A5085866405- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Antibiotic Use and Resistance
- Antimicrobial Resistance in Staphylococcus
- Infective Endocarditis Diagnosis and Management
- Streptococcal Infections and Treatments
- Urinary Tract Infections Management
- Escherichia coli research studies
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Clostridium difficile and Clostridium perfringens research
- Enterobacteriaceae and Cronobacter Research
- Microbial Metabolism and Applications
- Immune Response and Inflammation
- Orthopedic Infections and Treatments
- Sepsis Diagnosis and Treatment
- Neonatal and Maternal Infections
- Bacterial Genetics and Biotechnology
- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Gut microbiota and health
- Cystic Fibrosis Research Advances
- Transplantation: Methods and Outcomes
Duke University
2016-2025
Texas A&M University
2023
Duke Medical Center
2013-2022
Duke University Hospital
2021
University of California, Los Angeles
2021
Duke University Health System
2020
Office of Infectious Diseases
2015-2018
Clinical Research Institute
2014-2015
Med Center
2015
Boston University
2007-2010
Machine learning approaches offer the potential to systematically identify transcriptional regulatory interactions from a compendium of microarray expression profiles. However, experimental validation performance these methods at genome scale has remained elusive. Here we assess global four existing classes inference algorithms using 445 Escherichia coli Affymetrix arrays and 3,216 known E. RegulonDB. We also developed applied context likelihood relatedness (CLR) algorithm, novel extension...
The impact of bacterial species on outcome in bloodstream infections (BSI) is incompletely understood. We evaluated the BSI mortality, with adjustment for patient, bacterial, and treatment factors. From 2002 to 2015, all adult inpatients monomicrobial caused by Staphylococcus aureus or Gram-negative bacteria at Duke University Medical Center were prospectively enrolled. Kaplan-Meier curves multivariable Cox regression propensity score models used examine species-specific mortality. Of 2,659...
OBJECTIVE To describe the epidemiology of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (ESBL-EC) and Klebsiella pneumoniae (ESBL-KP) infections DESIGN Retrospective cohort SETTING Inpatient care at community hospitals PATIENTS All patients with ESBL-EC or ESBL-KP METHODS from 26 were prospectively entered into a centralized database January 2009 to December 2014. RESULTS A total 925 caused by (10.5 per 100,000 patient days) 463 (5.3 identified during 8,791,243 days...
Klebsiella pneumoniae is a common cause of sepsis and particularly associated with healthcare-associated infections. New strategies are needed to prevent or treat infections due the emergence multi-drug resistant K. pneumoniae. The goal this study was determine global diversity distribution O (lipopolysaccharide) K (capsular polysaccharide) antigens on large (>500) collection strains isolated from blood inform vaccine development efforts. A total 645 isolates were collected patients...
The clinical and economic impacts of bloodstream infections (BSI) due to multidrug-resistant (MDR) Gram-negative bacteria are incompletely understood. From 2009 2015, all adult inpatients with BSI at our institution were prospectively enrolled. MDR status was defined as resistance ≥3 antibiotic classes. Clinical outcomes inpatient costs associated the phenotype identified. Among 891 unique patients BSI, 292 (33%) infected bacteria. In an adjusted analysis, only history infection versus...
Abstract Background We conducted a longitudinal study to evaluate changes in the clinical presentation and epidemiology of Staphylococcus aureus bacteremia (SAB) an academic, US medical center. Methods Consecutive patients with monomicrobial SAB were enrolled from January 1995 December 2015. Each person’s initial bloodstream S. isolate was genotyped using spa typing. Clonal complexes (CCs) assigned Ridom StaphType software. Changes over time both patient bacterial characteristics estimated...
Abstract Background Pseudomonas aeruginosa is an opportunistic pathogen that causes a wide range of acute and chronic infections frequently associated with healthcare-associated infections. Because its ability to rapidly acquire resistance antibiotics, P. are difficult treat. Alternative strategies, such as vaccine, needed prevent . We collected total 413 isolates from the blood cerebrospinal fluid patients 10 countries located on 4 continents during 2005–2017 characterized these inform...
