A5089918136- Adipokines, Inflammation, and Metabolic Diseases
- Peroxisome Proliferator-Activated Receptors
- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Fatty Acid Research and Health
- Immune cells in cancer
- Cholesterol and Lipid Metabolism
- Phytochemicals and Antioxidant Activities
- Genetic Mapping and Diversity in Plants and Animals
- Genetic and phenotypic traits in livestock
- Liver Disease Diagnosis and Treatment
- Antioxidant Activity and Oxidative Stress
- Lipid metabolism and biosynthesis
- Natural Compound Pharmacology Studies
- Immune Response and Inflammation
- Drug Transport and Resistance Mechanisms
- Phagocytosis and Immune Regulation
- Sirtuins and Resveratrol in Medicine
- Pancreatic function and diabetes
- Neurobiology and Insect Physiology Research
- Neutropenia and Cancer Infections
- Gut microbiota and health
- Endoplasmic Reticulum Stress and Disease
- Intensive Care Unit Cognitive Disorders
- Ginger and Zingiberaceae research
National Taiwan University
2006-2025
Wake Forest University
2016-2025
National Taiwan University Hospital
2007-2024
Icahn School of Medicine at Mount Sinai
2018
University of Nebraska–Lincoln
2018
Winston-Salem State University
2018
Taipei Veterans General Hospital
2017
Winston-Salem/Forsyth County Schools
2015
University of North Carolina at Greensboro
2008-2013
Greensboro College
2013
Obesity is linked to type 2 diabetes (T2D) and cardiovascular diseases; however, the underlying molecular mechanisms remain unclear. We aimed identify obesity-associated features that may contribute obesity-related diseases. Using circulating monocytes from 1,264 Multi-Ethnic Study of Atherosclerosis (MESA) participants, we quantified transcriptome epigenome. discovered alterations in a network coexpressed cholesterol metabolism genes are signature feature obesity inflammatory stress. This...
Dietary PUFAs reduce atherosclerosis and macrophage inflammation, but how nucleotide-binding oligomerization domain leucine-rich repeat-containing receptor protein (NLRP3) inflammasome activation autophagy influence PUFA-mediated atheroprotection is poorly understood. We fed Ldlr-/- mice diets containing 10% (calories) palm oil (PO) 0.2% cholesterol, supplemented with an additional of calories as PO, fish (FO), echium (EO, 18:4 n-3), or borage (BO, 18:3 n-6). Inflammasome activation,...
Metabolic reprogramming between resistance and tolerance occurs within the immune system in response to sepsis. While metabolic tissues such as liver are subjected damage during sepsis, how their energy ensures survival is unclear. Employing comprehensive metabolomic, lipidomic, transcriptional profiling a mouse model of we show that hepatocyte lipid metabolism, mitochondrial tricarboxylic acid (TCA) energetics, redox balance significantly reprogrammed after cecal ligation puncture (CLP). We...
The objective of this study was to examine the mechanism by which conjugated linoleic acid (CLA) reduces body fat. Young male mice were fed three combinations fatty acids at doses (0.06%, 0.2%, and 0.6%, w/w) incorporated into AIN76 diets for 7 weeks. types (control), an equal mixture trans-10, cis-12 (10,12) CLA plus acid, isomer 10,12 cis-9, trans-11 (9,11) CLA. Mice receiving 0.2% 0.6% dose or had decreased white adipose tissue (WAT) brown (BAT) mass increased incorporation isomers in...
The molecular events of hyperglycemia-triggered increase in adipogenic induction lipid accumulation remain unclear. We examined the effects hyperglycemia on and its involved signaling molecules, such as phosphoinositide 3-kinase (PI3K), ERKs, peroxisome proliferator-activated receptor gamma (PPAR gamma). Bone marrow-derived mesenchymal stem cells (MSCs) isolated from FVB/N mice were capable differentiating into adipocytes medium. high glucose (HG) (25.5 mm) assessed vitro by RT-PCR, ELISA,...
