A5046836603- Advanced Proteomics Techniques and Applications
- Malaria Research and Control
- Mass Spectrometry Techniques and Applications
- Viral Infections and Vectors
- Metabolomics and Mass Spectrometry Studies
- Mosquito-borne diseases and control
- Helminth infection and control
- Trypanosoma species research and implications
- Vector-borne infectious diseases
- Bird parasitology and diseases
- SARS-CoV-2 detection and testing
- Syphilis Diagnosis and Treatment
- Analytical chemistry methods development
- Reproductive tract infections research
- Genital Health and Disease
- Clinical Laboratory Practices and Quality Control
- Protein purification and stability
- Pharmacological Effects and Assays
University of North Carolina at Chapel Hill
2022-2024
Digital Proteomics (United States)
2024
Université de Montréal
2024
Office of Infectious Diseases
2022
The recent surge of coronavirus disease 2019 (COVID-19) hospitalizations severely challenges healthcare systems around the globe and has increased demand for reliable tests predictive severity mortality. Using multiplexed targeted mass spectrometry assays on a robust triple quadrupole MS setup which is available in many clinical laboratories, we determined precise concentrations hundreds proteins metabolites plasma from hospitalized COVID-19 patients. We observed clear distinction between...
Abstract Background Though Plasmodium vivax is the second most common malaria species to infect humans, it has not traditionally been considered a major human health concern in central Africa given high prevalence of Duffy-negative phenotype that believed prevent infection. Increasing reports asymptomatic and symptomatic infections individuals throughout raise possibility P. evolving evade host resistance, but there are few parasite samples with genomic data available from this part world....
Multiple reaction monitoring (MRM) is a key tool for biomarker validation and the translation of potential biomarkers into clinic.
Plasma is an important biofluid for clinical research and diagnostics. In the clinic, unpredictable delays—from minutes to hours—between blood collection plasma generation are often unavoidable. These delays can potentially lead protein degradation modification might considerably affect intact measurement methods such as sandwich enzyme-linked immunosorbent assays that bind proteins on two epitopes increase specificity, thus requiring largely structures. Here, we investigated, using multiple...
Acute febrile illness is a common problem managed by clinicians and health systems globally, particularly in the Tropics. In many regions, malaria leading potentially deadly cause of fever; however, myriad alternative etiologies exist. Identifying fever allows optimal management, but this depends on factors including thorough knowledge circulating infections. Arboviruses such as dengue (DENV) may be underdiagnosed sub-Saharan Africa where major focus. We examined cases western Cameroon that...
Abstract Plasmodium ovale curtisi ( Poc) and wallikeri Pow ) are relapsing malaria parasites endemic to Africa Asia that were previously thought represent a single species. Amid increasing detection of in sub-Saharan Africa, we performed population genomic study both species across the continent. We conducted whole-genome sequencing 25 isolates from Central East analyzed them alongside 20 published African genomes. Isolates predominantly monoclonal (43/45), with their genetic similarity...
Abstract Plasmodium ovale curtisi ( Poc) and wallikeri Pow ) are relapsing malaria parasites endemic to Africa Asia that were previously thought represent a single species. Amid increasing detection of in sub-Saharan Africa, we present population genomic study both species across the continent. We conducted whole-genome sequencing 25 isolates from Central East analyzed them alongside 20 published African genomes. Isolates predominantly monoclonal (43/45), with their genetic similarity...
Genital ulcer diseases (GUDs) are a common syndrome associated with sexually transmitted infections. increase the risk of HIV transmission, necessitating appropriate diagnosis and treatment. We provide an updated GUD etiology assessment in Malawi to guide diagnostic development treatment algorithms.
Synthetic peptides are a critical requirement for the development and application of targeted mass spectrometry (MS)-based assays quantitation proteins from biological matrices. Transporting synthetic on dry ice one laboratory to another is costly often difficult because country-specific import export regulations. Therefore, in this study, we assessed impact leaving lyophilized mixture consisting 125 at room temperature up 20 days, effect quantitative performance multiple reaction...
Background: Malaria remains a major cause of morbidity in sub-Saharan Africa. Undetected asymptomatic falciparum malaria results large transmission reservoir and there is evidence increasing non-falciparum as controlled Africa, both resulting challenges for control programs. Methods: We performed quantitative real time PCR 4 species 4,596 individuals from the 2014-2015 Rwanda Demographic Health Survey. Bivariate models were used to determine species-specific associations with risk factors....
Abstract Relative quantitation, used by most MS-based proteomics laboratories to determine protein fold-changes , requires samples being processed and analyzed together for best comparability through minimizing batch differences. This limits the adoption of in population-wide studies, detection subtle but relevant changes heterogeneous samples. Absolute quantitation circumvents these limitations enables comparison results across laboratories, longitudinally. However, high costs essential...
Absolute LC/MRM-MS based quantitation targeting 270 proteins yielded 44% more quantifiable when combined with automated nanoparticle (NP)-based fractionation and digestion approach as compared to neat trypsin digestion.
Abstract The recent surge of COVID-19 hospitalizations severely challenges healthcare systems around the globe and demands for reliable tests predictive disease severity mortality. Using multiplexed targeted mass spectrometry assays on a robust triple quadrupole MS setup which is available in many clinical laboratories, we determined precise concentrations 100s proteins metabolites plasma from hospitalized patients. We observed clear distinction between patients controls and, strikingly,...
Abstract Plasmodium vivax malaria has not traditionally been a major concern in central Africa given the high prevalence of human Duffy-negative phenotype that is believed to prevent infection. Increasing reports asymptomatic and symptomatic infections individuals throughout raise possibility P. evolving evade host resistance, but there are few parasite samples with genomic data available from this part world. In study, we perform whole genome sequencing new isolate Democratic Republic Congo...