A5020042218- Metabolomics and Mass Spectrometry Studies
- Effects of Radiation Exposure
- Mitochondrial Function and Pathology
- Radiation Therapy and Dosimetry
- Cancer, Hypoxia, and Metabolism
- Spaceflight effects on biology
- Carcinogens and Genotoxicity Assessment
- Cancer, Lipids, and Metabolism
- Cancer therapeutics and mechanisms
- DNA Repair Mechanisms
- Heat shock proteins research
- Radioactivity and Radon Measurements
- Advanced Chemical Sensor Technologies
- Mass Spectrometry Techniques and Applications
- Molecular Biology Techniques and Applications
- Ultrasound and Hyperthermia Applications
- Radioactive contamination and transfer
- Health, Environment, Cognitive Aging
- Frailty in Older Adults
- Traditional Chinese Medicine Analysis
- Cancer, Stress, Anesthesia, and Immune Response
- Acute Myeloid Leukemia Research
- Cardiac Imaging and Diagnostics
- Medical and Biological Ozone Research
- HIV/AIDS drug development and treatment
Georgetown University Medical Center
2015-2024
Georgetown University
2015-2024
Vince Lombardi Cancer Clinic
2021
U-M Rogel Cancer Center
2021
Anna Needs Neuroblastoma Answers
2019
Washington Center
2016
Georgetown Lombardi Comprehensive Cancer Center
2010
University of Maryland, Baltimore
2006-2008
Beltsville Agricultural Research Center
2005
Abstract Heightened threats for nuclear terrorism using improvised devices (IND) necessitate the development of biodosimetry assays that could rapidly assess thousands individuals. However, radiation exposures from an IND may be complex due to mixed fields neutrons and photons (γ-rays), shielding buildings, proximity epicenter among others. In this study we utilized lipidomics analyze serum samples mice exposed various percentages X-rays a total dose 3 Gy. Triacylglycerides,...
Background Pneumonia remains the leading cause of death in young children globally and improved diagnostics are needed to better identify cases reduce case fatality. Metabolomics, a rapidly evolving field aimed at characterizing metabolites biofluids, has potential improve range diseases. The objective this pilot study is apply metabolomic analysis childhood pneumonia explore its diagnosis high-burden setting. Methodology/Principal Findings Eleven with World Health Organization (WHO)-defined...
Metabolomics, the global study of small molecules in a particular system, has past few years risen to become primary -omics platform for metabolic processes. With ever-increasing pool quantitative data yielded from metabolomic research, specialized methods and tools with which analyze extract meaningful conclusions these are becoming more crucial. Furthermore, depth knowledge expertise required undertake metabolomics oriented is daunting obstacle investigators new field. As such, we have...
The emergence of the threat radiological terrorism and other incidents has led to need for development fast, accurate noninvasive methods detection radiation exposure. purpose this study was extend metabolomic biomarker discovery humans, as previous studies have focused on mice. Urine collected from patients undergoing total body irradiation at Memorial Sloan-Kettering Cancer Center prior hematopoietic stem cell transplantation 4–6 h postirradiation (a single dose 1.25 Gy) 24 (three...
Exposure to ionizing radiation has dramatically increased in modern society, raising serious health concerns. The molecular response radiation, however, is still not completely understood. Here, we screened mouse serum for metabolic alterations following an acute exposure γ using a multiplatform mass-spectrometry-based strategy. A global, profiling revealed that undergoes series of significant exposure. We identified and quantified bioactive metabolites belonging key biochemical pathways...
Human space exploration poses inherent risks to astronauts' health, leading molecular changes that can significantly impact their well-being. These alterations encompass genomic instability, mitochondrial dysfunction, increased inflammation, homeostatic dysregulation, and various epigenomic changes. Remarkably, these bear similarities those observed during the aging process on Earth. However, our understanding of connection between shifts disease development in remains limited. Frailty...
Due to concerns surrounding potential large-scale radiological events, there is a need determine robust radiation signatures for the rapid identification of exposed individuals, which can then be used guide development compact field deployable instruments assess individual dose. Metabolomics provides technology process easily accessible biofluids and rigorous quantitative biomarkers with mass spectrometry (MS) platforms. While multiple studies have utilized murine models biomarkers, limited...
