Wei-Ting Lu
A5015348145- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- SARS-CoV-2 detection and testing
- Lung Cancer Treatments and Mutations
- Genetic factors in colorectal cancer
- Wind and Air Flow Studies
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Biosensors and Analytical Detection
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Fluid Dynamics and Vibration Analysis
- Ferroptosis and cancer prognosis
- Radiomics and Machine Learning in Medical Imaging
- SARS-CoV-2 and COVID-19 Research
- Seismic Performance and Analysis
- Aerodynamics and Fluid Dynamics Research
- Seismic and Structural Analysis of Tall Buildings
- Single-cell and spatial transcriptomics
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
The Francis Crick Institute
2019-2025
Xiangya Hospital Central South University
2025
Central South University
2025
Hainan Medical University
2025
University of Florida
2022-2024
The University of Texas MD Anderson Cancer Center
2022-2024
Ocean University of China
2006-2024
Changshu No.1 People's Hospital
2022
Kaohsiung Chang Gung Memorial Hospital
2022
Chang Gung University
2022
MicroRNAs (miRNAs) control gene expression through both translational repression and degradation of target messenger RNAs (mRNAs). However, the interplay between these processes precise molecular mechanisms involved remain unclear. Here, we show that inhibition is primary event required for mRNA degradation. Translational depends on miRNAs impairing function eIF4F initiation complex. We define RNA helicase eIF4A2 as key factor which function. uncover a correlation presence miRNA sites in 3'...
Abstract The error-free and efficient repair of DNA double-stranded breaks (DSBs) is extremely important for cell survival. RNA has been implicated in the resolution damage but mechanism remains poorly understood. Here, we show that miRNA biogenesis enzymes, Drosha Dicer, control recruitment factors from multiple pathways to sites damage. Depletion significantly reduces by both homologous recombination (HR) non-homologous end joining (NHEJ). required within minutes break induction,...
Abstract In this study, the impact of apolipoprotein B mRNA-editing catalytic subunit-like (APOBEC) enzyme APOBEC3B (A3B) on epidermal growth factor receptor (EGFR)-driven lung cancer was assessed. A3B expression in EGFR mutant (EGFRmut) non-small-cell (NSCLC) mouse models constrained tumorigenesis, while tumors treated with EGFR-targeted therapy associated treatment resistance. Analyses human NSCLC showed upregulation and revealed therapy-induced activation nuclear kappa (NF-κB) as an...
Abstract APOBEC3 enzymes are cytosine deaminases implicated in cancer. Precisely when expression is induced during cancer development remains to be defined. Here we show that specific genes upregulated breast ductal carcinoma situ, and preinvasive lung lesions coincident with cellular proliferation. We observe evidence of APOBEC3-mediated subclonal mutagenesis propagated from TRACERx invasive non–small cell (NSCLC) lesions. find APOBEC3B exacerbates DNA replication stress chromosomal...
Extrachromosomal DNA (ecDNA) is a major contributor to treatment resistance and poor outcome for patients with cancer
Patients are turning to OHCs deal with the stresses and complications of infertility. As a stigmatized disease, infertile patients may value informational support emotional differently, which is ignored in existing studies. Based on social theory, this study aims reveal role doctors patient decision-making. We select Haodf.com as our data source we collected information for all new 2,989 doctors. use Jieba word segmentation tool TF-IDF algorithm segment find keywords. Then random forest used...
Abstract A key player in translation initiation is eIF4E, the mRNA 5′ cap-binding protein. 4E-Transporter (4E-T) a recently characterized eIF4E-binding protein, which regulates specific mRNAs several developmental model systems. Here, we first investigated role of its enrichment P-bodies and translational regulation mammalian cells. Identification conserved C-terminal sequences that target 4E-T to was enabled by comparison vertebrate proteins with homologues Drosophila (Cup CG32016)...
Abstract Background Regulation of the mRNA life cycle is central to gene expression control and determination cell fate. miRNAs represent a critical regulatory mechanism, but despite decades research, their mode action still not fully understood. Results Here, we show that eIF4A2 major effector repressive miRNA pathway functioning via Ccr4-Not complex. We demonstrate while DDX6 interacts with Ccr4-Not, its effects in mechanism are as pronounced. Through interaction complex, represses mRNAs...
