A5047454757- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Genetic and phenotypic traits in livestock
- Effects of Environmental Stressors on Livestock
- Animal Genetics and Reproduction
- Ovarian function and disorders
- Reproductive biology and impacts on aquatic species
- Veterinary Equine Medical Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Animal Nutrition and Physiology
- Male Reproductive Health Studies
- Selenium in Biological Systems
- Rabbits: Nutrition, Reproduction, Health
- Hormonal and reproductive studies
- Animal health and immunology
- Reproductive Health and Technologies
- Veterinary Medicine and Surgery
- Aquaculture Nutrition and Growth
- Antioxidant Activity and Oxidative Stress
- Sexual Differentiation and Disorders
- Bee Products Chemical Analysis
- Agricultural and Food Sciences
- MicroRNA in disease regulation
- Animal Behavior and Welfare Studies
Universidade de São Paulo
2016-2025
Universidade Brasil
2010-2025
Universidade Federal de Uberlândia
2011
Abstract Semen fertilizing potential is dependent upon the morphological, functional and molecular attributes of sperm. Sperm microRNAs (miRNAs) were recently shown to hold promise regarding their association with different fertility phenotypes. However, role in regulation remains be determined. We postulated that sperm miRNAs might regulate early embryonic development. From this perspective, quality 380 investigated frozen–thawed semen from high (HF; 54.3 ± 1.0% pregnancy rate) low (LF;...
ABSTRACT The aim is to report a case of ovarian hematoma in female equine, Brazilian Sport Horse breed, 5 years old. mare was her third breeding season, with history good fertility previous seasons. During reproductive monitoring, the animal showed regular growth follicles. In presence dominant follicle (44x39mm) right ovary, synthetic prostaglandin administered. After 24 hours, ovary an increase size and sensitivity. On ultrasound, well-defined echogenic structure (66x54mm) observed,...
Detection of reactive oxygen species (ROS) is great interest in semen analysis since their excess detrimental to sperm function and male fertility. Fluorescence microscopy has achieved attention for providing broad possibilities evaluations also presenting substantial accessibility. In this context, study investigated the efficiency CellROX Deep Red® Orange® probes detecting ROS bovine cells assessed relationship with fertility potential. First, 16 ejaculates were assigned three treatments:...
The objective was to evaluate the suitability of using natural or lyophilized low density lipoproteins (LDL), in lieu whole egg yolk, extenders for cryopreserving ram semen. Once extragonadal sperm reserves were depleted 10 fertile Santa Inês cross rams, two ejaculates per collected cryopreservation. Nine used: Tris-16% yolk extender with 5% glycerol as a control (T1), and substitution 8, 12, 16 20% LDL (T2-T5, respectively), 16, (T6-T9). Semen diluted 100 × 10(6) sperm/mL, packaged into...
In this experiment, it was defined a protocol of fluorescent probes combination: propidium iodide (PI), fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FITC-PSA), and JC-1. For purpose, four ejaculates from three different rams (n=12), all showing motility >80% abnormal morphology <10%, were diluted in TALP medium split into two aliquots. One the aliquots flash frozen thawed continuous cycles, to induce damage cellular membranes disturb mitochondrial function. Three...
In order to provide information that may help researchers understand the main cause(s) of differences in bull fertility frequently observed field trials, this study aimed investigate conception rates as well several vitro sperm characteristics different sires unknown utilized a Timed-AI (TAI) program. Suckled Nelore cows submitted same TAI protocol were allocated into eight breeding groups approximately 120 animals each. Frozen semen doses from three Angus bulls and batches each utilized....
The objective was to verify the relationship between equine semen cryopreservation and changes related increased lipid peroxidation. Also, addition of autologous or homologous seminal plasma from a stallion with good freezing response post-thawed sperm tested determine whether it would confer protection. Frozen-thawed were evaluated allocated into three groups: without addition, supplemented either plasma. All groups at 0, 60 120 min after incubation 37 °C. Cryopreservation did not increase...
The objective of this study was to evaluate the quality bovine frozen-thawed sperm cells after Percoll gradient centrifugation. Frozen semen doses were obtained from six bulls different breeds, including three taurine and Zebu animals. Four ejaculates per bull evaluated before discontinuous Sperm motility assessed by computer-assisted analysis integrity plasma acrosomal membranes, as well mitochondrial function, using a combination fluorescent probes propidium iodide, fluorescein...
The objective was to determine the effect of sequence insemination after simultaneous thawing multiple 0.5 mL semen straws on conception rate in suckled multiparous Nelore cows. this procedure vitro sperm characteristics also evaluated. All cows (N = 944) received same timed AI protocol. Ten (0.5 mL) frozen from batch were simultaneously thawed at 36 °C, for a minimum 30 sec. One straw per cow used AI. Frozen three Angus bulls used. Timed records included (first tenth) and time removal bath....
Reactive oxygen species (ROS) can induce sperm damage, especially after cryopreservation. Melatonin (MEL) is a potent amphiphilic antioxidant, allowing free penetration to any compartment of cell. Ferulic acid (FA) phenolic compound exhibiting wide range beneficial effects against several pathologies due its antioxidant property. This study aimed evaluate the effect MEL and FA on quality cryopreserved equine semen. Five ejaculates from four stallions were collected. For each ejaculate,...
The objective of this study was to characterize acrosomal ultrastructure following discontinuous Percoll gradient centrifugation cryopreserved bovine sperm. Semen collected from six bulls different breeds and three ejaculates per bull were evaluated. Frozen semen samples thawed the region sperm cells evaluated by transmission electron microscopy (TEM) before (n = 18) after centrifugation. evaluation 20 heads each 36 analyzed ensured that a large number investigated. data subjected analysis...