A5051682378- Gut microbiota and health
- Diet and metabolism studies
- Liver Disease Diagnosis and Treatment
- Lipid metabolism and biosynthesis
- Cholesterol and Lipid Metabolism
- Cancer, Lipids, and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Adipose Tissue and Metabolism
- Drug Transport and Resistance Mechanisms
- Alcohol Consumption and Health Effects
- Pancreatic function and diabetes
- Fatty Acid Research and Health
- Metabolomics and Mass Spectrometry Studies
- Diet, Metabolism, and Disease
- Cancer, Hypoxia, and Metabolism
- Tryptophan and brain disorders
- Epigenetics and DNA Methylation
- Plant and animal studies
- Hair Growth and Disorders
- Inflammatory Bowel Disease
- Wound Healing and Treatments
- Insect-Plant Interactions and Control
- Adipokines, Inflammation, and Metabolic Diseases
- Endoplasmic Reticulum Stress and Disease
- Immune Cell Function and Interaction
Cleveland Clinic Lerner College of Medicine
2016-2025
Cleveland Clinic
2016-2025
Case Western Reserve University
2017-2024
University of Miami
2020-2023
University of Illinois Chicago
2023
Cardiff and Vale University Health Board
2022
Case Comprehensive Cancer Center
2017-2021
Cleveland Research (United States)
2021
Grinnell College
1996-2020
University of California, Los Angeles
2018
Macrophage-specific Abca1 knock-out (Abca1(-)(M)(/-)(M)) mice were generated to determine the role of macrophage ABCA1 expression in plasma lipoprotein concentrations and innate immune response macrophages. Plasma lipid chow-fed Abca1(-)(M)(/-)(M) wild-type (WT) indistinguishable. Compared with WT macrophages, macrophages had a >95% reduction protein, failed efflux apoA-I, significant increase free cholesterol (FC) membrane rafts without induction endoplasmic reticulum stress....
We performed silencing and overexpression studies of flavin containing monooxygenase (FMO) 3 in hyperlipidemic mouse models to examine its effects on trimethylamine N-oxide (TMAO) levels atherosclerosis. Knockdown hepatic FMO3 LDL receptor knockout mice using an antisense oligonucleotide resulted decreased circulating TMAO Surprisingly, we also observed significant decreases lipids plasma lipids, ketone bodies, glucose, insulin. transgenic mice, the other hand, increased lipids. Global gene...
Emerging evidence suggests that microbes resident in the human intestine represent a key environmental factor contributing to obesity-associated disorders. Here, we demonstrate gut microbiota-initiated trimethylamine N-oxide (TMAO)-generating pathway is linked obesity and energy metabolism. In multiple clinical cohorts, systemic levels of TMAO were observed strongly associate with type 2 diabetes. addition, circulating associated traits different inbred strains represented Hybrid Mouse...
Glioblastoma (GBM) contains a self-renewing, tumorigenic cancer stem cell (CSC) population which contributes to tumor propagation and therapeutic resistance. While the microenvironment is essential CSC self-renewal, mechanisms by CSCs sense respond microenvironmental conditions are poorly understood. Scavenger receptors broad class of membrane well characterized on immune cells instrumental in sensing apoptotic cellular debris modified lipids. Here, we provide evidence that selectively use...
Glioblastoma ranks among the most aggressive and lethal of all human cancers. Functionally defined glioma stem cells (GSC) contribute to this poor prognosis by driving therapeutic resistance maintaining cellular heterogeneity. To understand molecular processes essential for GSC maintenance tumorigenicity, we interrogated superenhancer landscapes primary glioblastoma specimens in vitro GSCs. GSCs epigenetically upregulated ELOVL2, a key polyunsaturated fatty-acid synthesis enzyme. Targeting...
