A5068999945- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive Physiology in Livestock
- Reproductive Health and Technologies
- Demographic Trends and Gender Preferences
- Capital Investment and Risk Analysis
- Genetic and phenotypic traits in livestock
- Urological Disorders and Treatments
- Male Reproductive Health Studies
- Plant Disease Resistance and Genetics
- Reproductive biology and impacts on aquatic species
- Genetically Modified Organisms Research
- Innovation Policy and R&D
Universidade Salvador
2017-2020
Universidade Federal da Bahia
2010-2017
Hospital Ana Nery
2012-2017
Universidade Estadual Paulista (Unesp)
2008-2011
Hospital Universitário da Universidade de São Paulo
2011
The objective of this study was to evaluate the quality bovine frozen-thawed sperm cells after Percoll gradient centrifugation. Frozen semen doses were obtained from six bulls different breeds, including three taurine and Zebu animals. Four ejaculates per bull evaluated before discontinuous Sperm motility assessed by computer-assisted analysis integrity plasma acrosomal membranes, as well mitochondrial function, using a combination fluorescent probes propidium iodide, fluorescein...
The purpose of this work was to associate the modified swim-up method with centrifugation in density gradient for separation X-bearing spermatozoa. Sperm viability and integrity were evaluated through Trypan Blue/Giemsa staining method. Quality control centrifuged spermatozoa performed vitro produced embryos. results validated by sex ratio embryos using PCR Y- specific sequences present bovine male genomic DNA. After determining genetic embryos, showed difference (P<0.05) deviation when...
The objective of the present study was to determine sperm enrichment with X-bearing spermatozoa, after one centrifugation in a Percoll or OptiPrep continuous density gradient, using quantitative real-time polymerase chain reaction (qPCR) DNA and resultant vitro-produced bovine embryos by PCR. Frozen/thawed layered on gradients tubes were centrifuged. Supernatants gently aspirated recovered from bottom tubes. Cleavage blastocyst rates determined through vitro production PCR performed identify...
The effectiveness of induction the acrosome reaction (AR) test as a parameter to in vitro estimate embryo production (IVP) Nelore breed and AR pattern by Trypan Blue/Giemsa (TB) stain were evaluated. Frozen semen samples from ten bulls submitted also evaluated for cleavage blastocyst rates. treatments utilized were: control (TALP medium), TH medium + 10μg heparin), TL 100μg lysophosphatidylcholine) THL heparin lysophosphatidylcholine). Sperm acrosomal status viability TB staining at 0 after...
O objetivo neste trabalho foi desenvolver um método de seleção do sexo espermatozoides bovinos por centrifugação em gradiente densidade Percoll. Utilizou-se sêmen congelado touros mantidos regime colheita sêmen. A fração X ou Y separada treze diferentes gradientes Percoll formados 1 a 12 camadas com densidades que variaram 1,004 g/mL 1,123 g/mL. As soluções foram preparadas misturando-se, proporções diferentes, meio cultura Hank's e uma solução estoque composta NaCl 1,5 M (1:9, v/v). Sobre...
The aim of this study was to separate X-bearing bovine sperm by continuous Percoll and OptiPrep density gradients validate the sexing resultant in vitro produced embryos Polimerase Chain Reaction (PCR). Frozen/thawed layered on which were previously prepared polystyrene tubes, 24 h before procedures maintained at 4 °C. tubes centrifuged 500 x g for 15 min 22 Supernatants gently aspirated recovered from bottom tubes. Viability integrity evaluated Trypan Blue/Giemsa stain. Cleavage blastocyst...
Embriões bovinos produzidos in vitro, em estádio de mórula, foram cultivados meio contendo anticorpos anti H-Y alto título proveniente ratos por 24h e, após este tempo, classificados dois grupos: 1) embriões inibidos mórula (classificados como machos) e 2) que se desenvolveram formaram a blastocele fêmeas). O sexo 311 embriões, distribuídos três grupos concentração dos anticorpos, 3%, 5% ou 7%, foi identificado pela reação cadeia da polimerase. Não houve desvio proporção entre machos fêmeas...
The low cost of sperm sexing methods combined with in vitro embryo production genetic improvement programs can increase the profitability cattle production, particular when it does not decrease reproductive efficiency. aim this work was to evaluate sex ratio deviation thawed bovine semen processed by density gradient centrifugation and swim-up. Semen doses were collected from ten bulls different breeds, each experimental group replicated times.A Percoll™ prepared mixing Dulbecco’s Modified...
Este trabalho teve como objetivo avaliar a eficiência de gradientes densidade contínuos Percoll e OptiPrep para separação espermatozoides portadores do cromossomo X, influência sobre viabilidade espermática, taxa prenhez proporção sexo. Para isso, 100 milhões foram depositados em cada tubo contendo o gradiente densidade, previamente preparados, tubos poliestireno, 24 horas antes da centrifugação mantidos 4 °C. Centrifugou-se 500 x g por 15 minutos 22 °C e, posteriormente, os sobrenadantes...
The embryo transfer is becoming a widespread practice. Most embryos are collected from spontaneous single ovulating mares and result in 50% of recovery, increasing the costs production. To illustrate, price catheter for recovery range US$ 194.00 to 250.00 (R$ 350.00 R$ 450.00). Therefore, aim this work was verify if with damaged balloon can be recuperated reused without altering its efficiency. For study, two groups were used: control group (GI, n=10), on which nonsurgical performed original...
Resumo Objetivou-se determinar o efeito e melhor nível de inclusão ácido ascórbico em meio diluidor TRIS-Gema para criopreservação sêmen caprino. Foram utilizados cinco ejaculados três bodes da raça Anglo Nubiana. Cada ejaculado foi dividido quatro alíquotas 200 µL, compondo tratamentos: um sem adição (controle) os demais com 0,0528; 0,1056 0,1584mg/mL no TRIS-Gema. Avaliaram-se motilidade espermática progressiva vigor espermático pós-diluição, pós-resfriamento pós-descongelamento;...
Objetivo do presente trabalho foi comparar a performance de sêmen cachorro in vitro e vivo com soro albumina bovina – tris (TB) diluente gema ovo Tris (TR). Para isso, foram usados vinte cães como doadores. O dos colhido via massagem peniana. Semanalmente fração rica espermática colhida até quarenta ejaculados. Após o exame macroscópico microscópico, as amostras diluídas em partes iguais cada ejaculado (TB TE), pelo método one-step 32º C. embalado palhetas francesas 0,5 mL, quais...