A5027819447- Reproductive Physiology in Livestock
- Genetic and phenotypic traits in livestock
- Reproductive Biology and Fertility
- Effects of Environmental Stressors on Livestock
- Ruminant Nutrition and Digestive Physiology
- Ovarian function and disorders
- Reproductive System and Pregnancy
- Orthopedic Surgery and Rehabilitation
- Animal Nutrition and Health
- Tendon Structure and Treatment
- Agronomic Practices and Intercropping Systems
- Agricultural Innovations and Practices
- Bioenergy crop production and management
- Animal Genetics and Reproduction
- Surgical Sutures and Adhesives
- Agriculture and Farm Safety
- Animal health and immunology
- Sperm and Testicular Function
- Diverse Educational Innovations Studies
- Livestock Management and Performance Improvement
University of Wisconsin–Madison
2018-2024
Michigan State University
2011-2021
University of California Division of Agriculture and Natural Resources
2018-2019
The objective of this experiment was to determine the effect high versus low progesterone (P4) during pre-dominance or dominance phase (or both) ovulatory follicle development on follicular dynamics and fertility lactating dairy cows. Progesterone manipulated reach (H) (L) serum concentrations (d 0 4 wave) 5 7 a second wave follicle, creating treatments: H/H, H/L, L/H, L/L. Luteolysis induced with PGF2α d ovulation GnRH 56 h after PGF2α. Cows (n = 558) received artificial insemination (AI)...
Because progesterone (P4) is essential for pregnancy establishment and maintenance, we investigated the effect of increased concentrations P4 on embryonic attachment pregnancy-associated glycoproteins (PAG). Additionally, relationships among luteal regression, loss, PAG in cows undergoing loss by d 33 pregnancy. Lactating dairy were allocated into control (n = 40) human chorionic gonadotropin (hCG; 3,300 IU 7 13 to promote greater circulating P4; GnRH 0; n 46) groups. Progesterone was...
Our objective was to determine the effect of inducing an accessory corpus luteum (CL) with human chorionic gonadotropin (hCG; 3,300 IU) on d 7 (hCG7) or 2 CL hCG and 13 (hCG7+13) estrous cycle in noninseminated lactating Holstein cows. Cows (n = 86) between 39 64 DIM were pretreated Ovsynch + CIDR protocol, only synchronized cows used 64). The day last GnRH considered 0 cycle. Follicular luteal dynamics evaluated daily during entire by ovarian ultrasonography. Blood samples collected measure...
Pregnancy loss (PL) in lactating dairy cows disrupts reproductive and productive efficiency. We evaluated the expression of interferon-stimulated genes (ISG) blood leukocytes, vaginal cervical epithelial cells, luteolysis-related genes, progesterone, pregnancy-associated glycoprotein (PAG) profiles (n = 86) to gain insight about PL. Expression ISG on d17, d19, d21 was greater that maintained pregnancy (P33) compared nonpregnant with no PL (NP). Greater differences between groups were...
The objective was to determine the effect of sequence insemination after simultaneous thawing multiple 0.5 mL semen straws on conception rate in suckled multiparous Nelore cows. this procedure vitro sperm characteristics also evaluated. All cows (N = 944) received same timed AI protocol. Ten (0.5 mL) frozen from batch were simultaneously thawed at 36 °C, for a minimum 30 sec. One straw per cow used AI. Frozen three Angus bulls used. Timed records included (first tenth) and time removal bath....
Our objective was to evaluate the effect of treatment with human chorionic gonadotropin (hCG) at time transfer in vitro produced (IVP) beef embryos on pregnancy outcomes lactating multiparous Jersey cows. Grade 1, Stage 7 (expanded blastocyst), IVP were from black Angus-based dams using 3 proven high fertility Angus sires and frozen for direct transfer. In a preliminary experiment, cows randomized 2x2 factorial arrangement treatments test main recipient synchronization protocol...
This study aimed to determine the effect of 2 simple breeding strategies combining artificial insemination (AI) after detection estrus (AIED) and timed AI (TAI) on first-service fertility in lactating Holstein cows. Weekly, cows (n = l,049) between 40 46 d milk (DIM) were randomly assigned initiate 1 for first service: Presynch-14 PG+G. is a presynchronization strategy with PGF2α treatments 14 apart last before initiation Ovsynch protocol. Cows treated PG+G receive simpler program that uses...
The objective was to determine the effect of inducing an accessory corpus luteum (CL) using GnRH or human chorionic gonadotropin (hCG) on day in vitro-produced (IVP) embryo transfer (ET) pregnancy per ET (P/ET) and calving/ET dairy heifers lactating cows. Dairy (11-15 mo age; n = 1,547) cows (n 1,480) detected estrus by tail chalk (d 0) were used as recipients. Before ET, presence a CL evaluated transrectal palpation from d 6 9 estrous cycle. Animals with randomly assigned receive 1 3...
Cloning cattle using somatic cell nuclear transfer (SCNT) is inefficient. Although the rate of development SCNT embryos in vitro similar to that fertilized embryos, most fail develop into healthy calves. In this study, we aimed identify developmentally competent according blastocyst composition and perform transcriptome analysis single embryos. Transgenic expressing nuclear-localized HcRed gene at day 7 were imaged by confocal microscopy for counting individually transferred recipient...
This study aimed to determine if 200 μg of GnRH (gonadorelin hydrochloride) would increase ovulatory response and pregnancies per AI (P/AI) compared with 100 at the first breeding-Ovsynch a Double-Ovsynch program (DO) in lactating Holstein cows. Weekly cohorts primiparous (n = 719) multiparous 1,191) cows submitted DO [GnRH – 7 d PGF2α 3 (G1) (PG1) 1 ~32 h (G2) ~16 TAI] for timed-artificial insemination (TAI), randomly received either or G1 (primiparous, 64–75 DIM; multiparous, 59–70 DIM)....
We investigated the effect of an additional GnRH 2 d after initiation resynchronization on 25 post-AI proportion cows without a corpus luteum (CL) at nonpregnancy diagnosis (NPD) and pregnancy per AI (P/AI) with CL NPD that were reinseminated. Once week, inseminated earlier randomly assigned to receive either treatment only (G25) or this day later (G25&27). Pregnancy presence determined 32 using ultrasonography. Nonpregnant enrolled in different strategies for ovulation according parity...
Artificial insemination 12 h following observed standing estrus is a standard estimate of the fertility levels cattle. The main objective this study was to determine if controlling ovarian development with program could alter lactating dairy cows. Lactating cows (n = 1356) 60–66 days in milk (DIM) were randomly assigned receive timed AI Presynch-11/Ovsynch (Presynch-11) or combination detection and using (Estrus + TAI). Cows estrus, first two cloprostenol sodium (CLO) injections, Estrus TAI...