Inflammation plays a role in <i>trans</i>-10, <i>cis</i>-12 (10,12)-conjugated linoleic acid (CLA)-mediated delipidation and insulin resistance adipocytes. Given the anti-inflammatory of resveratrol (RSV), we hypothesized that RSV would attenuate inflammation caused by 10,12 CLA human blocked induction inflammatory response preventing activation extracellular signal-related kinase gene expression (i.e., IL-6, IL-8, IL-1β) within 12 h. Similarly, suppressed CLA-mediated prostaglandin pathway...
Adipose tissue cholesterol increases with adipocyte triglyceride content and size during development of obesity. However, how affects function is poorly understood. The aim this study was to evaluate the role cellular exporter, Abca1 (ATP-binding cassette transporter A1), on adipose diet-induced obesity.Adiponectin Cre recombinase transgenic mice were crossed Abca1flox/flox generate ASKO (adipocyte-specific knockout) mice. Control then fed a high-fat, high-cholesterol (45% calories as fat...
Obesity is a major risk factor for multiple chronic diseases. Anthropometric and imaging approaches are primarily used to assess adiposity, there dearth of techniques determine the changes in adipose tissue (AT) at molecular level. Extracellular vesicles (EVs) have emerged as novel less invasive source biomarkers various pathologies. Furthermore, possibility enriching cell or tissue-specific EVs from biofluids based on their unique surface markers has led classifying these "liquid biopsies",...
Abstract Background It remains unclear whether interleukin (IL) 6 plays a role in initiating either the inflammatory or antiapoptotic responses severe acute pancreatitis. This study examined effect of neutralizing antibody against IL-6 on induction pancreatic acinar cell apoptosis and attenuation severity Methods Experiments were conducted laboratory mice with pancreatitis induced by lipopolysaccharide injection following six injections caerulein at intervals h. Neutralizing monoclonal...
The objective of this study was to determine the anti-inflammatory properties grape powder (GP) or GP extract (GE) and examine (1) which polyphenol metabolites in GE were bioavailable, (2) impact on glucose tolerance inflammation obese mice, (3) if bioavailable polyphenols decrease markers primary adipocytes. In experiment 1, C57BL/6J mice gavaged with serum measured. 2, fed high-fat diets supplemented 3% 0.02% for 18 weeks 3, human adipocytes treated quercetin 3-O-glucoside (Q3G)...
Plasma membrane (PM) free cholesterol (FC) is emerging as an important modulator of signal transduction. Here, we show that hepatocyte-specific knockout (HSKO) the cellular FC exporter, ATP-binding cassette transporter A1 (ABCA1), leads to decreased PM content and defective trafficking lysosomal PM. Compared with controls, chow-fed HSKO mice had reduced hepatic (1) insulin-stimulated Akt phosphorylation, (2) activation lipogenic transcription factor Sterol Regulatory Element Binding Protein...
One primary metabolic manifestation of inflammation is the diversion cis-aconitate within tricarboxylic acid (TCA) cycle to synthesize immunometabolite itaconate. Itaconate well established possess immunomodulatory and effects myeloid cells lymphocytes, however, its in other organ systems during sepsis remain less clear. Utilizing Acod1 knockout mice that are deficient synthesizing itaconate, we aimed understand role itaconate liver systemically sepsis. We find aids lipid metabolism...
Abstract Induced pluripotent stem cell-derived neural progenitor cells (iPSC-NPCs) are a promising source of tailor-made cell therapy for neurological diseases. However, major obstacles to clinical use still exist. To circumvent complications related intracerebral administration, we implanted human iPSC-NPCs epidurally over the peri-infarct cortex 7 days after permanent middle cerebral artery occlusion in adult rats. Compared controls, cell-treated rats showed significant improvements...
The role of hepatocyte Abca1 (ATP binding cassette transporter A1) in trafficking hepatic free cholesterol (FC) into plasma versus bile for reverse transport (RCT) is poorly understood. We hypothesized that recycles HDL-C (high-density lipoprotein cholesterol) taken up by the liver back plasma, maintaining pool, and decreasing HDL-mediated RCT feces. Approach Results: Chow-fed hepatocyte-specific knockout (HSKO) control mice were injected with human HDL radiolabeled 125I-tyramine cellobiose...