A5022538301- Innovative Microfluidic and Catalytic Techniques Innovation
- 3D Printing in Biomedical Research
- Microfluidic and Capillary Electrophoresis Applications
- Advanced Biosensing Techniques and Applications
- CAR-T cell therapy research
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Bio-sensing Technologies
- Immune Cell Function and Interaction
- Lymphoma Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Bacterial Identification and Susceptibility Testing
- Single-cell and spatial transcriptomics
- Force Microscopy Techniques and Applications
- Cellular Mechanics and Interactions
- Sports injuries and prevention
- CRISPR and Genetic Engineering
- Nanoparticle-Based Drug Delivery
- RNA Interference and Gene Delivery
- Electrowetting and Microfluidic Technologies
- Virus-based gene therapy research
Northeastern University
2014-2018
Universidad del Noreste
2015
Here we describe a robust, microfluidic technique to generate and analyze 3D tumor spheroids, which resembles microenvironment can be used as more effective preclinical drug testing screening model. Monodisperse cell-laden alginate droplets were generated in polydimethylsiloxane (PDMS) devices that combine T-junction droplet generation external gelation for spheroid formation. The proposed approach has the capability incorporate multiple cell types. For purposes of our study, spheroids with...
Cell-cell communication mediates immune responses to physiological stimuli at local and systemic levels. Intercellular occurs via a direct contact between cells as well by secretory contact-independent mechanisms. However, there are few existing methods that allow quantitative resolution of contact-dependent independent cellular processes in rapid, precisely controlled, dynamic format. This study utilizes high-throughput microfluidic droplet array platform analyze cell-cell interaction...
We developed a droplet microfluidics-based phenotypic drug screening platform for analysis of single cell responses to cancer therapeutics.
Natural killer (NK) cells are phenotypically and functionally diverse lymphocytes that recognize kill cancer cells. The susceptibility of target to NK cell-mediated cytotoxicity depends on the strength balance regulatory (activating/inhibitory) ligands expressed cell surface. We performed gene expression arrays determine patterns associated with B-cell non-Hodgkin lymphoma (b-NHL). Microarray analyses revealed significant upregulation a multitude NK-activating costimulatory across varied...
Characterization of the heterogeneity in immune reactions requires assessing dynamic single cell responses as well interactions between various subsets. Maturation and activation effector cells is regulated by contact-dependent soluble factor-mediated paracrine signalling. Currently there are few methods available that allow investigation both processes simultaneously without physically constraining non-adherent eliminating crosstalk from neighboring pairs. We describe here a microfluidic...
Microfluidic droplets are used to isolate cell pairs and prevent crosstalk with neighboring cells, while permitting free motility interaction within the confined space. Dynamic analysis of cellular heterogeneity in has provided insights various biological processes. Droplet manipulation methods such as fusion fission make it possible precisely regulate localized environment a droplet deliver reagents required. strategies achieved by passive mechanisms preserve viability easier fabricate...