Laura Cubitt
A5017568396- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- SARS-CoV-2 and COVID-19 Research
- Advanced biosensing and bioanalysis techniques
- COVID-19 Clinical Research Studies
- COVID-19 and healthcare impacts
- Cancer-related molecular mechanisms research
- Spaceflight effects on biology
- Single-cell and spatial transcriptomics
- COVID-19 diagnosis using AI
- Molecular Biology Techniques and Applications
- Genetic factors in colorectal cancer
- Cancer, Hypoxia, and Metabolism
- IL-33, ST2, and ILC Pathways
- Family and Patient Care in Intensive Care Units
- Climate Change and Health Impacts
- COVID-19 epidemiological studies
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Genetics, Aging, and Longevity in Model Organisms
- RNA modifications and cancer
- Folate and B Vitamins Research
- Inflammatory Bowel Disease
- Genetic Associations and Epidemiology
- Geriatric Care and Nursing Homes
The Francis Crick Institute
2020-2024
University College London
2020
University College London Hospitals NHS Foundation Trust
2020
National Hospital for Neurology and Neurosurgery
2020
Royal Marsden Hospital
2020
Centre for Human Genetics
2017-2018
University of Oxford
2017-2018
University of Edinburgh
2015
Highlights•Single-cell census of the colonic mesenchyme reveals unexpected heterogeneity•Identification crypt niche mesenchymal cell expressing SOX6 and Wnts•Definition fundamental aspects remodeling in colitis•Analysis colitis-associated cells pathogenicity driversSummaryIntestinal play essential roles epithelial homeostasis, matrix remodeling, immunity, inflammation. But extent heterogeneity within these processes remains unknown. Using unbiased single-cell profiling over 16,500 cells, we...
Abstract Intratumour heterogeneity (ITH) fuels lung cancer evolution, which leads to immune evasion and resistance therapy 1 . Here, using paired whole-exome RNA sequencing data, we investigate intratumour transcriptomic diversity in 354 non-small cell tumours from 347 out of the first 421 patients prospectively recruited into TRACERx study 2,3 Analyses 947 tumour regions, representing both primary metastatic disease, alongside 96 tumour-adjacent normal tissue samples implicate transcriptome...
Patients with cancer have higher COVID-19 morbidity and mortality. Here we present the prospective CAPTURE study, integrating longitudinal immune profiling clinical annotation. Of 357 patients cancer, 118 were SARS-CoV-2 positive, 94 symptomatic 2 died of COVID-19. In this cohort, 83% had S1-reactive antibodies 82% neutralizing against wild type SARS-CoV-2, whereas antibody titers Alpha, Beta Delta variants substantially reduced. levels decreased in 13% patients, remained stable for up to...
Abstract Missions into Deep Space are planned this decade. Yet the health consequences of exposure to microgravity and galactic cosmic radiation (GCR) over years-long missions on indispensable visceral organs such as kidney largely unexplored. We performed biomolecular (epigenomic, transcriptomic, proteomic, epiproteomic, metabolomic, metagenomic), clinical chemistry (electrolytes, endocrinology, biochemistry) morphometry (histology, 3D imaging, miRNA-ISH, tissue weights) analyses using...
Abstract The design and implementation of single-cell experiments is often limited by their requirement for fresh starting material. We have adapted a method histological tissue fixation using dithio-bis(succinimidyl propionate) (DSP), or Lomant’s Reagent, to stabilise cell samples transcriptomic applications. DSP reversible cross-linker free amine groups that has previously been shown preserve integrity histology while maintaining RNA yield in bulk extractions. Although RNA-seq data from...
Abstract To maximize the impact of precision medicine approaches, it is critical to identify genetic variants underlying disease and accurately quantify their functional effects. A gene exemplifying challenge variant interpretation von Hippel–Lindautumor suppressor ( VHL ). encodes an E3 ubiquitin ligase that regulates cellular response hypoxia. Germline pathogenic in predispose patients tumors including clear cell renal carcinoma (ccRCC) pheochromocytoma, somatic mutations are frequently...
