A5009088784- Substance Abuse Treatment and Outcomes
- Smoking Behavior and Cessation
- Prenatal Substance Exposure Effects
- Autoimmune and Inflammatory Disorders Research
- Liver Disease Diagnosis and Treatment
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- American Constitutional Law and Politics
- Diet and metabolism studies
- Inflammatory Myopathies and Dermatomyositis
- Cholesterol and Lipid Metabolism
- Immunodeficiency and Autoimmune Disorders
- Nerve injury and regeneration
- Transcranial Magnetic Stimulation Studies
- Spaceflight effects on biology
- Nutritional Studies and Diet
- Opioid Use Disorder Treatment
- Folate and B Vitamins Research
- Nerve Injury and Rehabilitation
- Diet, Metabolism, and Disease
- Religion and Society Interactions
- Cutaneous Melanoma Detection and Management
- Neuroscience and Neuropharmacology Research
- Cardiac electrophysiology and arrhythmias
- Nuclear Receptors and Signaling
Hunter New England Local Health District
2015-2024
University of North Carolina at Chapel Hill
2024
Texas Children's Hospital
2017-2024
Baylor College of Medicine
2018-2024
Hunter Medical Research Institute
2009-2023
University of Newcastle Australia
2009-2023
Arkansas Children's Hospital
2023
Southern Illinois University School of Medicine
2021-2023
University of Arkansas for Medical Sciences
2023
University of Minnesota, Duluth
2023
Abstract Missions into Deep Space are planned this decade. Yet the health consequences of exposure to microgravity and galactic cosmic radiation (GCR) over years-long missions on indispensable visceral organs such as kidney largely unexplored. We performed biomolecular (epigenomic, transcriptomic, proteomic, epiproteomic, metabolomic, metagenomic), clinical chemistry (electrolytes, endocrinology, biochemistry) morphometry (histology, 3D imaging, miRNA-ISH, tissue weights) analyses using...
The prognosis of children with juvenile dermatomyositis (JDM) has improved remarkably since the 1960's use corticosteroid and immunosuppressive therapy. Yet there remain a minority who have refractory disease. Since 2003 sporadic biologics (genetically-engineered proteins that usually are derived from human genes) for inflammatory myositis been reported. In 2011-2016 we investigated our collective experience in JDM through Childhood Arthritis Rheumatology Research Alliance (CARRA).The...
Abstract Background A better understanding of the pathogenesis polyarticular juvenile idiopathic arthritis (polyJIA) is needed to aide in development data-driven approaches guide selection between therapeutic options. One inflammatory pathway interest JAK-STAT signaling. STAT3 a transcription factor critical differentiation T helper 17 cells (Th17s). Previous studies have demonstrated increased activation adult patients with rheumatoid arthritis, but less known about polyJIA. We hypothesized...
Abstract MicroRNAs (miRNAs) within the ventral and dorsal striatum have been shown to regulate addiction‐relevant behaviours. However, it is unclear how cocaine experience alone can alter expression of miRNAs striatal subregions. Further, not known whether differential in contributes individual differences addiction vulnerability. We first examined effect self‐administration on miR‐101b, miR‐137, miR‐212 miR‐132 nucleus accumbens core shell (NAcSh), as well dorsomedial dorsolateral (DLS)....
Recently, we published data using an animal model that allowed us to characterize animals into two groups, addiction vulnerable and resilient, where identified addiction/relapse vulnerability was associated with deficits in synaptic plasticity-associated gene expression the dorsal striatum (DS). Notable strong reduction for activity-regulated cytoskeleton-associated protein (Arc) considered a master regulator of plasticity. In present study, confirmed Arc messenger RNA significantly...
Methadone is a widely used opioid agonist treatment associated with QT prolongation and torsades de pointes. We investigated the interval in patients treated methadone or buprenorphine using continuous 12-lead Holter recordings.
Access to opioid agonist treatment can be associated with extensive waiting periods significant health and financial burdens. This study aimed determine whether patients heroin dependence dispensed buprenorphine-naloxone weekly have greater reductions in use related adverse effects 12-weeks after commencing treatment, compared waitlist controls examine the cost-effectiveness of this strategy. An open-label RCT was conducted an clinic Newcastle, Australia. Fifty DSM-IV-TR (and no other...
