A5000270232- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Xenotransplantation and immune response
- Sperm and Testicular Function
- Congenital Anomalies and Fetal Surgery
- Prenatal Screening and Diagnostics
- Bee Products Chemical Analysis
- Ovarian function and disorders
- Animal health and immunology
- Renal and related cancers
- Reproductive Health and Technologies
- Toxoplasma gondii Research Studies
- Genetically Modified Organisms Research
- Meat and Animal Product Quality
- Veterinary Oncology Research
- Reproductive Physiology in Livestock
- Clinical Laboratory Practices and Quality Control
- Veterinary Equine Medical Research
- Seed Germination and Physiology
- Ultrasound and Hyperthermia Applications
- T-cell and Retrovirus Studies
- Organ Transplantation Techniques and Outcomes
- Virus-based gene therapy research
- Additive Manufacturing Materials and Processes
- Rural Development and Agriculture
Universidade Federal Rural do Semi-Árido
2011-2022
Universidade Estadual Paulista (Unesp)
2010
Nortel (Canada)
2006
Hematological and biochemical profile studies help to evaluate functional changes of animals used in experiments. The aim this study was determine the hematological immunosuppressed BALB/c nude C57BL/6 SCID mice after bovine ovarian xenotransplantation. Therefore, a total 74 female were divided into four groups: non-xenotransplanted animals, xenotransplanted treated with eCG FSH + LH. After anesthesia, blood samples collected hematologic values evaluated. results showed no significant...
Background: Animal models are widely used in scientific research because of the ability to generate information from an organism like everything under a given experimental condition. Hematological and biochemical tests laboratory animals essential for validation several studies. In addition, it standardizes physiological values these according their sex, age, lineage, environment, nutritional status. The present work aims establish reference hematological standards Balb/c mice, males...
The aim of the present study was to evaluate development fresh and vitrified agouti ovarian tissue after xenografting C57Bl/6 severe combined immunodeficiency (SCID) female mice. Ovaries were obtained from five agoutis divided into 16 fragments. Five fragments transplanted immediately ovariectomised SCID mice others vitrified, stored for 2 weeks only rewarming. Tissue under kidney capsule in recipients. return activity recipients monitored by observation external signs oestrus vaginal...
Descreve-se a técnica de biópsia hepática com agulha tru-cut guiada por videolaparoscopia em 12 caprinos machos, castrados, hígidos, sem raça definida, distribuídos dois grupos (G): G1, cinco animais meses e G2, sete seis idade. O procedimento foi realizado sob anestesia geral intravenosa o animal decúbito lateral esquerdo. pneumoperitônio laparoscopia foram procedidos no flanco direito, aproximadamente 10cm ventral aos processos transversos das vértebras lombares. A introduzida 11º espaço...
The aim of this study was to separate X-bearing bovine sperm by continuous Percoll and OptiPrep density gradients validate the sexing resultant in vitro produced embryos Polimerase Chain Reaction (PCR). Frozen/thawed layered on which were previously prepared polystyrene tubes, 24 h before procedures maintained at 4 °C. tubes centrifuged 500 x g for 15 min 22 Supernatants gently aspirated recovered from bottom tubes. Viability integrity evaluated Trypan Blue/Giemsa stain. Cleavage blastocyst...
RESUMO O presente trabalho objetivou estudar a ocorrência de surtos naturais fotossensibilização que vêm ocorrendo em diversas espécies animais na região semi-árida do Estado Rio Grande Norte. Foram visitadas 17 propriedades nas quais estavam casos desta patologia. realizadas entrevistas com os tratadores, exames clínicos e provas séricas bioquímicas função hepática nos afetados. Também foram avaliações das pastagens para busca plantas suspeitas causarem doença. Essasa mostras testadas...
The aim of this study was to assess a vitrification protocol for asinine ovarian tissue, preserve preantral follicles using different cryoprotectant solutions, composed various concentrations (EG 3 M or 6 M) dimethyl sulfoxide ethylene glycol isolate, as combination (DMSO + EG M). Ten pairs ovaries from Brazilian north-eastern breed jennies were obtained through videolaparoscopy, and cortical fragments submitted solid-surface (SSV) each solution. tissue evaluated follicular morphology...
