A5069084967- Cellular Mechanics and Interactions
- Zebrafish Biomedical Research Applications
- Microtubule and mitosis dynamics
- Hippo pathway signaling and YAP/TAZ
- Developmental Biology and Gene Regulation
- RNA Research and Splicing
- Angiogenesis and VEGF in Cancer
- 3D Printing in Biomedical Research
- Wnt/β-catenin signaling in development and cancer
- Microfluidic and Bio-sensing Technologies
- Tissue Engineering and Regenerative Medicine
- Congenital heart defects research
- Axon Guidance and Neuronal Signaling
University of Münster
2014-2015
Paracrine Wnt/β-catenin signalling is important during developmental processes, tissue regeneration and stem cell regulation. Wnt proteins are morphogens, which form concentration gradients across responsive tissues. Little known about the transport mechanism for these lipid-modified in vertebrates. Here we show that Wnt8a transported on actin-based filopodia to contact responding cells activate neural plate formation zebrafish. Cdc42/N-Wasp regulates of Wnt-positive filopodia. Enhanced...
Abstract Tissue vascularization entails the formation of a blood vessel plexus, which remodels into arteries and veins. Here we show, by using time-lapse imaging zebrafish fin regeneration genetic lineage tracing endothelial cells in mouse retina, that vein-derived tip contribute to emerging arteries. Our movies uncover arterial-fated change migration direction migrate backwards within expanding vascular plexus. This behaviour critically depends on chemokine receptor cxcr4a function. We show...
Cell migration and polarization is controlled by signals in the environment. Migrating cells typically form filopodia that extend from cell surface, but precise function of these structures guided poorly understood. Using vivo model zebrafish primordial germ for studying chemokine-directed single migration, we show distribution their dynamics are dictated gradient chemokine Cxcl12a. By specifically interfering with formation, demonstrate first time protrusions play an important role Cxcl12a,...
Abstract The precise positioning of organ progenitor cells constitutes an essential, yet poorly understood step during organogenesis. Using primordial germ that participate in gonad formation, we present the developmental mechanisms maintaining a motile cell population at site where develops. Employing high-resolution live-cell microscopy, find repulsive cues coupled with physical barriers confine to correct bilateral positions. This analysis revealed polarity changes on interaction barrier...
Significance Cell motility is critical for a wide range of processes in development, homeostasis, and immune response. Conversely, abnormal regulation cell migration leads to pathological consequences like cancer metastasis. In this study, we investigate the mechanisms controlling timing acquisition, using zebrafish primordial germ cells as an vivo model. We defined previously unknown role signaling scaffold molecule regulator Gα protein Rgs14a coordinating onset with presence guidance cues....