Summary We constructed the microsatellite‐based linkage maps using 318 markers typed in two F 1 outbred families of Zhikong scallop ( Chlamys farreri ). The results showed an extremely high proportion (56.2%) non‐amplifying null alleles and a ratio (30%) segregation distortion. By aligning different individual‐based maps, 19 groups were identified, which are consistent with haploid chromosome number scallop. integrated map contains 154 covering 1561.8 cM average intermarker spacing 12.3...
Undifferentiated pleomorphic sarcoma (UPS) can be associated with a relatively dense immune infiltration. Immune checkpoint inhibitors (anti-PD1, anti-PDL1, and anti-CTLA4) are effective in 20% of UPS patients. We characterize the microenvironment its association oncologic outcomes.Surgically resected samples were stained by immunohistochemistry (IHC) for following: tumor-associated cells (CD3, CD8, CD163, CD20), checkpoints (stimulatory: OX40, ICOS; inhibitory: PD-L1, LAG3, IDO1, PD1),...
Abstract During each cell cycle, the process of DNA replication timing is tightly regulated to ensure accurate duplication genome. The extent and significance alterations in this during malignant transformation have not been extensively explored. Here, we assess impact altered (ART) on cancer evolution by analysing replication-timing sequencing normal lines 952 whole-genome sequenced lung breast tumours. We find that 6%–18% genome exhibits ART, with regions a change from early late...
The integration of biomechanical and morphological analyses holds tremendous potential for assessing the rupture risk abdominal aortic aneurysms (AAA). We employed a one-way fluid-structure interaction (FSI) model to distinguish between ruptured AAA (RAAA) asymptomatic intact (IAAA), focusing on computational fluid dynamics (CFD) indices. Patient groups with RAAA IAAA were matched by diameter, age, sex. morphology was analyzed via CT segmentation, indices—including wall shear stress (WSS),...
Abstract The integrity of the genome is maintained by a host surveillance and repair mechanisms that are pivotal for cellular function. tumour suppressor protein p53 major component DNA damage response pathway plays vital role in maintenance cell-cycle checkpoints. Here we show microRNA, miR-486, its gene ankyrin-1 ( ANK1 ) induced following damage. Strikingly, cytoskeleton adaptor was over 80-fold upregulated to variety agents range cell types. We demonstrate miR-486-5p involved controlling...
DNA methylation is an important way to regulate gene expression in eukaryotes. In order reveal the role of regulation germ cell-specific piwi during spermatogenesis Japanese flounder (Paralichthys olivaceus), profiles piwil1 (piwi-like 1) and piwil2 2) genes gonads female, male, sex-reversed pseudo-male P. olivaceus were analyzed, dynamic was investigated. As a result, highly expressed testis both male olivaceus, with significant variation among individuals. The levels promoter regions...
Abstract Human tumors are diverse in their natural history and response to treatment, which part results from genetic transcriptomic heterogeneity. In clinical practice, single-site needle biopsies used sample this diversity, but cancer biomarkers may be confounded by spatiogenomic heterogeneity within individual tumors. Here we investigate clonally expressed genes as a solution the sampling bias problem analyzing multiregion whole-exome RNA sequencing data for 450 tumor regions 184 patients...
Abstract Background Today, the prevalence of bronchiectasis is on rise worldwide and ranks third among chronic respiratory diseases, yet there currently no standard treatment. Exploring new drug targets based a patient’s genetic profile provides directions to further address current challenges bronchiectasis. Methods First, we used cis-expression quantitative trait locus (cis-eQTL) data druggable genes from blood samples as exposure factor genome-wide association study (GWAS) outcome perform...
Abstract Background Chromosomal instability (CIN) is pervasive during cancer evolution, particularly in non-small cell lung (NSCLC) where it associated with poor recurrence-free survival and correlates the frequency of whole genome doubling events (WGD). By duplicating complete set chromosomes, WGD a key event evolution prognosis targeted therapy resistance. Despite importance these events, genetic responsible for initiation maintenance CIN NSCLC have not been systematically investigated....
<ns4:p>The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. The vast majority patient samples is assessed viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals an accredited clinical diagnostic laboratory, established a testing pipeline that has reported on more than 252,000 RT-qPCR results since its commencement at the beginning April 2020. However, due demand...