Domestic and wild goats are very susceptible animals to predation, specially when pregnancy occurs. This study aimed evaluate the use of goat fetal ovarian tissue for vitrification followed by xenotransplantation fresh in two immunosuppressed mice models (C57BL/6 SCID Balb-C NUDE). Goat fetus ovaries were collected slaughterhouses, divided into small cortical pieces destined (FX) cryopreservation (CX). Five recipients from each lineage used FX 10 CX. The euthanized after 65 postoperative...
ABSTRACT: This study aimed to evaluate the characteristics of two different murine models hormone-treated renal-encapsulated bovine ovarian tissue xenotransplantation. Two immunodeficient mouse (BALB/c Nude and C57BL6 SCID) were xenografted with pieces from heifers each group was subjected hormonal treatments eCG or a combination FSH+LH. Donor ovaries recipients evaluated by histology infrared thermography at times. At time xenograft collection, animals for alterations in hepatorenal...
This study evaluated the effect of extract Aloe vera at concentrations 10% and 20% on cryopreservation sperm from epididymis domestic cats. Epididymal spermatozoa were recovered using flotation technique used in treatments: control (TRIS-egg yolk 20%), T10% (TRIS plus A. extract), T20% extract). The subjected to 4ºC for 60 minutes, followed by 20 minutes nitrogen vapors, stored a cryogenic cylinder. samples thawed 37°C 30 seconds. motility decreased (P<0.05) after thawing three treatments....
This study assessed the hoof balance of 50 adult Quarter horses in training vaquejada to determine biometry and incidence imbalance. Seven measurements were taken: angle at toe, lateral medial heel length, toe frog length width, width sole. From these following problems could be identified: 32 out (64%) had contracted heels, 15 (30%) imbalance, 27 (54%) asymmetry hoof. The difference among contralateral members was not significant, however, some presented opposite forelimb, varying from...
O cultivo de células animais existe há cerca 100 anos. Os estudos pioneiros pesquisadores como Harrison, Carel e Roux, contribuíram para o desenvolvimento da técnica encorajamento outros trabalhos relacionados ao células. Cultivo pode ser definido um conjunto práticas que permitem a manutenção em sistema in vitro independente do tecido origem sob condições controladas. primeiros cultivos resumiam-se tecidos fragmentados submersos plasma sanguíneo (ou soro animal origem) contidos frascos....
O presente artigo objetiva realizar breve revisao sobre os principais agentes infecciosos de abortos em caprinos criados no Nordeste brasileiro, e, apontar a prevalencia sorologica tais microrganismos. Na literatura consultada foram observados registros pelas bacterias Leptospira spp. e Chlamydophila abortus; pelos protozoarios Neospora caninum, Toxoplasma gondii Trypanosoma vivax. Nao houve relatos da ocorrencia do componente viral na etiologia dos brasileiro. As enfermidades causadas por...
O transplante ovariano constitui-se numa tecnica em que um ovario ou parte dele e transferido de doador para receptoras podem ser o mesmo individuo, outro da mesma especie ainda diferente, locais proximos distantes posicao anatomica original. A presente revisao aborda conceitos importantes a elucidacao dos principais eventos foliculogenese maturacao folicular bem como, as aplicacoes transplantes ovarianos perspectivas dessa na reproducao animais domesticos, silvestres humanos. Outros estudos...
O cultivo de celulas animais existe ha cerca 100 anos. Os estudos pioneiros pesquisadores como Harrison, Carel e Roux, contribuiram para o desenvolvimento da tecnica encorajamento outros trabalhos relacionados ao celulas. Cultivo pode ser definido um conjunto praticas que permitem a manutencao em sistema in vitro independente do tecido origem sob condicoes controladas. primeiros cultivos resumiam-se tecidos fragmentados submersos plasma sanguineo (ou soro animal origem) contidos frascos....
Xenotransplante consiste em transplantar orgaos, tecidos e/ou celulas de animais, geneticamente modificados ou nao, entre especies diferentes. O xenotransplante ovariano e utilizado como metodo coleta maturacao dos foliculos seus oocitos, para tanto necessario ainda aprimoramento algumas tecnicas. Este artigo revisao tem objetivo, abordar aspectos fisiologicos foliculares registrar o aperfeicoamento da tecnica que forma os pesquisadores vem conduzindo estudos nos ultimos anos animal.