A5085685523- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Pneumonia and Respiratory Infections
- Antibiotic Use and Resistance
- Bacterial Identification and Susceptibility Testing
- Respiratory viral infections research
- Streptococcal Infections and Treatments
- Bacterial Infections and Vaccines
- Fungal Infections and Studies
- Clostridium difficile and Clostridium perfringens research
- Antifungal resistance and susceptibility
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Tuberculosis Research and Epidemiology
- Neonatal and Maternal Infections
- Microscopic Colitis
- SARS-CoV-2 and COVID-19 Research
- COVID-19 epidemiological studies
- COVID-19 Clinical Research Studies
- Bacterial biofilms and quorum sensing
- Nail Diseases and Treatments
- Infections and bacterial resistance
- Mycobacterium research and diagnosis
- Antibiotics Pharmacokinetics and Efficacy
- Clinical Laboratory Practices and Quality Control
University of Limpopo
2016-2024
National Health Laboratory Service
2010-2024
Sefako Makgatho Health Sciences University
2010-2020
Dr George Mukhari Hospital
2020
The Centers
2017
University of Pretoria
2009-2014
Abstract Background We assessed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA shedding duration and magnitude among persons living with human immunodeficiency virus (HIV, PLHIV). Methods From May through December 2020, we conducted a prospective cohort study at 20 hospitals in South Africa. Adults hospitalized symptomatic disease 2019 (COVID-19) were enrolled followed every days nasopharyngeal/oropharyngeal (NP/OP) swabs until documentation of cessation SARS-CoV-2 (2...
The aim of this study was to identify and characterize 97 methicillin-resistant Staphylococcus aureus (MRSA) isolates. Two conventional multiplex PCR assays, a real-time assay two PCR-based genotyping techniques including the spa- hypervariable region (HVR)-typing methods were used MRSA strains isolated between April 2006 September 2007 from Steve Biko Academic Hospital. All isolates positive for 16S rRNA gene, 99% mecA gene 4% Panton-Valentine leukocidin (PVL) gene. Staphylococcal cassette...
IntroductionMethicillin-resistant Staphylococcus aureus (MRSA) infections are responsible for longer hospital stays, increased costs, and poorer outcomes compared to methicillin-sensitive S. (MSSA) infections. We aimed describe the epidemiology of bacteraemia (SAB) determine factors associated with MRSA infection in South Africa.MethodsCases SAB were reported from September 2012 2013 three sentinel sites. A case was defined as isolation a blood culture during 21-day period. Detailed clinical...
Background. The increasing rates of antimicrobial resistance observed in the nosocomial pathogen Klebsiella pneumoniae are major public health concern worldwide. Objectives. To describe antibiotic susceptibility profiles K. isolates from bacteraemic patients submitted by sentinel laboratories five regions South Africa mid-2010 to mid-2012. Molecular methods were used detect most commonly found extended-spectrum beta-lactamase (ESBL) and carbapenemase genes. Methods. Thirteen academic centres...
The incidence of multidrug-resistant tuberculosis (MDR-TB) is increasing and the emergence extensively drug-resistant (XDR-TB) a major challenge. Controlling resistance, reducing transmission improving treatment outcomes in MDR/XDR-TB patients reliant on susceptibility testing. Susceptibility testing using phenotypic methods labour intensive time-consuming. Alternative methods, such as molecular assays are easier to perform have rapid turn-around time. World Health Organization (WHO) has...
The emergence of resistance to multiple antimicrobial agents in pathogenic bacteria is a significant public health threat, as there are limited effective for infections caused by multidrug-resistant (MDR) bacteria. Several MDR now frequently detected. Carbapenem Enterobacteriaceae often plasmid mediated, necessitating stringent infection control practices. We describe an outbreak carbapenem-resistant Providencia rettgeri involving 4 patients admitted intensive care and high-care units at...
The aim of this study was to define factors associated with HIV-infected versus uninfected patients invasive nontyphoidal Salmonella (iNTS) and mortality, which are inadequately described in Africa. Laboratory-based surveillance for iNTS undertaken. At selected sentinel sites, clinical data (age, sex, HIV status, severity illness, outcome) were collected. Surveillance conducted Gauteng, South Africa, from 2003 2013. Clinical microbiological differences between defined risk mortality...
The presence of multi-drug resistant Acinetobacter baumannii raises a big therapeutic challenge in our hospital. Tigecycline, new glycylcycline with expanded broad spectrum activity against organisms was recently licensed South Africa. aim this study to evaluate the vitro tigecycline carbapenem A. complex. Consecutive clinical isolates complex were collected between February and July 2010. Species identification susceptibility testing performed by Vitek-2 colorimetric compact system Advanced...
Abstract In South Africa, 7-valent pneumococcal conjugate vaccine (PCV) was introduced in April 2009 and replaced with 13-valent PCV 2011. We describe the epidemiology of serotype 1 Streptococcus pneumoniae disease during pre- post-PCV eras (2003–2013). Using laboratory-based invasive (IPD) surveillance, we calculated annual incidences, identified IPD clusters, determined 1–associated factors. Of 46,483 cases, 4,544 (10%) were caused by 1. Two clusters infection detected 2003–2004 2008–2012,...
