A5107341610- Protein purification and stability
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Drug Solubulity and Delivery Systems
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Inhalation and Respiratory Drug Delivery
- Cell Image Analysis Techniques
- SARS-CoV-2 and COVID-19 Research
- Tuberculosis Research and Epidemiology
- Topological and Geometric Data Analysis
- Advanced biosensing and bioanalysis techniques
- Microencapsulation and Drying Processes
- Bacterial Genetics and Biotechnology
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Crystallization and Solubility Studies
- Enzyme Structure and Function
- Metabolomics and Mass Spectrometry Studies
- bioluminescence and chemiluminescence research
- Cephalopods and Marine Biology
- Spectroscopy Techniques in Biomedical and Chemical Research
- Escherichia coli research studies
- Carbon Nanotubes in Composites
- Molecular Biology Techniques and Applications
Infectious Disease Research Institute
2013-2023
Access to Wholistic and Productive Living Institute
2022
Access to Advanced Health Institute
2022
Wright-Patterson Air Force Base
2004-2020
United States Air Force
2019
Pennsylvania State University
2018
University of Cincinnati
2011-2014
University of Kansas
2013-2014
United States Air Force Research Laboratory
2003-2013
Texas A&M University
2012
On treatment with copper(I) ions, mixtures of the [oligo(2,2')-bipyridine] strands 2-5 yield spontaneously double helicates 2H(2)-2H(5) without significant crossover. Similarly, when a mixture two tris-bypyridine ligands 1 and 3a (containing different substitutions) is allowed to react nickel(II) only helicate 2H(3a) triple 3H(1) are formed. Thus, these systems undergo self-assembly self-recognition. The process represents self/nonself-discrimination involving preferential binding like metal...
Self-assembled particles of genetically engineered human L subunit ferritin expressing a silver-binding peptide were used as nanocontainers for the synthesis silver nanoparticles. The inner cavity self-assembled protein cage displays dodecapeptide that is capable reducing ions to metallic silver. This chimeric when incubated in presence nitrate exhibits growth nanocrystal within its cavity. Our studies indicate it possible design cages, using specific templates, other inorganic
Since the first demonstration of in vivo gene expression from an injected RNA molecule almost two decades ago,1Wolff J.A. Malone R.W. Williams P. Chong W. Acsadi G. Jani A. Felgner P.L. Direct transfer into mouse muscle vivo.Science. 1990; 247: 1465-1468Crossref PubMed Scopus (3242) Google Scholar field RNA-based therapeutics is now taking significant strides, with many cancer and infectious disease targets entering clinical trials.2Kaczmarek J.C. Kowalski P.S. Anderson D.G. Advances...
The development of vaccines containing adjuvants has the potential to enhance antibody and cellular immune responses, broaden protective immunity against heterogeneous pathogen strains, enable antigen dose sparing, facilitate efficacy in immunocompromised populations. Nevertheless, structural interplay between adjuvant components is often not taken into account published literature. Interactions formulations should be well characterized optimum vaccine stability efficacy. This review focuses...
Current RNA vaccines against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) are limited by instability of both the and lipid nanoparticle delivery system, requiring storage at -20°C or -70°C compromising universally accessible vaccine distribution. This study demonstrates thermostability adaptability a nanostructured carrier (NLC) system for that has potential to address these concerns. Liquid NLC alone is stable refrigerated temperatures ≥1 year, enabling stockpiling rapid...
Many cephalopods exhibit remarkable dermal iridescence, a component of their complex, dynamic camouflage and communication. In the species Euprymna scolopes , light-organ iridescence is static due to reflectin protein-based platelets assembled into lamellar thin-film reflectors called iridosomes, contained within iridescent cells iridocytes. Squid in family Loliginidae appear be unique which dermis possesses with reflective, coloured structures that are disassembled under control muscarinic...
The Gcn5 histone acetyltransferase (HAT) subunit of the SAGA transcriptional coactivator complex catalyzes acetylation H3 and H2B N-terminal tails, posttranslational modifications associated with gene activation. Binding partner Ada2 to activates Gcn5’s intrinsically weak HAT activity on proteins, but mechanism for this activation by SANT domain has remained elusive. We have employed Fab antibody fragments as crystallization chaperones determine crystal structures a yeast Ada2/Gcn5 complex....
Abstract The Shigella flexneri Type III secretion system (T3SS) senses contact with human intestinal cells and injects effector proteins that promote pathogen entry as the first step in causing life threatening bacillary dysentery (shigellosis). apparatus (T3SA) consists of an anchoring basal body, exposed needle, a temporally assembled tip complex. Exposure to environmental small molecules recruits IpaB, hydrophobic translocator protein, maturing IpaB then host cell membrane, forming...
Abstract The involvement of innate receptors that recognize pathogen- and danger-associated molecular patterns is critical to programming an effective adaptive immune response vaccination. synthetic TLR4 agonist glucopyranosyl lipid adjuvant (GLA) synergizes with the squalene oil-in-water emulsion (SE) formulation induce strong responses. Although signaling through MyD88 TIR domain–containing adapter inducing IFN-β are essential for GLA-SE activity, mechanisms underlying synergistic activity...
Adjuvants have the potential to increase efficacy of protein-based vaccines but need be maintained within specific temperature and storage conditions. Lyophilization can used thermostability protein pharmaceuticals; however, no marketed vaccine that contains an adjuvant is currently lyophilized, lyophilization oil-in-water nanoemulsion adjuvants presents a challenge. We previously demonstrated feasibility lyophilizing candidate adjuvanted against Mycobacterium tuberculosis (Mtb), ID93 +...
The diameter of single walled carbon nanotubes (SWNTs) determines the electronic properties nanotube. is dictated by catalyst particle. Here we describe use iron nanoparticles synthesized within Dps protein cage as catalysts for growth single-walled nanotubes. discrete particles cages when used gives rise to with a limited distribution.
Converting a vaccine into thermostable dry powder is advantageous as it reduces the resource burden linked with cold chain and provides flexibility in dosage administration through different routes. Such presentation may be especially useful development of towards respiratory infectious disease tuberculosis (TB). This study assesses immunogenicity protective efficacy spray-dried ID93+GLA-SE, promising TB candidate, against Mycobacterium (Mtb) murine model when administered via Four routes...
High-throughput screening (HTS) of 42 865 compounds was performed to identify that inhibit formation or kill Staphylococcus epidermidis RP62a biofilms. Three biological processes were assayed, including (1) growth planktonic/biofilm bacteria, (2) assessment metabolically active biofilm bacteria using a resazurin assay, and (3) biomass by crystal violet staining. After completing the three tiers (primary screening, hit confirmation, dose-response curves), 352 (representing ~0.8%) selected as...
Abstract Current RNA vaccines against SARS-CoV-2 are limited by instability of both the and lipid nanoparticle delivery system, requiring storage at −20°C or −70°C compromising universally accessible vaccine distribution. This study demonstrates thermostability adaptability a nanostructured carrier (NLC) system for use in pandemic preparedness response. Liquid NLC is stable refrigerated temperatures ≥ 1 year, enabling stockpiling rapid deployment point-of-care mixing with any RNA....
Shigella spp. are the causative agent of shigellosis, second leading cause diarrhea in children ages 2-5. Despite many years research, a protective vaccine has been elusive. We recently demonstrated that invasion plasmid antigens B and D (IpaB IpaD) provide protection against S. flexneri sonnei. These proteins, however, have very different properties which must be recognized then managed during formulation. Herein, we employ spectroscopy to assess stability IpaB as well IpgC (invasion...