergo X chromosome reactivation (XCR) during genome-wide reprogramming. XCR kinetics and dynamics are poorly understood at a molecular level. Here, we apply single-cell RNA sequencing chromatin profiling on from F
Abstract Animals are essential genetic tools in scientific research and global resources agriculture. In both arenas, a single sex is often required surplus. The ethical financial burden of producing culling animals the undesired considerable. Using mouse as model, we develop synthetic lethal, bicomponent CRISPR-Cas9 strategy that produces male- or female-only litters with one hundred percent efficiency. Strikingly, observe degree litter size compensation relative to control matings,...
Developmental cell fate decisions are dynamic processes driven by the complex behaviour of gene regulatory networks. A challenge in studying these using single-cell genomics is that data provides only a static snapshot with no detail dynamics. Metabolic labelling and splicing can provide time-resolved information, but current methods have limitations. Here, we present experimental computational overcome limitations to allow dynamical modelling expression from data. We developed sci-FATE2, an...
Abstract The emergence of the novel coronavirus SARS-CoV-2 has led to a pandemic infecting more than two million people worldwide in less four months, posing major threat healthcare systems. This is compounded by shortage available tests causing numerous workers unnecessarily self-isolate. We provide roadmap instructing how research institute can be repurposed midst this crisis, collaboration with partner hospitals and an established diagnostic laboratory, harnessing existing expertise virus...
Cognitive Stimulation Therapy (CST, Spector et al., 2006) groups were part of the treatment provided by North Cumbria’s Community Older Adult Service until Covid pandemic halted provision. Subsequently, service to those diagnosed with a dementia relied heavily on pharmacological interventions target rate cognitive decline. This paper documents methodology development project in Cumbria that reintroduced community CST groups, weekly for fourteen weeks across year 2023. Preliminary findings...
Sex chromosomes are emerging as key regulators of adult health and disease in males (XY) females (XX), but their impact on embryo development is poorly understood. Using single-cell RNA sequencing (scRNA-seq) wild type aneuploid mouse embryos, we show that sex significantly shape the preimplantation transcriptional landscape. A hierarchy effects identified, distinctly mediated by Y chromosome, dosage X chromosomes, X-chromosome imprinting, Xist, non-coding initiates X-inactivation. The have...
Abstract Initiating soon after PGC specification, female germ cells undergo reactivation of the silenced X chromosome during genome wide reprogramming. However, kinetics and dynamics XCR in vivo have remained poorly understood. To address this here we perform a global appraisal using high-dimensional techniques. Using F 1 B6 v CAST mouse embryos, detailed assessment, applying single-cell RNA-seq chromatin profiling on purified from E10.5 to E16.5. While scRNA-seq profile showed that male are...
Abstract Animals are extremely useful genetic tools in science and global resources agriculture. However, a single sex is often required surplus, current methods for producing all-female or all-male litters inefficient. Using the mouse as model, we developed synthetic, two-part bicomponent strategy generating litters. We achieved this using CRISPR-Cas9 genome editing technology to generate large stable knock-ins on autosomes X chromosome. The system functions via sex-specific co-inheritance...
<title>Abstract</title> Missions into Deep Space are planned this decade. Yet the health consequences of exposure to microgravity and galactic cosmic radiation (GCR) over years-long missions on indispensable visceral organs such as kidney largely unexplored. We performed biomolecular (epigenomic, transcriptomic, proteomic, epiproteomic, metabolomic, metagenomic), clinical chemistry (electrolytes, endocrinology, biochemistry) morphometry (histology, 3D imaging, miRNA-ISH, tissue weights)...