A5084944533- 3D Printing in Biomedical Research
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Dermatology and Skin Diseases
- Skin Protection and Aging
- Cancer and Skin Lesions
- Viral Infectious Diseases and Gene Expression in Insects
- Ion Channels and Receptors
- Skin and Cellular Biology Research
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Circadian rhythm and melatonin
- Advanced Drug Delivery Systems
- Wound Healing and Treatments
- Hair Growth and Disorders
- Pharmacological Effects of Natural Compounds
- Exercise and Physiological Responses
- Advancements in Transdermal Drug Delivery
- Cellular Mechanics and Interactions
Amorepacific (South Korea)
2018-2024
L'Oréal (France)
2021
Centre Hospitalier Universitaire de Lille
2021
Rady Children's Hospital-San Diego
2015
University of California, San Diego
2015
Universidade Federal do Rio de Janeiro
2009-2012
Future clinical applications of human embryonic stem (hES) cells will require high-yield culture protocols. Currently, hES are mainly cultured in static tissue plates, which offer a limited surface and repeated sub-culturing. Here we describe stirred system with commercial dextran-based microcarriers coated denatured collagen to scale-up cell production. Maintenance pluripotency the microcarrier-based was shown by immunocytochemical flow cytometry analyses for pluripotency-associated...
Vascularization of reconstructed tissues is one the remaining hurdles to be considered improve both functionality and viability skin grafts relevance ofin vitroapplications. Our study, therefore, sought develop a perfusable vascularized full-thickness equivalent that comprises more complex blood vasculature compared existing models. We combined molding, auto-assembly microfluidics techniques in order create representing (a) differentiated epidermis with physiological organization correctly...
Abstract Human pluripotent stem cells (hPSC) are used to study the early stages of human development in vitro and, increasingly due somatic cell reprogramming, cellular and molecular mechanisms disease. Cell culture medium is a critical factor for hPSC maintain pluripotency self-renewal. Numerous defined media have been empirically developed but never systematically optimized culturing hPSC. We applied design experiments (DOE), powerful statistical tool, improve formulation Using growth as...
Currently, stem cell research faces a major bottleneck related to the low efficiency of methods produce large quantities human embryonic cells (ESC) for use in clinical trials. Most culture media currently employed ESC cultivation contain animal compounds, and are grown static flasks. Besides immediate contamination with nonhuman expansion flasks tends be laborious nonefficient. Here we cultured stirred microcarrier (MC) systems using an animal/human-component-free medium, overcome both...
Mannosylerythritol lipids (MELs) are glycolipids and have several pharmacological efficacies. MELs also show skin-moisturizing efficacy through a yet-unknown underlying mechanism. Aquaporin-3 (AQP3) is membrane protein that contributes to the water homeostasis of epidermis, decreased AQP3 expression following ultraviolet (UV)-irradiation skin associated with reduced moisture. No previous study has examined whether effect might act modulation expression. Here, we report for first time...
Abstract The development of efficient and reproducible culture systems for embryonic stem (ES) cells is an essential pre‐requisite regenerative medicine. Culture scale‐up ensuring maintenance cell pluripotency a central issue, because large amounts pluripotent must be generated to warrant that differentiated deriving thereof are transplanted in great survive the procedure. This study aimed develop robust scalable expansion system, using murine line feeder‐dependent adapted serum‐free medium,...
Abstract In vitro hair follicle (HF) models are currently limited to ex vivo HF organ cultures (HFOCs) or 2D that of low availability and do not reproduce the architecture behavior hair, leading poor screening systems. To resolve this issue, we developed a technology for construction human in construct based on assemblage different types cells present organ. First, demonstrated epithelial cells, when isolated , have similar genetic signatures regardless their dissection site, trichogenic...
Seasonal changes can affect the physiological condition of skin and cause various cutaneous disorders. The barrier function tends to worsen during winter when humidity is lower compared other seasons. To determine influence relative (RH) on barrier, we performed biological histological assays using equivalents that were cultured under reduced in an environmental chamber. We found led decreased epidermal thickness disruption barrier. Reduced induced decrease filaggrin, loricrin damage tight...