A5107989040- Pluripotent Stem Cells Research
- Developmental Biology and Gene Regulation
- MicroRNA in disease regulation
- Zebrafish Biomedical Research Applications
- RNA Research and Splicing
- Microfluidic and Bio-sensing Technologies
- Immunotherapy and Immune Responses
- 3D Printing in Biomedical Research
- Animal Genetics and Reproduction
- Molecular Biology Techniques and Applications
- RNA Interference and Gene Delivery
- Congenital heart defects research
- Cellular Mechanics and Interactions
Institute of Molecular Biology
2013-2015
University of Münster
2014
Whole-mount in situ hybridization (WISH) is a fundamental tool for studying the spatio-temporal expression pattern of RNA molecules intact embryos and tissues. The available methodologies detecting mRNAs rely on enzymatic activities chemical reactions that generate diffusible products, which are not fixed to detected RNA, thereby reducing spatial resolution technique. In addition, current WISH techniques time-consuming usually combined with methods reporting protein molecules. protocol we...
Abstract The precise positioning of organ progenitor cells constitutes an essential, yet poorly understood step during organogenesis. Using primordial germ that participate in gonad formation, we present the developmental mechanisms maintaining a motile cell population at site where develops. Employing high-resolution live-cell microscopy, find repulsive cues coupled with physical barriers confine to correct bilateral positions. This analysis revealed polarity changes on interaction barrier...
Significance Cell motility is critical for a wide range of processes in development, homeostasis, and immune response. Conversely, abnormal regulation cell migration leads to pathological consequences like cancer metastasis. In this study, we investigate the mechanisms controlling timing acquisition, using zebrafish primordial germ cells as an vivo model. We defined previously unknown role signaling scaffold molecule regulator Gα protein Rgs14a coordinating onset with presence guidance cues....
Whole-mount in situ hybridization (WISH) is a fundamental tool for studying the spatio-temporal expression pattern of RNA molecules intact embryos and tissues. The available methodologies detecting mRNAs rely on enzymatic activities chemical reactions that generate diffusible products, which are not fixed to detected RNA, thereby reducing spatial resolution technique. In addition, current WISH techniques time-consuming usually combined with methods reporting protein molecules. protocol we...