A5015652543- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Psychedelics and Drug Studies
- Pharmacological Receptor Mechanisms and Effects
- Infectious Encephalopathies and Encephalitis
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Biocrusts and Microbial Ecology
- Fatty Acid Research and Health
- Marine Toxins and Detection Methods
- Mosquito-borne diseases and control
- Down syndrome and intellectual disability research
- Cannabis and Cannabinoid Research
- Alzheimer's disease research and treatments
- Biochemical Analysis and Sensing Techniques
- Neurogenesis and neuroplasticity mechanisms
- Tryptophan and brain disorders
- Aquatic Ecosystems and Phytoplankton Dynamics
- Peroxisome Proliferator-Activated Receptors
- Cholesterol and Lipid Metabolism
- Pluripotent Stem Cells Research
- Folate and B Vitamins Research
- Connective tissue disorders research
- Mitochondrial Function and Pathology
D’Or Institute for Research and Education
2018-2024
Universidade Federal do Rio de Janeiro
2018-2022
Universidade do Estado do Rio de Janeiro
2021
Institute of Biomedical Science
2020
Abstract Zika virus (ZIKV) has been extensively studied since it was linked to congenital malformations, and recent research revealed that astrocytes are targets of ZIKV. However, the consequences ZIKV infection, especially this cell type, remain largely unknown, particularly considering integrative studies aiming understand crosstalk among key cellular mechanisms fates involved in neurotoxicity virus. Here, infection iPSC-derived presented. Our results show ROS imbalance, mitochondrial...
The northeast (NE) region of Brazil commonly goes through drought periods, which favor cyanobacterial blooms, capable producing neurotoxins with implications for human and animal health. most severe dry spell in the history occurred between 2012 2016. Coincidently, highest incidence microcephaly associated Zika virus (ZIKV) outbreak took place NE during same years. In this work, we tested hypothesis that saxitoxin (STX), a neurotoxin produced South America by freshwater cyanobacteria...
Neurological and other systemic complications occur in adults with severe COVID-19. Here we describe SARS-CoV-2 infection complicated by neuroinvasion the post-mortem tissues of a child.We performed complete autopsy 14-month-old child who died COVID-19 pneumonitis. Histological sections multiple organs were stained haematoxylin eosin. Luxol fast blue staining for myelin immunohistochemistry selected areas brain. The presence was investigated immunostaining anti-spike protein antibody...
Coronavirus disease 2019 (COVID-19) was initially described as a viral infection of the respiratory tract. It is now known, however, that several other organs are affected, including brain. Neurological manifestations such stroke, encephalitis, and psychiatric conditions have been reported in COVID-19 patients, but neurotropic potential virus still debated. Herein, we sought to investigate SARS-CoV-2 human neural cells. We demonstrated tissue non-permissive, it can elicit inflammatory...
<i>Dyrk1a</i> triplication in Down9s syndrome and its overexpression Alzheimer9s disease suggest a role for increased DYRK1A activity the abnormal metabolism of APP. Transport defects are early phenotypes progression disease, which lead to APP processing impairments. However, whether regulates intracellular transport delivery human neurons remains unknown. From proteomic dataset cerebral organoids treated with harmine, inhibitor, we found expression changes protein clusters associated...
Abstract Here a novel technique for engineering neural tissue consisting of motor neurons by combining human‐induced pluripotent stem cells (hiPSCs) with small molecules releasing microspheres is demonstrated. First, the molecule purmorphamine (puro) successfully encapsulated into poly ε‐caprolactone (PCL) using single emulsion oil‐in‐water (o/w) method first time an efficiency (84% ± 2.12%). These release 91% 1.7% puro in controlled fashion over 46 days. Puro microspheres, along previously...
Coronavirus disease 2019 (COVID-19) was initially characterized as a respiratory illness. Neurological manifestations were reported mostly in severely affected patients. Routes for brain infection and the presence of virus particles situ have not been well described, raising controversy about how causes neurological symptoms. Here, we report autopsy findings 1-year old infant with COVID-19. In addition to pneumonitis, meningitis multiple organ damage related thrombosis, previous...
Abstract The northeast (NE) region of Brazil commonly goes through drought periods, which favor cyanobacterial blooms, capable producing neurotoxins with implications for human and animal health. most severe dry spell in the history occurred between 2012 2016. Coincidently, highest incidence microcephaly associated Zika virus (ZIKV) outbreak was described NE during same years. In this work, we tested hypothesis that saxitoxin (STX), a neurotoxin produced South America by freshwater...
Age increases the risk for cognitive impairment and is single major factor Alzheimer's disease (AD), most prevalent form of dementia in elderly. The pathophysiological processes triggered by aging that render brain vulnerable to involve, at least part, changes inflammatory mediators. Here we show lipoxin A4 (LXA4), a lipid mediator inflammation resolution known stimulate endocannabinoid signaling brain, reduced central nervous system. We demonstrate genetic suppression 5-lipoxygenase...
