// Benjamin J. Chen 1 , Ravi Dashnamoorthy 2 Pallavi Galera Vladislav Makarenko Hong Chang 3 Srimoyee Ghosh 4 and Andrew M. Evens Department of Pathology, University Massachusetts Medical School, Worcester, MA, USA Division Blood Disorders, Rutgers Cancer Institute New Jersey, Brunswick, NJ, for Clinical Research Health Policy Studies the Biostatistics, Epidemiology, Design (BERD) Center, Tufts Boston, Tesaro, Waltham, Correspondence to: Chen, email: Benjamin.chen@umassmemorial.org Keywords:...

<br><b>Context:</b> Brain tumor is one of the most devastating forms human illness, especially when occurring in posterior fossa and involving brainstem. Tumors are considered some critical brain lesions. This primarily due to limited space within fossa, as well potential involvement vital brainstem nuclei. <b>Aims:</b> The aim this study analyze incidence, clinical features, surgical outcome, complications, prognosis a series 37 pediatric patients with tumors who underwent surgery between...

Natural killer (NK) cells are phenotypically and functionally diverse lymphocytes that recognize kill cancer cells. The susceptibility of target to NK cell-mediated cytotoxicity depends on the strength balance regulatory (activating/inhibitory) ligands expressed cell surface. We performed gene expression arrays determine patterns associated with B-cell non-Hodgkin lymphoma (b-NHL). Microarray analyses revealed significant upregulation a multitude NK-activating costimulatory across varied...

Apoptosis is a major mechanism of cancer cell destruction by chemotherapy and radiotherapy. The anthracycline class antitumor drugs undergoes redox cycling in living cells producing increased amounts reactive oxygen species semiquinone radical, both which can cause DNA damage, consequently trigger apoptotic death cells. We show here that MCF-7 overexpressing thioredoxin (Trx) were more response to daunomycin. Trx overexpression the generation superoxide anion (O2˙-) anthracycline-treated...

A genomic catalogue of protein-protein interactions is a rich source information, particularly for exploring the relationships between proteins. Numerous systems-wide and small-scale experiments have been conducted to identify interactions; however, our knowledge all any one species incomplete, alternative means expand these network maps needed. We therefore took comparative biology approach predict across five (human, mouse, fly, worm, yeast) developed InterologFinder research biologists...

Proteasome-regulated NF-κB has been shown to be important for cell survival in T-cell lymphoma and Hodgkin models. Several new small-molecule proteasome inhibitors are under various stages of active preclinical clinical development. We completed a comprehensive examination the efficacy associated biologic effects second-generation inhibitor, ixazomib, cells vivo SCID mouse demonstrated that ixazomib induced potent death all lines at clinically achievable concentrations. In addition, it...

Abstract There remains a need to identify new sensitive diagnostic and predictive blood-based platforms in lymphoma. We previously discovered novel circulating microRNA (miRNA) signature Smurf2-deficient mouse model that spontaneously develops diffuse large B-cell lymphoma (DLBCL). Herein, we investigated this 10-miRNA (miR-15a, let-7c, let-7b, miR-27a, miR-10b, miR-18a, miR-497, miR-130a, miR24, miR-155) human cell lines, mice engrafted with patient-derived xenografts (PDXs), DLBCL patient...

Damage initiates a pleiotropic cellular response aimed at survival when appropriate. To identify genes required for damage survival, we used cell-based RNAi screen against the Drosophila genome and alkylating agent methyl methanesulphonate (MMS). Similar studies performed in other model organisms report that may involve processes than central DNA repair components, yet an intuitive systems level view of components their interrelationship, contextual importance has been lacking. Further, by...

Idiopathic pulmonary fibrosis (IPF) is a chronic lung disease that leads to respiratory decline due scarring and thickening of tissues. Multiple pathways contribute the fibrotic process in this disease, such as inflammation, epithelial mesenchymal transition oxidative stress. The RhoA/ROCK signaling pathway key regulator profibrotic signaling, it affects organization actin-myosin remodeling extracellular matrix. ROCK1/2, downstream effector RhoA, overexpressed IPF patients promising target...

