A5028413988- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Genetics, Aging, and Longevity in Model Organisms
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Yersinia bacterium, plague, ectoparasites research
- Bacillus and Francisella bacterial research
- DNA Repair Mechanisms
- Advanced biosensing and bioanalysis techniques
- Malaria Research and Control
- Plant Disease Resistance and Genetics
- Poxvirus research and outbreaks
- Fungal and yeast genetics research
- RNA modifications and cancer
- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Vector-borne infectious diseases
- Advanced Proteomics Techniques and Applications
- RNA Interference and Gene Delivery
- Insect symbiosis and bacterial influences
- DNA and Nucleic Acid Chemistry
- Virology and Viral Diseases
- Influenza Virus Research Studies
- Herpesvirus Infections and Treatments
Takeda (United States)
2014-2019
Infectious Disease Research Institute
2018
Deerfield (United States)
2015-2016
Takeda (Japan)
2014
Heska (United States)
1996-2007
Collins College
2004
Harvard University
1975-1996
University of Colorado Boulder
1985
Stanford University
1979-1982
Stanford Medicine
1979-1980
A set of vector DNAs (Y vectors) useful for the cloning DNA fragments in Saccharomyces cerevisiae (yeast) and Escherichia coli are characterized. With these vectors, three modes yeast transformation defined. (i) Vectors containing chromosomal sequences (YIp1, YIp5) transform cells at low frequency (1--10 colonies per microgram) integrate into genome by homologous recombination; this recombination is reversible. (ii) Hybrids endogenous plasmid (YEp2, YEp6) much higher (5000--20,000...
A selective scheme is presented for isolating sequences capable of replicating autonomously in the yeast Saccharomyces cerevisiae. YIp5, a vector that contains gene ura3, does not transform ura3 deletion mutant to Ura+. Hybrid YIp5-Escherichia coli DNA molecules also fail produce transformants. However, collections molecular hybrids between YIp5 and from any six eukaryotes tested (S. cerevisiae, Neurospora crassa, Dictyostelium discoideum Ceanhorabditis elegans, Drosophila melanogaster, Zea...
DNA was introduced into the germ line of nematode Caenorhabditis elegans by microinjection. Approximately 10% injected worms gave rise to transformed progeny. Upon injection, supercoiled molecules formed a high-molecular-weight array predominantly composed tandem repeats sequence. Injected linear both and inverted as if they had ligated each other. No worm sequences were required in plasmid for formation these arrays. Surprisingly, arrays extrachromosomal heritable. On average 50% progeny...
DNA was introduced into the germ line of nematode Caenorhabditis elegans by microinjection. Approximately 10% injected worms gave rise to transformed progeny. Upon injection, supercoiled molecules formed a high-molecular-weight array predominantly composed tandem repeats sequence. Injected linear both and inverted as if they had ligated each other. No worm sequences were required in plasmid for formation these arrays. Surprisingly, arrays extrachromosomal heritable. On average 50% progeny...
The par genes participate in the process of establishing cellular asymmetries during first cell cycle Caenorhabditis elegans development. par-2 gene is required for unequal cleavage and length spindle orientation two resulting daughter cells. We have found that PAR-2 protein present adult gonads early embryos. In gonads, uniformly distributed at cortex, this subcellular localization depends on microfilaments. one-cell embryo, localized to posterior cortex partitioned into daughter, P1,...
Tyl is a repeated, transposable DNA element in the genome of yeast Saccharomyces cerevisiae (Cameron et al. 1979). has structure (shown Fig. 1) where δs are direct terminal repeats 338 bp (Gafner and Philippsen 1980). The entire repeated about 30 times haploid genome. In this paper RNA homologous to characterized. There two, large, abundant RNAs DNA, amount larger varies cells different mating type.
Chikungunya virus (CHIKV) is a reemerging mosquito-borne pathogen that has recently caused devastating urban epidemics of severe and sometimes chronic arthralgia. As with most other viral diseases, control relies on reducing mosquito populations their contact people, which been ineffective in locations. Therefore, vaccines remain the best strategy to prevent vector-borne diseases. Ideally, for diseases resource-limited countries should combine low cost single dose efficacy, yet induce rapid...
Since the first demonstration of in vivo gene expression from an injected RNA molecule almost two decades ago,1Wolff J.A. Malone R.W. Williams P. Chong W. Acsadi G. Jani A. Felgner P.L. Direct transfer into mouse muscle vivo.Science. 1990; 247: 1465-1468Crossref PubMed Scopus (3242) Google Scholar field RNA-based therapeutics is now taking significant strides, with many cancer and infectious disease targets entering clinical trials.2Kaczmarek J.C. Kowalski P.S. Anderson D.G. Advances...
Three tetravalent formulations of chimeric dengue (DENVax) viruses containing the pre-membrane and envelope genes serotypes 1–4 expressed by attenuated DENV-2 PDK-53 genome were tested for safety, immunogenicity, efficacy in cynomolgus macaques ( Macaca fascicularis ). Subcutaneous injection DENVax was well-tolerated. Low levels viremia only one four vaccine detected yet virus neutralizing antibody titers induced against all after or two administrations vaccine. All animals immunized with...
Antibody directed against rho factor from vegetative Bacillus subtilis was prepared by immunizing a rabbit with denaturated polypeptide isolated electrophoresis of partially purified DNA-dependent RNA polymerase on sodium dodecyl sulfate-polyacrylamide slab gel. Antiserum to reacted specifically native but not core as judged complement fixation and an immunodiffusion assay. Anti-rho antibody also inhibited the ability stimulate transcription phage phie DNA failed inhibit poly(dA-dT) enzyme....
Background. A safe, effective tetravalent dengue vaccine is a global health priority. The safety and immunogenicity of live attenuated, recombinant candidate (TDV) were evaluated in healthy volunteers from dengue-endemic countries.
Dengue viruses (DENVs) infect >300 million people annually, causing 96 cases of dengue disease and 22 000 deaths [1]. A safe vaccine that protects against DENV is a global health priority [2].