A5007057300- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Use and Resistance
- Bacterial biofilms and quorum sensing
- Enterobacteriaceae and Cronobacter Research
- Pneumonia and Respiratory Infections
- Vibrio bacteria research studies
- Streptococcal Infections and Treatments
- Infection Control in Healthcare
- Salmonella and Campylobacter epidemiology
- Genomics and Phylogenetic Studies
- Infections and bacterial resistance
- SARS-CoV-2 and COVID-19 Research
- Plant Pathogenic Bacteria Studies
- Microbial Metabolism and Applications
- SARS-CoV-2 detection and testing
- Microbial infections and disease research
- Bacteriophages and microbial interactions
- COVID-19 diagnosis using AI
- Bacterial Infections and Vaccines
- Infective Endocarditis Diagnosis and Management
- Escherichia coli research studies
- Antibiotics Pharmacokinetics and Efficacy
National Health Laboratory Service
2014-2023
University of the Witwatersrand
2014-2023
National Institute for Communicable Diseases
2014-2022
Klebsiella pneumoniae is a common cause of sepsis and particularly associated with healthcare-associated infections. New strategies are needed to prevent or treat infections due the emergence multi-drug resistant K. pneumoniae. The goal this study was determine global diversity distribution O (lipopolysaccharide) K (capsular polysaccharide) antigens on large (>500) collection strains isolated from blood inform vaccine development efforts. A total 645 isolates were collected patients...
Abstract Background Pseudomonas aeruginosa is an opportunistic pathogen that causes a wide range of acute and chronic infections frequently associated with healthcare-associated infections. Because its ability to rapidly acquire resistance antibiotics, P. are difficult treat. Alternative strategies, such as vaccine, needed prevent . We collected total 413 isolates from the blood cerebrospinal fluid patients 10 countries located on 4 continents during 2005–2017 characterized these inform...
Carbapenem-resistant Enterobacteriaceae (CRE) is a concern in South Africa and worldwide. It therefore important that these organisms be accurately identified for infection prevention control purposes.In this study 1193 suspected CREs from 46 laboratories seven provinces were assessed to confirm the prevalence of carbapenemase genes our referral diagnostic isolates period 2012 2015. We compared antimicrobial susceptibility testing method used reference laboratory polymerase chain reaction...
Introduction We aimed to obtain an in-depth understanding on recent antimicrobial resistance trends and molecular epidemiology of S. aureus bacteraemia (SAB). Methods Thirteen academic centres in South Africa were included from June 2010 until July 2012. susceptibility testing was performed the MicroScan Walkaway. Real-time PCR using LightCycler 480 II done for mecA nuc. SCCmec spa-typing finalized with conventional PCR. selected one isolate per common spa type province multilocus sequence...
IntroductionMethicillin-resistant Staphylococcus aureus (MRSA) infections are responsible for longer hospital stays, increased costs, and poorer outcomes compared to methicillin-sensitive S. (MSSA) infections. We aimed describe the epidemiology of bacteraemia (SAB) determine factors associated with MRSA infection in South Africa.MethodsCases SAB were reported from September 2012 2013 three sentinel sites. A case was defined as isolation a blood culture during 21-day period. Detailed clinical...
Background. The increasing rates of antimicrobial resistance observed in the nosocomial pathogen Klebsiella pneumoniae are major public health concern worldwide. Objectives. To describe antibiotic susceptibility profiles K. isolates from bacteraemic patients submitted by sentinel laboratories five regions South Africa mid-2010 to mid-2012. Molecular methods were used detect most commonly found extended-spectrum beta-lactamase (ESBL) and carbapenemase genes. Methods. Thirteen academic centres...
Molecular confirmation of carbapenemase-producing Enterobacteriaceae (CPE) was introduced in South Africa (SA) at the end 2011. We report on detection these resistance genes based referral isolates. with non-susceptibility to any carbapenems according defined criteria for antimicrobial susceptibility testing results were sent a reference laboratory. A proportion isolates had limited demographic, epidemiological and clinical data available. Organism identification reconfirmed using laboratory...
Acinetobacter baumannii is an opportunistic pathogen and causes various infections in patients. This study aimed to describe the clinical, epidemiological molecular characteristics of A. isolated from BCs patients at a tertiary-level hospital South Africa. Ninety-six isolates bloodstream were collected. Clinical recorded patient files. Organism identification AST was performed using automated systems. PCR screening for mcr-1 mcr-5 genes done. To infer genetic relatedness, dendrogram...