As technology rapidly advances, the evidence for efficacy of brain-based therapeutics, such as repetitive transcranial magnetic stimulation (rTMS), treatment numerous neurological and neuropsychiatric conditions continues to grow. Indeed, promising therapeutic results date have led several international regulatory bodies approving use rTMS depression, tobacco smoking cessation, migraines obsessive-compulsive disorder. This is encouraging, could be successfully expanded treat other...
Reducing the likelihood of relapse represents one greatest obstacles in successful treatment cocaine addiction. Dysregulation synaptic plasticity processes long-term potentiation (LTP) and depression (LTD) is thought to be associated with protracted risk. To improve our understanding molecular mechanisms contributing vulnerability we trained rats (n=52) self-administer phenotyped animals as relapse-vulnerable or relapse-resilient using procedures adapted from Deroche-Gamonet et al. (Science...
Transplantation studies suggest that bone marrow cell ATP-binding cassette transporter A1 protects against atherosclerosis development. However, the in vivo effect of macrophage expression on atherogenesis is not fully understood because contains other leukocytes and hematopoietic stem progenitor cells. Myeloid-specific knockout mice low-density lipoprotein (LDL) receptor C57BL/6 background were developed to address this question.Chow-fed myeloid-specific knockout/LDL (double [DKO]) versus...
Background Referral for brief intervention among people who misuse alcohol is reported to be effective but its impact those present services following deliberate self-harm (DSH) has not been examined. Method Consecutive patients presented an Emergency Department (ED) episode of DSH were screened misuse. Those found misusing randomly assigned plus a health information leaflet or alone. The primary outcome was whether the patient reattended ED further during subsequent 6 months. Secondary...
Microdissection techniques have the potential to allow for transcriptome analyses in specific populations of cells that are isolated from heterogeneous tissues such as nervous system and certain cancers. Problematically, RNA is not stable under labeling conditions usually needed identify interest microdissection. We developed an immunolabeling method utilizes a high salt buffer stabilize during prolonged antibody incubations. first assessed integrity by three methods found tissue incubated...
BACKGROUND Routine collection of patient-reported outcomes is needed to better understand recovery, benchmark between trauma centers and systems, monitor over time. A key component follow-up methodology the mode administration outcome measures with multiple options available. We aimed quantify patient preference compare response rates data completeness for telephone online completion in patients. METHODS registry-based cohort study adult (16 years older) patients registered Victorian State...
Abstract Background Despite new and better treatments for juvenile dermatomyositis (JDM), not all patients with moderate severity disease respond adequately to first-line therapy. Those refractory remain at higher risk glucocorticoid-related complications. Biologic disease-modifying antirheumatic drugs (DMARDs) have become part of the arsenal JDM. However, prospective comparative studies commonly used biologics are lacking. Methods The Childhood Arthritis Rheumatology Research Alliance...
The ability to microdissect individual cells from the nervous system has enormous potential, as it can allow for study of gene expression in phenotypically identified cells. However, if resultant profiles are be accurately ascribed, is necessary determine extent contamination by nontarget microdissected sample. Here, we show that midbrain dopamine neurons laser-microdissected a high degree enrichment and purity. average tyrosine hydroxylase (TH) sample relative sections was approximately...
Obesity-associated low-grade chronic inflammation plays an important role in the development of insulin resistance. The membrane lipid transporter ATP-binding cassette A1 (ABCA1) promotes formation nascent HDL particles. ABCA1 also dampens macrophage by reducing cellular cholesterol and raft content. We tested hypothesis that myeloid-specific deletion may exacerbate resistance increasing obesity-associated inflammation. Myeloid cell-specific knockout (MSKO) wild-type (WT) mice developed...
Abstract Introduction and Aims Drug induced QT prolongation occurs in patients with substance use disorders from prescription medications that prolong the QT, such as methadone. Knowing prevalence of this population is important for prescribers. This study aimed to investigate current disorders. Design Methods We undertook a retrospective review electrocardiograms (ECG) an urban general hospital large drug alcohol service toxicology unit. ECGs were taken seen by unit over three years. The...