Large-scale human and mechanistic mouse studies indicate a strong relationship between the microbiome-dependent metabolite trimethylamine N-oxide (TMAO) several cardiometabolic diseases. This study aims to investigate role of TMAO in pathogenesis abdominal aortic aneurysm (AAA) target its parent microbes as potential pharmacological intervention.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructure of surface tungsten oxide species in the trioxide/alumina supported system from x-ray absorption near-edge spectroscopy and Raman spectroscopyJ. A. Horsley, I. E. Wachs, J. M. Brown, G. H. Via, F. D. HardcastleCite this: Phys. Chem. 1987, 91, 15, 4014–4020Publication Date (Print):July 1, 1987Publication History Published online1 May 2002Published inissue 1 July...
Trans-10,cis-12 conjugated linoleic acid (CLA) has previously been shown to be the CLA isomer responsible for CLA-induced reductions in body fat animal models, and we have that this isomer, but not cis-9,trans-11 specifically decreased triglyceride (TG) accumulation primary human adiopcytes vitro. Here investigated mechanism behind isomer-specific, CLA-mediated reduction TG differentiating preadipocytes. insulin-stimulated glucose uptake oxidation, reduced insulin-dependent transporter 4...
The obligate pollination mutualisms between yucca moths and yuccas are some of the most obvious cases coevolution, but phylogenetic origins extent coevolution in these interactions little understood. Ecological information from moth family, Prodoxidae, shows that has evolved at least three times separate behaviors. Passive occurs Greya during nectaring by one species oviposition two other species. Active among prodoxids only once, moths. Several life-history traits necessary for evolution...
Background— Stearoyl-coenzyme A desaturase 1 (SCD1) is a well-known enhancer of the metabolic syndrome. The purpose present study was to investigate role SCD1 in lipoprotein metabolism and atherosclerosis progression. Methods Results— Antisense oligonucleotides were used inhibit mouse model hyperlipidemia (LDLr −/− Apob 100/100 ). In agreement with previous reports, inhibition protected against diet-induced obesity, insulin resistance, hepatic steatosis. Unexpectedly, however, strongly...
Mutations of Comparative Gene Identification-58 (CGI-58) in humans cause triglyceride (TG) accumulation multiple tissues. Mice genetically lacking CGI-58 die shortly after birth due to a skin barrier defect. To study the role integrated lipid and energy metabolism, we utilized antisense oligonucleotides (ASOs) inhibit expression adult mice. Treatment with two distinct CGI-58-targeting ASOs resulted ∼80-95% knockdown protein both liver white adipose tissue. In chow-fed mice, ASO-mediated...
Comparative gene identification 58 (CGI-58) is a lipid droplet-associated protein that promotes the hydrolysis of triglyceride by activating adipose lipase. Loss-of-function mutations in CGI-58 humans lead to Chanarin–Dorfman syndrome, condition which accumulates various tissues, including skin, liver, muscle, and intestines. Therefore, without adequate expression, lipids are stored rather than used for fuel, signaling intermediates, membrane biosynthesis. knockdown mice using antisense...
The epidemiology of bacterial vaginosis (BV) suggests it is sexually transmissible, yet no transmissible agent has been identified. It probable that BV-associated communities are transferred from male to female partners during intercourse; however, the microbiota sexual not well-studied. Pyrosequencing analysis PCR-amplified 16S rDNA was used examine bacteria in monogamous couples with and without BV using vaginal, urethral, penile skin specimens. urethral women significantly more similar...
The serine hydrolase α/β domain 6 (ABHD6) has recently been implicated as a key lipase for the endocannabinoid 2-arachidonylglycerol (2-AG) in brain. However, biochemical and physiological function ABHD6 outside of central nervous system not established. To address this, we utilized targeted antisense oligonucleotides (ASOs) to selectively knock down peripheral tissues order identify vivo substrates understand ABHD6's role energy metabolism. Here, show that selective knockdown metabolic...
Recent studies have identified a genetic variant rs641738 near two genes encoding membrane bound O -acyltransferase domain-containing 7 ( MBOAT7 ) and transmembrane channel-like 4 TMC4 that associate with increased risk of non-alcoholic fatty liver disease (NAFLD), steatohepatitis (NASH), alcohol-related cirrhosis, fibrosis in those infected viral hepatitis (Buch et al., 2015; Mancina 2016; Luukkonen Thabet Viitasalo Krawczyk 2017; 2017). Based on hepatic expression quantitative trait loci...