Multidrug-resistant (MDR) Gram-negative bacteria are responsible for the majority of healthcare-associated infections and pose a serious threat as they complicate prolong clinical care. A novel cephalosporin-β-lactamase-inhibitor combination, ceftolozane-tazobactam (C/T) was introduced in 2014, which improved treatment MDR pathogens. This study aimed to evaluate activity C/T against Escherichia coli (n = 100), Klebsiella pneumoniae Pseudomonas aeruginosa 100) blood culture isolates South...
Staphylococcus aureus is a healthcare-associated pathogen that can harbour multiple antimicrobial resistance determinants and express virulence factors e.g. Panton-Valentine Leukocidin (PVL). Unknown staphylococcal cassette chromosome mec (SCCmec) typing patterns were previously observed among 11% (n = 52) of methicillin-resistant S. (MRSA) isolates; we further investigated these as well the proportion PVL, encoded by lukS/F-PV, in 761 isolates from patients with diagnosis pneumonia/lower...
Abstract Background The prevalence of Staphylococcus aureus varies depending on the healthcare facility, region and country. To understand its genetic diversity, transmission, dissemination, epidemiology evolution in a particular geographical location, it is important to similarities variations population being studied. This can be achieved by using various molecular characterisation techniques. study aimed provide detailed South African mec A-positive S. blood culture isolates describing...
The emergence and transmission of carbapenem-resistant Enterobacteriaceae (CRE) is a concern in both the clinical public health arenas. Reliable accurate detection these organisms required for patient management infection prevention control purposes. In routine laboratory, phenotypic methods are utilised identification CRE.To investigate profiles suspected carbapenemase-producing (CPE) isolates generated by automated MicroScan Walkaway system making use Clinical Laboratory Standards...
Introduction: Antimicrobial resistant bacterial infections are widespread globally and increases in antimicrobial resistance presents a major threat to public health. Pseudomonas aeruginosa is an opportunistic healthcare-associated pathogen with high rates of morbidity mortality extensive range mechanisms. This study describes the antibiotic susceptibility profiles P. isolates from patients bacteraemia submitted by sentinel laboratories South Africa 2014 2015.
 Methodology: Organism...
The African Journal of Laboratory Medicine focuses on the role laboratory and its professionals in clinical public healthcare sectors, is specifically based an frame reference.
Introduction: Enterobacter cloacae strains have been isolated from Eastern Cape hospitalised patients. Methodology: We molecularly characterised blaOXA-48-, blaIMP- and blaVIM-expressing E. isolates demonstrating resistance to carbapenems five hospitals by multilocus sequence typing. Organism identification antimicrobial susceptibility testing was done using automated systems the were screened for carbapenemases either conventional or real-time PCR then typed Further characterisation of...
We report here the draft genome sequence of Serratia marcescens ML2637, isolated from a South African pediatric patient in intensive care unit with peritoneal cancer. The comprised 5,718,350 bp, 59.1% G+C content. There were 5,594 predicted genes, including 5,301 protein-coding 199 pseudogenes, and 94 RNA genes.
Introduction Methicillin-resistant Staphylococcus aureus (MRSA) is a highly clonal pathogen causing infections in various settings. The aim of this study was to determine if healthcare-associated (HA) MRSA isolates with the same spa -type originating from two geographically distinct hospitals South Africa were genetically related based on PFGE. Furthermore, small subset characterised WGS and then compared PFGE still reliable method define outbreaks and/or transmission chains. Methods...
Acinetobacter baumannii causes invasive paediatric infections, including bacteraemia and meningitis, but neonatal meningitis ventriculitis is uncommon. The treatment of multidrug resistant (MDR) infections often relies on colistin, a polymyxin antibiotic, as last resort. Increased use this drug has led to the emergence colistin resistance. An unusual case colistin-resistant in premature neonate managed with intraventricular described.
We investigated instances of phenotypic and genotypic discrepancies in the identification methicillin-resistant Staphylococcus aureus a reference laboratory South Africa. Organism antimicrobial susceptibility testing were performed using automated systems; and, detection mecA, mecC nuc was real-time conventional PCR methods. Staphylococcal cassette chromosome mec (SCCmec) typing done Ninety-nine percent results correlated; however, there some discrepancies. The gene not detected any isolates...
Raoultella ornitholytica, an environmental organism, has only recently been reported as a cause of infections in humans, and is associated mainly with bacteraemia elderly patients. Carbapenem resistance unusual spp. We describe the first paediatric case blaNDM-1 ornitholytica preterm neonate that developed three days after hospitalisation. This report discusses rare organism had unexpected susceptibility profile caused neonate. Treatment options are explored.