Gram-negative bacterial bloodstream infections (GNB-BSI) are common and frequently lethal. Despite appropriate antibiotic treatment, relapse of GNB-BSI with the same strain is associated poor clinical outcomes high healthcare costs. The role persister cells, which sub-populations bacteria that survive for prolonged periods in presence bactericidal antibiotics, unclear. Using a cohort patients relapsed GNB-BSI, we aimed to determine how pathogen evolves within patient between initial...
Salmonella enterica is an important foodborne pathogen that uses secreted effector proteins to manipulate host pathways facilitate survival and dissemination. Different S. serovars cause disease syndromes ranging from gastroenteritis typhoid fever vary in their repertoire. We leveraged this natural diversity identify stm2585, here designated sarA (Salmonella anti-inflammatory response activator), as a induces production of the cytokine IL-10. RNA-seq cells infected with either ΔsarA or...
Significance Staphylococcus aureus (SA) is the leading cause of skin and structure infections (SSSI), which are main portal entry for life-threatening invasive infections. Treatment failures increasingly common due to antibiotic resistance. Importantly, SA SSSI exhibit high 1-year recurrence, despite antibody levels against staphylococcal antigens. Therefore, critical determinants immune protection recurrent not well understood. The present study offers important insights in this area,...
Integrating genomics with growth-based phenotyping reveals divergent roles of antibiotics and incompatibility in plasmid mobility.
The UDP-3- O -( R -3-hydroxyacyl)- N -acetylglucosamine deacetylase LpxC is an essential enzyme in the biosynthesis of lipid A, outer membrane anchor lipopolysaccharide and lipooligosaccharide Gram-negative bacteria. development LpxC-targeting antibiotics toward clinical therapeutics has been hindered by limited antibiotic profile reported non-hydroxamate inhibitors unexpected cardiovascular toxicity observed certain hydroxamate non–hydroxamate-based inhibitors. Here, we report preclinical...
Staphylococcus aureus clonal complex 75 (herein referred to as S. argenteus) lacks the carotenoid pigment operon, crtOPQMN, responsible for production of putative virulence factor, staphyloxanthin. Although a common cause community-onset skin infections among Indigenous populations in northern Australia, this clone is infrequently isolated from hospital-based patients with either bacteremic or nonbacteremic infections. We hypothesized that argenteus would have attenuated compared other...
The species-specific risk of cardiac device-related infection (CDRI) among bacteremic patients is incompletely understood.We conducted a prospective cohort study hospitalized from October 2002 to December 2014 with device (CD) and either Staphylococcus aureus bacteremia (SAB) or Gram-negative (GNB). Cardiac devices were defined as prosthetic heart valves (PHVs), including valvular support rings, permanent pacemakers (PPMs)/automatic implantable cardioverter defibrillators (AICDs), left...
Enterobacter aerogenes was recently renamed Klebsiella . This study aimed to identify differences in clinical characteristics, outcomes, and bacterial genetics among patients with K. versus species bloodstream infections (BSI). We prospectively enrolled or cloacae complex ( Ecc ) BSI from 2002 2015.
Abstract Cefiderocol is a novel catechol siderophore cephalosporin antibiotic developed to treat resistant gram-negative infections. We describe its successful use as rescue therapy, combined with surgical debridement, patient osteomyelitis due extensively drug-resistant Acinetobacter baumannii. Bacterial whole-genome sequencing identified the strain and resistance determinants.
Abstract Background Clinical data characterizing invasive Escherichia coli disease (IED) are limited. We assessed the clinical presentation of IED and antimicrobial resistance (AMR) patterns causative E. isolates in older adults. Methods EXPECT-2 (NCT04117113) was a prospective, observational, multinational, hospital-based study conducted patients with aged ≥ 60 years. determined by microbiological confirmation from blood; or urine an otherwise sterile body site presence requisite criteria...
Abstract Methicillin-resistant Staphylococcus aureus (MRSA) bacteremia is a common and life-threatening infection that imposes up to 30% mortality even when appropriate therapy used. Despite in vitro efficacy determined by minimum inhibitory concentration breakpoints, antibiotics often fail resolve these infections vivo, resulting persistent MRSA bacteremia. Recently, several genetic, epigenetic, proteomic correlates of outcomes have been identified. However, the extent which single...