A5003932077- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Clostridium difficile and Clostridium perfringens research
- Streptococcal Infections and Treatments
- Inflammasome and immune disorders
- Antimicrobial Peptides and Activities
- Bacterial Identification and Susceptibility Testing
- Bacterial Genetics and Biotechnology
- Metabolomics and Mass Spectrometry Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Microbial Metabolism and Applications
- Medical and Biological Ozone Research
- Immune Response and Inflammation
- Nitric Oxide and Endothelin Effects
- Gut microbiota and health
- PARP inhibition in cancer therapy
- Calcium signaling and nucleotide metabolism
- Lipid Membrane Structure and Behavior
University of North Carolina at Chapel Hill
2019-2023
Staphylococcus aureus is a leading human pathogen that frequently causes chronic and relapsing infections. Antibiotic-tolerant persister cells contribute to frequent antibiotic failure in patients. Macrophages represent an important niche during S. bacteremia, recent work has identified role for oxidative burst the formation of antibiotic-tolerant aureus. We find host-derived peroxynitrite, reaction product superoxide nitric oxide, main mediator tolerance macrophages. Using collection...
Antibiotic tolerance and antibiotic resistance are the two major obstacles to efficient reliable treatment of bacterial infections. Identifying adjuvants that sensitize resistant tolerant bacteria killing may lead development superior treatments with improved outcomes. Vancomycin, a lipid II inhibitor, is frontline for treating methicillin-resistant Staphylococcus aureus other Gram-positive However, vancomycin use has led increasing prevalence strains reduced susceptibility vancomycin. Here,...
Bacterial biofilms, often associated with chronic infections, respond poorly to antibiotic therapy and frequently require surgical intervention. Biofilms harbor persister cells, metabolically indolent which are tolerant most conventional antibiotics. In addition, the biofilm matrix can act as a physical barrier, impeding diffusion of Novel therapeutic approaches improve killing, but usually fail achieve eradication. Failure eradicate leads relapsing infection, is major financial healthcare...
is a leading human pathogen that frequently causes relapsing infections. The failure of antibiotics to eradicate infection contributes relapse. Host-pathogen interactions have substantial impact on antibiotic susceptibility and the formation tolerant cells. In this study, we interrogate how major
Interactions between Staphylococcus aureus and the host immune system can have significant impacts on antibiotic efficacy, suggesting that targeting modulating response to S. infection may improve efficacy outcome. As we've previously shown, high levels of reactive oxygen species (ROS), associated with an M1-like proinflammatory macrophage response, potently induce tolerance in aureus. Although is critical for initial control pathogen burden, recent studies demonstrate modulation...
ABSTRACT Staphylococcus aureus is a leading human pathogen that frequently causes chronic and relapsing infections. Antibiotic tolerant persister cells contribute to frequent antibiotic failure in patients. Macrophages represent an important niche during S. bacteremia recent work has identified role for oxidative burst the formation of . We find host-derived peroxynitrite, reaction product superoxide nitric oxide, main mediator tolerance macrophages. Using collection clinical isolates, we...
Abstract Staphylococcus aureus is a leading human pathogen that frequently causes relapsing infections. Host-pathogen interactions have been shown to substantial impacts on antibiotic susceptibility and the formation of tolerant cells. In this study, we interrogate how major S. virulence factor, α-toxin, interacts with macrophages alter microenvironment pathogen, thereby influencing its antibiotics. We find α-toxin-mediated activation NLRP3 inflammasome induces tolerance in host cell...
Staphylococcus Aureus is a leading human pathogen that frequently causes relapsing infections. Host-pathogen interactions have been shown to substantial impacts on antibiotic susceptibility and the formation of tolerant cells. In this study, we interrogate how major S. aureus virulence factor, α-toxin, interacts with macrophages alter microenvironment pathogen, thereby influencing its antibiotics. We find α-toxin-mediated activation NLRP3 inflammasome induces tolerance in host cell...
Abstract Antibiotic tolerance and antibiotic resistance are the two major obstacles to efficient reliable treatment of bacterial infections. Identifying adjuvants that sensitize resistant tolerant bacteria killing may lead development superior treatments with improved outcomes. Vancomycin, a lipid II inhibitor, is clinical importance for Gram-positive Here we show unsaturated fatty acids (UFAs) vancomycin act synergistically rapidly kill S. aureus , including populations. Our results suggest...