A5013630399- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Antibiotic Resistance in Bacteria
- Microbial Natural Products and Biosynthesis
- Computational Drug Discovery Methods
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Biochemical and Molecular Research
- Diagnosis and treatment of tuberculosis
- RNA and protein synthesis mechanisms
- Cancer therapeutics and mechanisms
- Bacterial Infections and Vaccines
- Biosensors and Analytical Detection
- Marine Sponges and Natural Products
- Pharmacological Effects of Natural Compounds
- Hemoglobin structure and function
- Pharmacogenetics and Drug Metabolism
- Pneumocystis jirovecii pneumonia detection and treatment
- Lipid Membrane Structure and Behavior
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Antimicrobial Peptides and Activities
- Bacteriophages and microbial interactions
- Pneumonia and Respiratory Infections
- Immune cells in cancer
University of Central Florida
2016-2025
University of Florida
2025
Franklin College
2025
University of Georgia
2025
Orlando Health
2017
Florida College
2016
Cornell University
2005-2014
University of Geneva
2009
New York State College of Veterinary Medicine
2009
University of Oklahoma Health Sciences Center
1996-2004
Intracellular pathogens such as Mycobacterium tuberculosis have evolved strategies for coping with the pressures encountered inside host cells. The ability to coordinate global gene expression in response environmental and internal cues is one key their success. Prolonged survival replication within macrophages, a virulence trait of M. tuberculosis, requires dynamic adaptation diverse changing conditions its phagosomal niche. However, physiological adaptations during different phases this...
Tuberculosis exerts a tremendous burden on global health, with ∼9 million new infections and ∼2 deaths annually. The Mycobacterium tuberculosis complex (MTC) was initially regarded as highly homogeneous population; however, recent data suggest the causative agents of are more genetically functionally diverse than appreciated previously. impact this natural variation virulence clinical manifestations pathogen remains largely unknown. This report examines effect genetic diversity among MTC...
Successful chemotherapy against Mycobacterium tuberculosis (Mtb) must eradicate the bacterium within context of its host cell. However, our understanding impact this environment on antimycobacterial drug action remains incomplete. Intriguingly, we find that Mtb in myeloid cells isolated from lungs experimentally infected mice exhibit tolerance to both isoniazid and rifampin a degree proportional activation status cells. These data are confirmed by vitro infections resting versus activated...
To generate efficient vaccines and cures for Mycobacterium tuberculosis, we need a far better understanding of its modes infection, persistence, spreading. Host cell entry the establishment replication niche are well understood, but little is known about how tubercular mycobacteria exit host cells disseminate infection. Using social amoeba Dictyostelium as genetically tractable pathogenic mycobacteria, discovered that M. tuberculosis marinum, not avium, ejected from through an actin-based...
Following phagocytosis by macrophages, Mycobacterium tuberculosis (Mtb) senses the intracellular environment and remodels its gene expression for growth in phagosome. We have identified an acid phagosome regulated (aprABC) locus that is unique to Mtb complex whose induced during acidic environments vitro macrophages. Using aprA promoter, we generated a strain exhibits high levels of inducible fluorescence response medium aprABC dependent on two-component regulator phoPR, linking phoPR...
ABSTRACT The dormant phenotype acquired by Mycobacterium tuberculosis during infection poses a major challenge in disease treatment, since these bacilli show tolerance to front-line drugs. Therefore, it is imperative find novel compounds that effectively kill bacteria. By screening 4,400 marine natural product samples against dual-fluorescent M. under both replicating and nonreplicating conditions, we have identified are selectively active . This validates our strategy of all assays as...
ABSTRACT Bacterial topoisomerase functions are required for regulation of DNA supercoiling and overcoming the topological barriers that encountered during many vital cellular processes. gyrase IV type IIA bacterial family important clinical targets antibacterial therapy. Topoisomerase I, belonging to IA family, has recently been validated as a potential antitubercular target. The I activity shown be essential viability infection in murine model tuberculosis. Mixture-based combinatorial...
Bacterial biofilms are surface-attached communities consisting of non-replicating persister cells encased within an extracellular matrix biomolecules. Unlike bacteria that have acquired resistance to antibiotics, enable demonstrate innate tolerance toward all classes conventional antibiotic therapies. It is estimated 50-80% bacterial infections biofilm associated, which considered the underlying cause chronic and recurring infections. Herein, we report a modular three-step synthetic route...
ABSTRACT Trehalose 6,6′-dimycolate (TDM) is a cell wall glycolipid and an important virulence factor of mycobacteria. In order to study the role TDM in innate immune response Mycobacterium tuberculosis , microarray analysis was used examine gene regulation murine bone marrow-derived macrophages 90-μm-diameter polystyrene microspheres coated with TDM. A large number genes, particularly those involved macrophage function, were up- or downregulated these TDM-coated beads compared control beads....
In the blink of eye: A cascade two deoxyribozymes was designed for rapid visual detection bacterial 16S rRNA. The limit is 12.5 ng by naked eye, with ability to differentiate between closely related pathogenic and nonpathogenic species. As a service our authors readers, this journal provides supporting information supplied authors. Such materials are peer reviewed may be re-organized online delivery, but not copy-edited or typeset. Technical support issues arising from (other than missing...
The DNA machine unwinds RNA; selectively recognizes targeted fragment; attracts fluorogenic substrate and catalytically multiplies fluorescent signal.
Signals modulating the production of Mycobacterium tuberculosis (Mtb) virulence factors essential for establishing long-term persistent infection are unknown. The WhiB3 redox regulator is known to regulate Mtb factors, however mechanisms this modulation To advance our understanding involved in regulation, we performed vitro, intraphagosomal and infected host expression analyses. Our analyses conjunction with extracellular flux demonstrated that maintains bioenergetic homeostasis response...
Pathogenic bacteria demonstrate incredible abilities to evade conventional antibiotics through the development of resistance and formation dormant, surface-attached biofilms. Therefore, agents that target eradicate planktonic biofilm are significant interest. We explored a new series halogenated phenazines (HP) use N-aryl-2-nitrosoaniline synthetic intermediates enabled functionalization 3-position this scaffold. Several HPs demonstrated potent antibacterial biofilm-killing activities (e.g.,...
A deep-water sponge of the genus Spongosorites has yielded a bis-indole alkaloid which we have named dragmacidin G. Dragmacidin G was first reported by us in patent literature and recently been Hitora et al. from Lipastrotheya. is this series compounds to pyrazine ring linking two indole rings. It also rare N-(2-mercaptoethyl)-guanidine side chain. shows broad spectrum biological activity including inhibition methicillin-resistant Staphylococcus aureus, Mycobacterium tuberculosis, Plasmodium...
Mycobacterium abscessus ( Mab ), an emerging opportunistic pathogen, predominantly infects individuals with underlying pulmonary diseases such as cystic fibrosis (CF). Current treatment outcomes for infections are poor due to Mab’s inherent antibiotic resistance and unique host interactions that promote phenotypic tolerance hinder drug access. The hypoxic, mucus-laden airways in the CF lung antimicrobial phagosome within macrophages represent hostile niches must overcome via alterations gene...