Abstract Concern over potential exposures of ionizing radiation (IR) to large populations has emphasized the need for rapid and reliable methods biodosimetry determine absorbed dose required triage. Lipidomics emerged as a powerful technique large-scale lipid identification quantification. Indirect effects from IR exposure generate reactive oxygen species (ROS) through water hydrolysis may subsequently damage cellular lipids. Thus, specific affected molecules represents possible targets...
Metabolomics on easily accessible biofluids has the potential to provide rapid identification and distinction between stressors inflammatory states. In event of a radiological event, individuals with underlying medical conditions could present similar symptoms radiation poisoning, prominently nausea, diarrhea, vomiting fever. exposure in mice provided valuable biomarkers, this study we aimed identify biomarkers lipopolysaccharide (LPS) compare contrast ionizing radiation. LPS treatment leads...
There is a need for research to rapidly determine an individual's absorbed dose and its potential health effects after radiological or nuclear event that could expose large portions of population ionizing radiation (IR). Studies on biomarker identification exposure aid in biodosimetry, identifying individual absorbed, as well biologic response, administering immediate proper medical care. Metabolomics easily accessible biofluids emerging field with high-throughput biodosimetry. While...
Abstract The search for and development of radiation countermeasures to treat acute lethal injury has been underway the past six decades, resulting in identification multiple classes countermeasures. However, date only granulocyte colony-stimulating factor (Neupogen) PEGylated (Neulasta) have approved by U.S. Food Drug Administration treatment hematopoietic syndrome. Gamma-tocotrienol demonstrated radioprotective efficacy murine nonhuman primate models. Currently, this agent is under...
Ionizing radiation (IR) directly damages cells and tissues or indirectly them through reactive free radicals that may lead to longer term adverse sequelae such as cancers, persistent inflammation, possible death. Potential exposures include nuclear reactor accidents, improper disposal of equipment containing radioactive materials medical errors, terrorist attacks. Metabolomics (comprehensive analysis compounds <1 kDa) by mass spectrometry (MS) has been proposed a tool for high-throughput...
The potential for radiological accidents and nuclear terrorism has increased the need development of new rapid biodosimetry methods. In addition, in a clinical setting issue an individual's radiosensitivity should be taken into consideration during radiotherapy. We utilized metabolomics lipidomics to investigate changes metabolites serum samples following exposure total body ionizing radiation humans. Serum was collected prior irradiation, at 3–8 h after single dose 1.25–2 Gy, 24 with...
Rapid assessment of radiation signatures in noninvasive biofluids may aid assigning proper medical treatments for acute syndrome (ARS) and delegating limited resources after a nuclear disaster. Metabolomic platforms allow rapid screening biofluid show promise differentiating quality time postexposure. Here, we use global metabolomics to differentiate temporal effects (1-60 d) found nonhuman primate (NHP) urine serum small molecule 4 Gy total body irradiation. Random Forests analysis...
Pancreatic ductal adenocarcinoma (PDAC) is the fourth leading cause of cancer deaths worldwide with less than a 6% 5-year survival rate. PDAC associated poor prognosis based on late stage diagnosis disease. Current diagnostic tests lack sensitivity and specificity to identify markers early staging. Metabolomics has provided biomarkers for various diseases, stressors, environmental exposures. In this study we utilized p48-Cre/LSL-KrasG12D mouse model age-matched wild type mice. This shows...
Threats of nuclear terrorism coupled with potential unintentional ionizing radiation exposures have necessitated the need for large-scale response efforts such events, including high-throughput biodosimetry medical triage. Global metabolomics utilizing mass spectrometry (MS) platforms has proven an ideal tool generating large compound databases relative quantification and structural information in a short amount time. Determining metabolite panels requires experimentation to evaluate many...
High-throughput methods to assess radiation exposure are a priority due concerns that include nuclear power accidents, the spread of weapon capability, and risk terrorist attacks. Metabolomics, assessment small molecules in an easily accessible sample, is most recent method be applied for identification biomarkers biological response with useful dose-response profile. Profiling biomarker frequently done using LC-MS platform which has limited throughput time-consuming nature chromatography....
Radiation exposure is an important public health issue due to a range of accidental and intentional threats. Prompt effective large-scale screening appropriate use medical countermeasures (MCM) mitigate radiation injury requires rapid methods for determining the dose. In number studies, metabolomics has identified small-molecule biomarkers responding Differential mobility spectrometry-mass spectrometry (DMS-MS) been used similar compounds high-throughput detection quantitation. this study,...