Hyperoxia has been shown to cause DNA damage resulting in growth arrest of cells p53-dependent, as well p53-independent, pathways. Although H 2 O and other peroxides have induce ataxia telangiectasia-mutated (ATM)-dependent p53 phosphorylation response damage, the signal transduction mechanisms hyperoxia are currently unknown. Here we demonstrate that phosphorylates Ser15 residue independently ATM. phosphorylated (Ser15) DNA-dependent protein kinase null (DNA-PK -/- ) cells, indicating it...

Abstract Purpose: Darinaparsin (Zio-101) is a novel organic arsenical compound with encouraging clinical activity in relapsed/refractory T-cell lymphoma (TCL) and Hodgkin (HL); however, little known about its mechanism of action. Experimental Design: TCL cell lines (Jurkat, Hut78, HH) HL (L428, L540, L1236) were examined for vitro death by MTT assay Annexin V–based flow cytometry. Jurkat L540-derived xenografts SCID mice vivo tumor inhibition survival. Biologic effects darinaparsin on the...

Genome-wide RNA interference (RNAi) screening allows investigation of the role individual genes in a process choice. Most RNAi screens identify large number with continuous gradient assessed phenotype. Screeners must decide whether to examine most robust phenotype or full that cause an effect and how candidate genes. The authors have used Drosophila cells viability 384-well plate format compare 2 screens, untreated control treatment. They multiple normalization methods, which take advantage...

// J. Tyson McDonald 1, * , Athena Kritharis 2, Afshin Beheshti 3 Monika Pilichowska 4 Kristine Burgess 5 Luisel Ricks-Santi 1 Elizabeth McNiel Cheryl A. London Dashnamoorthy Ravi 2 and Andrew M. Evens Cancer Research Center, Hampton University, Hampton, VA, USA Division of Blood Disorders, Rutgers Institute New Jersey, Brunswick, NJ, WYLE, NASA Ames Moffett Field, CA, Pathology, Tufts Medical Boston, MA, Cummings School Veterinary Medicine, These authors contributed equally to this work...

For over two decades, Rituximab and CHOP combination treatment (rCHOP) has remained the standard approach for diffuse large B-cell lymphoma (DLBCL). Despite numerous clinical trials exploring alternatives, few options have shown any promise at further improving patient survival recovery rates. A wave of new therapeutic approaches recently been in development with rise immunotherapy cancer, however, cost is prohibitive testing all promising approaches. Improved methods early drug screening...

The importance of programmed cell death or apoptosis in the maintenance tissue homoeostasis and pathogenesis oral cancer was analysed relation to regulatory proteins, proliferation tumour histology.The extent defined by morphological criteria TUNEL (terminal deoxy nucleotidyl transferase-mediated dUTP biotin nick end labelling) assay. p53, bax, bcl-2 cyclin D1 expression evaluated immunocytochemistry. presence mutant p53 using a p53-specific ELISA. An inverse correlation observed between...

Bronchopulmonary dysplasia (BPD) is a major complication of premature infants who receive prolonged ventilatory support. The pathophysiology BPD involves oxidant injury, baro/volutrauma, and disordered lung repair. Exposure that poorly adapted for air breathing (>3% oxygen in fetal lung) to higher concentration can cause significant injury. Cell growth differentiation the developing require selective ordered cell division. As hyperoxia increase expression cell-cycle checkpoints arrest cells,...

Background In vitro cell culture experiments with primary cells have reported that proliferation is retarded in the presence of ambient compared to physiological O2 levels. Cancer primarily a disease aberrant proliferation, therefore, studying cancer grown under may be undesirable. To understand better impact on propagation vitro, we growth potential panel ovarian lines (21%) or (3%) O2. Principal Findings Our observations demonstrate similar cells, many maintain an inherent sensitivity O2,...

One method to understand and evaluate an experiment that produces a large set of genes, such as gene expression microarray analysis, is identify overrepresentation or enrichment for biological pathways. Because pathways are able functionally describe the much effort has been made collect curated into publicly accessible databases. When combining disparate databases, highly related redundant exist, making their consolidation pathway concepts essential. This will facilitate unbiased,...