A5036016675- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Evolution and Genetic Dynamics
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Escherichia coli research studies
Duke University
2017-2025
Lawrence Livermore National Laboratory
2024
Integrating genomics with growth-based phenotyping reveals divergent roles of antibiotics and incompatibility in plasmid mobility.
Abstract Plasmid fitness is directed by two orthogonal processes—vertical transfer through cell division and horizontal conjugation. When considered individually, improvements in either mode of can promote how well a plasmid spreads persists. Together, however, the metabolic cost conjugation could create tradeoff that constrains evolution. Here, we present evidence for presence, consequences, molecular basis conjugation‐growth across 40 plasmids derived from clinical Escherichia coli...
Suppressing plasmid transfer in microbial communities has profound implications due to the role of horizontal gene (HGT) spreading and maintaining diverse functional traits such as metabolic functions, virulence factors, antibiotic resistance. However, existing tools for inhibiting HGT are limited their modes delivery, efficacy, scalability. Here, we present a versatile denial-of-spread (DoS) strategy target eliminate specific conjugative plasmids. Our exploits retrotransfer, whereby an...
Abstract Bacterial evolution through horizontal gene transfer (HGT) reflects their community interactions. In this way, HGT networks do well at mapping interactions, but offer little toward controlling them—an important step in the translation of synthetic strains into natural contexts. Toxin–antitoxin (TA) systems serve as ubiquitous and diverse agents selection; however, utility is limited by erratic distribution hosts. Here we examine heterogeneous TAs a consequence mobility. By...