A5071371014- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Clostridium difficile and Clostridium perfringens research
- Wound Healing and Treatments
- Mycobacterium research and diagnosis
- Bacterial Identification and Susceptibility Testing
- Tuberculosis Research and Epidemiology
- Antimicrobial Peptides and Activities
- Metabolomics and Mass Spectrometry Studies
- Bacterial Genetics and Biotechnology
- Bacillus and Francisella bacterial research
- Escherichia coli research studies
- Dermatologic Treatments and Research
- Vibrio bacteria research studies
- Linguistics and language evolution
- Bacteriophages and microbial interactions
- Ultrasound and Hyperthermia Applications
- Medieval Literature and History
- Lipid Membrane Structure and Behavior
- Byzantine Studies and History
- Microbial Metabolism and Applications
University of North Carolina at Chapel Hill
2020-2024
University of Central Florida
2018
Gram-negative bacterial bloodstream infections (GNB-BSI) are common and frequently lethal. Despite appropriate antibiotic treatment, relapse of GNB-BSI with the same strain is associated poor clinical outcomes high healthcare costs. The role persister cells, which sub-populations bacteria that survive for prolonged periods in presence bactericidal antibiotics, unclear. Using a cohort patients relapsed GNB-BSI, we aimed to determine how pathogen evolves within patient between initial...
Antibiotic tolerance and antibiotic resistance are the two major obstacles to efficient reliable treatment of bacterial infections. Identifying adjuvants that sensitize resistant tolerant bacteria killing may lead development superior treatments with improved outcomes. Vancomycin, a lipid II inhibitor, is frontline for treating methicillin-resistant Staphylococcus aureus other Gram-positive However, vancomycin use has led increasing prevalence strains reduced susceptibility vancomycin. Here,...
Bacterial biofilms, often associated with chronic infections, respond poorly to antibiotic therapy and frequently require surgical intervention. Biofilms harbor persister cells, metabolically indolent which are tolerant most conventional antibiotics. In addition, the biofilm matrix can act as a physical barrier, impeding diffusion of Novel therapeutic approaches improve killing, but usually fail achieve eradication. Failure eradicate leads relapsing infection, is major financial healthcare...
Chronic wounds are frequently infected with bacterial biofilms which pose a barrier to drug diffusion, uptake, and facilitate tolerance, thereby prolonging the healing process in chronic increasing likelihood of relapse. A major contributor relapse is persister cells, subset resilient high antibiotic doses. Previous work by our group showcased significantly improved gentamicin efficacy diabetic murine wound model using ultrasound, nanodroplets, an anti-persister drug. Here, we aim optimize...
Nontuberculous mycobacteria (NTM) species are a rising threat, especially to patients living with pulmonary comorbidities. Current point-of-care diagnostics fail adequately identify and differentiate NTM from Mycobacterium tuberculosis (Mtb). Definitive culture- molecular-based testing can take weeks months requires sending samples out specialized diagnostic laboratories.In this proof-of-concept study, we developed an assay based on PCR amplification of 16S ribosomal RNA (rRNA) rrs genes by...
ABSTRACT Pseudomonas aeruginosa frequently causes chronic lung infection in individuals with muco-obstructive airway diseases (MADs). Chronic P. infections are difficult to treat, primarily owing antibiotic treatment failure, which is often observed the absence of antimicrobial resistance. In MADs, forms biofilm-like aggregates within luminal mucus. While contribution mucin hyperconcentration towards tolerance has been described, mechanism for driven and influence have not fully elucidated....
The rise in antibiotic resistance limits the availability of antibiotics to treat bacterial infections. Despite this, development pipelines remain sparse which makes using adjuvants reverse a promising therapeutic strategy. use vancomycin, frontline used hospitalized patients with methicillin-resistant
Bacterial biofilms, often associated with chronic infections, respond poorly to antibiotic therapy and frequently require surgical intervention. Biofilms harbor persister cells, metabolically indolent which are tolerant most conventional antibiotics. In addition, the biofilm matrix can act as a physical barrier, impeding diffusion of Novel therapeutic approaches improve killing, but usually fail achieve eradication. Failure eradicate leads relapsing infection, major financial healthcare...
Abstract Background Gram-negative bacterial bloodstream infections (GNB-BSI) are common and frequently lethal. Many patients experience recurrent GNB-BSI for unclear reasons. This study explores how antibiotic tolerance may lead to infection relapse.Figure 1:Flow diagram of relapsed versus infection. Approximately 63% due relapse (almost genetically identical isolate) 37% reinfection (genetically distinct isolate).Figure 2:Relapsed isolate exhibits increased in murine bacteremia model....
Abstract Antibiotic tolerance and antibiotic resistance are the two major obstacles to efficient reliable treatment of bacterial infections. Identifying adjuvants that sensitize resistant tolerant bacteria killing may lead development superior treatments with improved outcomes. Vancomycin, a lipid II inhibitor, is clinical importance for Gram-positive Here we show unsaturated fatty acids (UFAs) vancomycin act synergistically rapidly kill S. aureus , including populations. Our results suggest...
Bacterial biofilms are the leading cause of delayed healing in chronic wounds. Aminoglycoside antibiotics, such as gentamicin (Gent), ineffective against biofilm cells they maintain proton motive force below threshold for drug uptake. We employ a novel aminoglycoside adjuvant, palmitoleic acid (PA), to facilitate Here, we propose dual strategy eradicate wound infection; utilizing ultrasound-stimulated nanodroplets (US+ND) improve penetration combination Gent/PA. A infection model was...