Neural development represents a dynamic process where mitochondrial integrity is decisive for neuronal activity. Structural changes in these organelles may be related to neurological disorders. Valproic acid (VPA) an anticonvulsive drug commonly used epilepsy treatment and its use associated increased risk of neuropsychiatric Recently we showed shape membrane potential mitochondria from human neural progenitor cells (NPCs) exposed VPA (da Costa et al. 2015). Here, applied transmission...
Abstract Psychedelic agonists of serotonin receptors induce neural plasticity and synaptogenesis, but their potential to enhance learning remains uncharted. Here we show that a single dose d-LSD, potent serotonergic agonist, increased novel object preference in young adult rats several days after treatment. d-LSD alone did not increase old animals, could rescue it levels when followed by 6-day exposure enriched environment (EE). Mass spectrometry-based proteomics human brain organoids...
Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which can infect several organs, especially impacting capacity. Among the extrapulmonary manifestations of COVID-19 myocardial injury, associated with a high risk mortality. Myocardial directly or indirectly SARS-CoV-2 infection, be triggered inflammatory processes that lead to damage heart tissue. Since one hallmarks “cytokine storm”, strategies control inflammation infection have...
SARS-CoV-2 infects cardiac cells and causes heart dysfunction. Conditions such as myocarditis arrhythmia have been reported in COVID-19 patients. The Sigma-1 receptor (S1R) is a ubiquitously expressed chaperone that plays central role cardiomyocyte function. S1R has proposed therapeutic target because it may affect replication; however, the impact of inhibition human cardiomyocytes remains to be described. In this study, we investigated consequences iPSC-derived (hiPSC-CM). infection...
Abstract Coronavirus disease 2019 (COVID-19) was initially described as a viral infection of the respiratory tract. It is now known, however, that several other organs are affected, including brain. Neurological manifestations such stroke, encephalitis, and psychiatric conditions have been reported in COVID-19 patients, but neurotropic potential virus still debated. Herein, we sought to investigate SARS-CoV-2 human neural cells. We demonstrated tissue non-permissive, it can elicit...
ABSTRACT Heart dysfunction, represented by conditions such as myocarditis and arrhythmia, has been reported in COVID-19 patients. Therapeutic strategies focused on the cardiovascular system, however, remain scarce. The Sigma-1 receptor (S1R) recently proposed a therapeutic target because its inhibition reduces SARS-CoV-2 replication. To investigate role of S1R infection heart, we used human cardiomyocytes derived from induced pluripotent stem cells (hiPSC-CM) an experimental model. Here show...
Abstract Mitochondrial dysfunction is a central component in the pathophysiology of multiple neuropsychiatric and degenerative disorders. Evaluating mitochondrial function human‐derived neural cells can help characterize dysregulation oxidative metabolism associated with onset brain disorders, may also define targeted therapies. Astrocytes play number different key roles brain, being implicated neurogenesis, synaptogenesis, blood‐brain‐barrier permeability, homeostasis, and, consequently,...
ABSTRACT Proteomic analysis of human cerebral organoids may reveal how psychedelics regulate biological processes, shedding light on drug-induced changes in the brain. This study elucidates proteomic alterations induced by lysergic acid diethylamide (LSD) organoids. By employing high-resolution mass spectrometry-based proteomics, we quantitatively analyzed differential abundance proteins exposed to LSD. Our findings indicate proteostasis, energy metabolism, and neuroplasticity-related...
Proteomic analysis of human cerebral organoids may reveal how psychedelics regulate biological processes, shedding light on drug-induced changes in the brain. This study elucidates proteomic alterations induced by lysergic acid diethylamide (LSD) organoids. By employing high-resolution mass spectrometry-based proteomics, we quantitatively analyzed differential abundance proteins exposed to LSD. Our findings indicate proteostasis, energy metabolism, and neuroplasticity-related pathways....
Age increases the risk for cognitive impairment and is single major factor Alzheimer's disease (AD), most prevalent form of dementia in elderly. The pathophysiological processes triggered by aging that render brain vulnerable to involve, at least part, changes inflammatory mediators. Here we show lipoxin A4 (LXA4), a lipid mediator inflammation resolution known stimulate endocannabinoid signaling brain, reduced central nervous system. We demonstrate genetic suppression 5-lipoxygenase...
Abstract Harmine is a β-carboline found in Banisteriopsis caapi , constituent of ayahuasca brew. Ayahuasca consumed as beverage native Americans’ sacred rituals and religious ceremonies Brazil. Throughout the years, beneficial effects to improve mental health life quality have been reported, which propelled investigation its therapeutic potential target neurological disorders such depression anxiety. Indeed, antidepressant described, raising question cellular mechanisms might underlie those...
Abstract Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which can infect several organs, especially impacting capacity. Among the extrapulmonary manifestations of COVID-19 myocardial injury, associated with a high risk mortality. Myocardial directly or indirectly SARS-CoV-2 infection, be triggered inflammatory processes that cause damage to heart tissue. Since one hallmarks “cytokine storm”, strategies control inflammation...