The emergence of resistance to multiple antimicrobial agents in pathogenic bacteria is a significant public health threat, as there are limited effective for infections caused by multidrug-resistant (MDR) bacteria. Several MDR now frequently detected. Carbapenem Enterobacteriaceae often plasmid mediated, necessitating stringent infection control practices. We describe an outbreak carbapenem-resistant Providencia rettgeri involving 4 patients admitted intensive care and high-care units at...
Abstract Background Invasive meningococcal disease (IMD) is endemic to South Africa, where vaccine use negligible. We describe the epidemiology of IMD in Africa. Methods cases were identified through a national, laboratory-based surveillance program, GERMS-SA, from 2003–2016. Clinical data on outcomes and human immunodeficiency virus (HIV) statuses available 26 sentinel hospital sites. conducted space-time analyses detect clusters serogroup-specific cases. Results Over 14 years, 5249...
This study was undertaken to assess the risk of being infected with a known sexually transmitted pathogen at time presentation for termination pregnancy. Endocervical and vaginal swabs were collected diagnosis Neisseria gonorrhoeae, Chlamydia trachomatis Trichomonas vaginalis. Single infections found in 21.5% women, C. commonest (10.0%). Mixed nine trichomoniasis chlamydial six. During speculum examination, discharge observed 73% women. The organism detected patients (11.6%), while T....
Providing country-specific estimates of case fatality and sequelae from bacterial meningitis (BM) is important to evaluate monitor progress toward the World Health Organization's roadmap "defeating by 2030".From 2016-2020, GERMS-SA conducted enhanced surveillance at 26 hospitals across South Africa. Episodes laboratory-confirmed BM due Streptococcus pneumoniae, Haemophilus influenzae, Neisseria meningitidis were included. Risk factors for in-hospital death hospital discharge among survivors...
Introduction: Antimicrobial resistant bacterial infections are widespread globally and increases in antimicrobial resistance presents a major threat to public health. Pseudomonas aeruginosa is an opportunistic healthcare-associated pathogen with high rates of morbidity mortality extensive range mechanisms. This study describes the antibiotic susceptibility profiles P. isolates from patients bacteraemia submitted by sentinel laboratories South Africa 2014 2015.
 Methodology: Organism...
Clostridioides difficile infection (CDI) is a problem in both developed and developing countries common hospital-acquired infection. This guideline provides evidence-based practical recommendations for South Africa other countries. The scope of the includes CDI diagnostic approaches; adult, paediatric special populations treatment options; surveillance prevention control recommendations.
Background: Diagnostic health laboratory services are regarded as an integral part of the national infrastructure across all countries. Clinical tests contribute substantially to system goals increasing quality care and improving patient outcomes.Objectives: This study aimed analyse current expenditures at primary healthcare (PHC) level in South Africa processed by National Health Laboratory Service determine potential cost savings introducing demand management.Methods: A retrospective...
Background. Group B streptococcus (GBS) is a leading cause of invasive disease, particularly in newborns. Seventy-five percent neonates will be colonised by mothers carrying the organism. Confirmation maternal colonisation with GBS essential for prompt treatment and prevention neonatal sepsis. The current gold standard culture isolation has disadvantage long turnaround time (24 - 72 hours). Rapid assays are required to determine carriage GBS. Objectives. To usefulness Xpert technology v....
Background: Typhoid fever remains a public health concern in South Africa, where the risk of transmission is high because poor access to safe water and sanitation. This study describes investigation typhoid outbreak Limpopo province. Methodology: Following notification laboratory-confirmed cases, descriptive was conducted at Sekhukhune District, A suspected case defined as any person residing Makhuduthamaga Municipality from November 2017 January 2018, presenting with gastrointestinal...
The current legislative framework in South Africa (SA) supports adoption as the preferred form of care for children with inadequate or no parental family support. There are an estimated 3.8 million orphans SA, approximately 1.5 - 2 considered adoptable. As a means improving services, newly drafted guidelines from National Department Social Development will future require both non-profit and private sector agencies to obtain medical report on child prior placement. However, local specify what...
Background. COVID-19 has been a major public health concern globally, leading to higher mortality rate, especially among immunosuppressed individuals, who include pregnant women, people with HIV and living other comorbidities. Pregnant women are considered be special population group owing their specific susceptibility some infectious diseases. Objective. To determine the seroprevalence of anti-SARS-CoV-2 antibodies attending an antenatal clinic. Method. This was descriptive cross-sectional...
Background: There is a paucity of research on the incidence and antimicrobial resistance (AMR) Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp. (ESKAPE) pathogens in Africa because inadequate establishment AMR surveillance systems.Objective: This study reports bloodstream ESKAPE at referral hospital northern South Africa.Methods: retrospective descriptive used routinely collected isolates (pathogen...