A5042974889- Cell Adhesion Molecules Research
- Platelet Disorders and Treatments
- Protease and Inhibitor Mechanisms
- Blood properties and coagulation
- Blood Coagulation and Thrombosis Mechanisms
- Caveolin-1 and cellular processes
- Angiogenesis and VEGF in Cancer
- Antiplatelet Therapy and Cardiovascular Diseases
- Monoclonal and Polyclonal Antibodies Research
- Biochemical and Structural Characterization
- Cellular Mechanics and Interactions
- Heparin-Induced Thrombocytopenia and Thrombosis
- Signaling Pathways in Disease
- S100 Proteins and Annexins
- Peptidase Inhibition and Analysis
- Immune Response and Inflammation
- Immune cells in cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Erythrocyte Function and Pathophysiology
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Blood disorders and treatments
- Glycosylation and Glycoproteins Research
- Atherosclerosis and Cardiovascular Diseases
- Acute Myocardial Infarction Research
- Fibroblast Growth Factor Research
Cleveland Clinic
2012-2024
Cleveland Clinic Lerner College of Medicine
2015-2024
The Wistar Institute
2022
Jacobs (United States)
2008-2019
Case Comprehensive Cancer Center
1998-2017
Cleveland Eye Clinic
2015
Shanghai Blood Center
2014
Shanghai Jiao Tong University
2014
Shanghai University
2014
Versiti Blood Center of Wisconsin
2014
Adhesive interactions of the platelet surface with plasma proteins such as fibrinogen and fibronectin play an important role in thrombosis hemostasis. The binding both these to platelets is inhibited by synthetic peptides containing sequence Arg-Gly-Asp, which corresponds cell adhesion site also present alpha chain fibrinogen. An affinity matrix made insolubilized heptapeptide Arg-Gly-Asp selectively binds membrane glycoprotein IIb/IIIa from detergent extracts platelets. When incorporated...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRole of cell-surface lysines in plasminogen binding to cells: identification .alpha.-enolase as a candidate receptorLindsey A. Miles, Carol M. Dahlberg, Janet Plescia, Jordi Felez, Kanefusa Kato, and Edward F. PlowCite this: Biochemistry 1991, 30, 6, 1682–1691Publication Date (Print):February 12, 1991Publication History Published online1 May 2002Published inissue 12 February...
The ligand-binding function of integrin adhesion receptors depends on divalent cations. A mutant α IIb β 3 (platelet gpIIb/IIIa) that lacks ligand recognition shows immunologic evidence a perturbed interaction with This was found to be caused by G → T mutation resulted in an Asp 119 Tyr substitution the subunit. residue is proximal bound and conserved region among integrins are enriched oxygenated residues. spacing these residues aligns calcium-binding EF hand proteins, suggesting...
The leukocyte integrin alpha(M)beta(2)/Mac-1 appears to support the inflammatory response through multiple ligands, but local engagement of fibrin(ogen) may be particularly important for function. To define biological significance fibrin(ogen)-alpha(M)beta(2) interaction in vivo, gene-targeted mice were generated which alpha(M)beta(2)-binding motif within fibrinogen gamma chain (N(390)RLSIGE(396)) was converted a series alanine residues. Mice carrying Fibgamma(390-396A) allele maintained...
Many adhesive interactions are mediated by Arg-Gly-Asp (RGD) sequences within proteins. Such RGD frequently recognized structurally related heterodimers that members of the integrin family adhesion receptors. A region was found in platelet receptor, gpIIb/IIIa, to which an peptide becomes chemically cross-linked. This corresponds residues 109 171 gpIIIa. segment is conserved among β subunits integrins (76 percent identity sequence), indicating it may play a role functions this
Background Circumstantial evidence suggests that the plasminogen/plasmin system plays a role in many biological processes, including hemostasis, cell migration, and development. Methods Results The vivo function of was studied by generation plasminogen-deficient ( Plg −/− ) mice. Inactivation murine plasminogen gene achieved replacing, via homologous recombination embryonic stem cells, genomic sequences encoding exons containing catalytic site amino acids His 605 Asp 648 with neomycin...
Occupancy of integrin receptors induces conformational changes in the receptor, resulting exposure novel interactive sites termed ligand-induced binding (LIBS). We report here that Fab fragments certain antibodies against LIBS on alpha IIb beta 3 (platelet glycoprotein IIb-IIIa) block platelet aggregation. Thus, or regions surrounding them may participate events required for In addition, anti-alpha stimulated This was due to induction fg 3, apparently by shifting a equilibrium between...
Abstract Background microRNAs have been established as powerful regulators of gene expression in normal physiological well pathological conditions, including cancer progression and metastasis. Recent studies demonstrated a key role miR-31 the metastasis breast cancer. Downregulation enhances several steps invasion-metastasis cascade cancer, i.e., local invasion, extravasation survival circulation system, metastatic colonization distant sites. exerts its metastasis-suppressor activity by...
Integrin activation is essential for dynamically linking the extracellular environment and cytoskeletal/signaling networks. Activation controlled by integrins' short cytoplasmic tails (CTs). It widely accepted that head domain of talin (talin-H) can mediate integrin binding to two sites in β's CT; β3 this an NPLY747 motif membrane-proximal region. Here, we show C-terminal region CT, composed a conserved TS752T NITY759 motif, supports cytosolic partner, kindlin-2, distributed PTB protein....
The functional responses of endothelial cells are dependent on signaling from peptide growth factors and the cellular adhesion receptors, integrins. These include cell adhesion, migration, proliferation, which, in turn, essential for more complex processes such as formation tube network during angiogenesis. This study identifies molecular requirements cross-activation between beta3 integrin tyrosine kinase receptor 2 vascular factor (VEGF) (VEGFR-2) endothelium. relationship VEGFR-2 appears...
Myeloperoxidase (MPO) and paraoxonase 1 (PON1) are high-density lipoprotein-associated (HDL-associated) proteins mechanistically linked to inflammation, oxidant stress, atherosclerosis. MPO is a source of ROS during inflammation can oxidize apolipoprotein A1 (APOA1) HDL, impairing its atheroprotective functions. In contrast, PON1 fosters systemic antioxidant effects promotes some the properties attributed HDL. Here, we demonstrate that MPO, PON1, HDL bind one another, forming ternary...
Abstract Talin-induced integrin binding to extracellular matrix ligands (integrin activation) is the key step trigger many fundamental cellular processes including cell adhesion, migration, and spreading. Talin widely known use its N-terminal head domain (talin-H) bind activate integrin, but how talin-H operates in context of full-length talin surrounding remains unknown. Here we show that while being capable inducing activation, alone exhibits unexpectedly low potency versus a...