<ns4:p>The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. The vast majority patient samples is assessed viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals an accredited clinical diagnostic laboratory, established a testing pipeline that has reported on more than 252,000 RT-qPCR results since its commencement at the beginning April 2020. However, due demand...
<ns4:p>The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. The vast majority patient samples is assessed viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals an accredited clinical diagnostic laboratory, established a testing pipeline that has reported on more than 252,000 RT-qPCR results since its commencement at the beginning April 2020. However, due demand...
Abstract The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. vast majority patient samples is assessed viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals an accredited clinical diagnostic laboratory, established a testing pipeline that has reported on more than 40,000 RT-qPCR results since its commencement at the beginning April 2020. However, due demand...
Abstract The emergence of the novel coronavirus SARS-CoV-2 has led to a pandemic infecting more than two million people worldwide in less four months, posing major threat healthcare systems. This is compounded by shortage available tests causing numerous workers unnecessarily self-isolate. We provide roadmap instructing how research institute can be repurposed midst this crisis, collaboration with partner hospitals and an established diagnostic laboratory, harnessing existing expertise virus...
Patients with cancer have higher COVID-19 morbidity and mortality. Here we present the prospective CAPTURE study (NCT03226886) integrating longitudinal immune profiling clinical annotation. Of 357 patients cancer, 118 were SARS-CoV-2-positive, 94 symptomatic 2 died of COVID-19. In this cohort, 83% had S1-reactive antibodies, 82% neutralizing antibodies against WT, whereas antibody titers (NAbT) Alpha, Beta, Delta variants substantially reduced. Whereas levels decreased in 13% patients, NAbT...
Despite major advances in spatial RNA sequencing, the ability to extract temporal information sequencing experiments is still limited. Here, we describe Transcriptome Timestamping (T2), a system which harnesses naturally occurring A-to-I editing of transcripts unmodified human cells infer transcriptional history. T2 provides age estimates for individual transcripts, and serves as an endogenous molecular recorder, differentiating between complex programs. We show that can identify transient...
Abstract The design and implementation of single-cell experiments is often limited by their requirement for fresh starting material. We have adapted a method histological tissue fixation using dithio-bis(succinimidyl propionate) (DSP), or Lomant’s Reagent, to stabilise cell samples transcriptomic applications. DSP reversible cross-linker free amine groups that has previously been shown preserve integrity histology while maintaining RNA yield in bulk extractions. Although RNA-seq data from...
Purpose of examination / Clinical relevance At the end 2019, several pneumonia cases were reported in Wuhan, China and pathogen was confirmed as a new viral strain. World Health organization has named newly identified coronavirus 2019-nCoV, also known SARS-Cov-2. The disease developed into dangerous pandemic, posing major challenges to NHS. Although more research is necessary better understand virus, response emergency, simple rapid testing essential identify virus infected individuals. This...
<title>Abstract</title> Missions into Deep Space are planned this decade. Yet the health consequences of exposure to microgravity and galactic cosmic radiation (GCR) over years-long missions on indispensable visceral organs such as kidney largely unexplored. We performed biomolecular (epigenomic, transcriptomic, proteomic, epiproteomic, metabolomic, metagenomic), clinical chemistry (electrolytes, endocrinology, biochemistry) morphometry (histology, 3D imaging, miRNA-ISH, tissue weights)...
Purpose of examination / Clinical relevance At the end 2019, several pneumonia cases were reported in Wuhan, China and pathogen was confirmed as a new viral strain. World Health organization has named newly identified coronavirus 2019-nCoV, also known SARS-Cov-2. The disease developed into dangerous pandemic, posing major challenges to NHS. Although more research is necessary better understand virus, response emergency, simple rapid testing essential identify virus infected individuals. This...